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Double-amplification electrochemiluminescence sensor and method for determining chlorpyrifos

A chlorpyrifos and sensor technology, applied in the field of pesticide residue determination, can solve problems such as a single signal amplification method, and achieve the effect of improving detection selectivity, low density, and high sensitivity detection

Pending Publication Date: 2022-01-25
UNIV OF JINAN
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Problems solved by technology

In the process of construction and detection of electrochemical sensors, researchers designed a variety of analysis strategies for the sensitivity of the sensor, such as steric hindrance, energy transfer, enzyme inhibition reaction and various signal amplification strategies, which can amplify the ECL signal through various channels. , but most of these signal amplification strategies use a single signal amplification method

Method used

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  • Double-amplification electrochemiluminescence sensor and method for determining chlorpyrifos

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Effect test

Embodiment 1

[0027] The preparation of embodiment 1 hollow Fe / Co-MOF:

[0028] (1) Synthesis of PS spheres: 30 mL ultrapure water was placed in a three-necked flask, N 2 After deoxygenation, 1.5 g of polyvinylpyrrolidone (PVP) and 0.015 g of potassium persulfate were added in sequence, and after complete dissolution, 3 mL of styrene was added and heated at 70 °C for 24 h. Wash with water and ethanol and separate the product, disperse the product in 5 mL of ethanol to obtain a PS sphere dispersion, add 0.5 g of PVP to the dispersion and stir for 12 h, after the reaction is complete, the product is fully washed with ethanol and methanol, and then dispersed in 10 mL of methanol solution to obtain methanol solution of PVP-modified PS spheres;

[0029] (2) Synthesis of FeOOH nanorods: 0.2 mL polyethyleneimine was added to 100 mL 20 mM FeCl 3 •6H 2 O solution, heated and stirred at 80 °C for 2 h, and centrifuged and washed three times with ultrapure water to obtain FeOOH nanorods. Disperse F...

Embodiment 2

[0031] Example 2 Preparation of double-amplified aptasensors:

[0032] (1) Take 3 mL of 5 mM AgNO 3 and 0.5 mL of 0.01 M luminol solution were added to a flask filled with 9.0 mL of absolute ethanol and 5.0 mL of ultrapure water, and then 0.1 mL of 0.1 M NaOH was quickly added and stirred for 4 h. When the solution color turned dark yellow, the mixture was fully washed with ethanol, and the product was dispersed in 5 mL of ethanol to obtain a Lu-Ag ethanol solution;

[0033] (2) Add 1 mL of 0.125 g / L Fe / Co-MOF ethanol solution and 500 μL Lu-Ag ethanol solution into the flask. Shake on a shaker for 30 min, then incubate at 4 °C for 12 h. A hollow Fe / Co-MOF-Lu-Ag composite was obtained. Take 80 μL of 1 μM chlorpyrifos aptamer and add to 800 μL of 0.125 g / L Fe / Co-MOF-Lu-Ag ethanol solution. Shake on a shaker for 30 min, then incubate at 4 °C for 12 h to prepare Apt-Ag-Lu-Fe / Co-MOF;

[0034] (3) The treated glassy carbon electrode GCE was plated with gold at a constant potent...

Embodiment 3

[0037] Example 3 Method for detecting chlorpyrifos with double-amplified aptasensors:

[0038] (1) Add different concentrations of chlorpyrifos pesticide dropwise on the surface of the constructed sensor, incubate at 37 °C for 1 h, and wash with PBS buffer at pH 7.8;

[0039] (2) The aptamer sensor was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum electrode was used as the auxiliary electrode; 2 o 2 In the buffer solution of 0.1 M pH 7.8 PBS, cycle in the potential range of 0.0-0.7 V, scan the photomultiplier tube at 600 V, and record the luminous intensity;

[0040] (3) Detect the light intensity of a series of standard solutions of chlorpyrifos with different concentrations, and draw a working curve; at the same time, measure the linear range and detection limit of the sensor. The intensity of electrochemiluminescence decreases with the increase of chlorpyrifos concentration, the linear equation is DI=-359.57 lg...

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Abstract

The invention relates to the technical field of pesticide residue analysis and determination, in particular to a double-amplification aptamer sensor based on hollow Fe / Co-MOF and Ag NPs and a method for determining chlorpyrifos by using the sensor, and the method comprises a double-amplification sensor preparation step, an operation method for determining chlorpyrifos by using the sensor and the like. According to the sensor, the catalytic action of the hollow Fe / Co-MOF on a luminol-H2O2 system and the enhanced luminescence effect of the nano-silver on luminol-H2O2 are utilized, ECL luminescence is jointly enhanced, and the sensor is used for detecting chlorpyrifos and is high in sensitivity and good in specificity.

Description

technical field [0001] The present invention relates to the technical field of pesticide residue determination, in particular to a double signal amplification electrochemiluminescence sensor and a method for chlorpyrifos, including the preparation steps of the double signal amplification sensor and the operation method of using the sensor to measure chlorpyrifos, etc.; Dual signal amplification sensor utilizing hollow Fe / Co-MOF for luminol-H 2 o 2 The catalytic effect of the system, and the effect of nano-silver on luminol-H 2 o 2 Enhanced luminescence, to achieve combined enhanced ECL luminescence, used for the detection of chlorpyrifos, with high sensitivity and good specificity. Background technique [0002] With the needs of social production and health, the detection of pesticide residues has attracted widespread attention. Traditional pesticide residue detection technologies are costly and time-consuming, so it is very important to develop a rapid detection technolo...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 马东晓颜梅刘汉彪衣姜乐夏方诠田栋
Owner UNIV OF JINAN
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