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Bone repair scaffold material capable of mediating nervalization and preparation method and application thereof

A scaffold material and bone repair technology, applied in the field of biomedical composite materials, can solve problems such as affecting the quality of life of patients, no feeling at the repair site, etc., and achieve the effect of promoting the process of bone repair, promoting the survival process, and speeding up the rhythm.

Active Publication Date: 2022-01-28
NAT UNIV OF DEFENSE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that the bone repair materials in the prior art only repair the bone defect without repairing the nerve together, resulting in no sensation at the repaired part after repair, which ultimately affects the quality of life of the patient, the present invention provides a bone repair material that can mediate nerve Repairing scaffold material and its preparation method and application, a bone repairing scaffold material capable of mediating neuralization according to the present invention can realize the simulation effect on the bone matrix scaffolding structure, thereby providing a basis for ossification and neuralization in bone defect repair. Provide a suitable microenvironment to promote rapid repair of bone defects and recovery of sensation

Method used

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  • Bone repair scaffold material capable of mediating nervalization and preparation method and application thereof
  • Bone repair scaffold material capable of mediating nervalization and preparation method and application thereof
  • Bone repair scaffold material capable of mediating nervalization and preparation method and application thereof

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Embodiment 1

[0036] A preparation method of a bone repair scaffold material capable of mediating neuralization, comprising the following steps:

[0037] 1) The heterologously expressed OCN-OPN-BGN fusion protein (OOB fusion protein) in vitro and magnesium-doped mesoporous bioactive glass (Mg-MBG) were mixed and stirred for 1 hour according to the ratio of 200g:20mg, and placed in Stand in the refrigerator at 4°C for 24 hours, centrifuge at 12,000rpm for 5 minutes, and take the precipitate to obtain the Mg-MBG material loaded with OOB fusion protein (OOB@Mg-MBG);

[0038] 2) According to the ratio of 6g:2.2g / L, pass the silkworm cocoon through Na 2 CO 3 After the aqueous solution is boiled for degumming, place it in an oven at 50°C and dry it, and pass through an 8M lithium bromide solution in an oven at 50°C to completely dissolve it to obtain dissolved silk fibroin;

[0039] After the dissolved silk fibroin is dialyzed with deionized water, it is concentrated for 60 hours through a 10% ...

Embodiment 2

[0045] A preparation method of a bone repair scaffold material capable of mediating neuralization, comprising the following steps:

[0046] 1) The heterologously expressed OCN-OPN-BGN fusion protein (OOB fusion protein) in vitro and magnesium-doped mesoporous bioactive glass (Mg-MBG) were mixed and stirred for 2 hours according to the ratio of 100g:20mg, and placed in Put it in a refrigerator at 4°C for 48 hours, centrifuge at 12,000rpm for 5 minutes, and take the precipitate to obtain the Mg-MBG material loaded with OOB fusion protein (OOB@Mg-MBG);

[0047] 2) According to the ratio of 3g:4.4g / L, pass the silkworm cocoon through Na 2 CO 3 After the aqueous solution is boiled for degumming, place it in an oven at 40°C and dry it, and pass through a 10M lithium bromide solution in an oven at 40°C to completely dissolve it to obtain dissolved silk fibroin;

[0048] After the dissolved silk fibroin is dialyzed with deionized water, it is concentrated for 48 hours through a 5% P...

Embodiment 3

[0054] A preparation method of a bone repair scaffold material capable of mediating neuralization, comprising the following steps:

[0055] 1) The heterologously expressed OCN-OPN-BGN fusion protein (OOB fusion protein) and magnesium-doped mesoporous bioactive glass (Mg-MBG) were mixed and stirred for 1.5 hours according to the ratio of 300g:20mg, Put it in a refrigerator at 4°C for 32 hours, centrifuge at 12,000 rpm for 5 minutes, and take the precipitate to obtain the Mg-MBG material loaded with OOB fusion protein (OOB@Mg-MBG);

[0056] 2) According to the ratio of 8g:3.3g / L, pass the silkworm cocoon through Na 2 CO 3 After the aqueous solution is boiled for degumming, place it in an oven at 80°C and dry it, and pass through a 7M lithium bromide solution in an oven at 80°C to completely dissolve it to obtain dissolved silk fibroin;

[0057] After the dissolved silk fibroin is dialyzed by deionized water, it is concentrated by 20% PEG solution with a molecular weight of 100...

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Abstract

The invention discloses a bone repair scaffold material capable of mediating nervalization and a preparation method and application thereof. The preparation method comprises the steps that heterologous expression bone matrix fusion protein is adsorbed into calcium and magnesium doped mesoporous bioactive glass prepared through a sol-gel and template self-assembly method to serve as an inner core; nerve growth factors capable of promoting nerve growth are loaded into silk protein to serve as a shell, and then the bone repair scaffold material which is of a core-shell structure and can be used for mediating nervalization is prepared through a coaxial electrostatic spinning technology. The bone repair scaffold material capable of mediating the nervalization has good cell compatibility, osteogenic activity, neurogenic activity and calcium deposition promotion effects, can promote proliferation, differentiation and mineralization processes of bone marrow mesenchymal stem cells in the osteogenic direction, can also promote the survival process of neurons, as a bone repair material, the material has a wide prospect in clinical application of repairing bone defects and restoring consciousness of injured parts.

Description

technical field [0001] The invention belongs to the technical field of biomedical composite materials, and in particular relates to a bone repair scaffold material capable of mediating neuralization and its preparation method and application. Background technique [0002] The growth and development stages of bones and nerves are consistent, and nerves extend into bone development in a specific form, forming a "nerve-bone" structure. In mature bone, the bone is tightly bound to the nerves. After bone injury, nerve fibers in bone tissue secrete neuropeptides to promote bone repair. In clinical cases of nonunion and delayed healing of bone defects, it is found that there are almost no nerve fibers in the callus. This shows that the neuralization of the bone defect site can significantly promote the process of bone repair, and the neuralization of the bone defect site directly affects the recovery of the patient's motor ability and quality of life, and is an important reference...

Claims

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Application Information

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IPC IPC(8): A61L27/10A61L27/22A61L27/54A61L27/50D01D5/00
CPCA61L27/10A61L27/227A61L27/54A61L27/50D01D5/003A61L2430/02A61L2430/32A61L2300/414A61L2400/12C08L89/00
Inventor 王晓燕柳珑刘浩明林嘉宇白臻祖黄珊郑惟嘉姜凯
Owner NAT UNIV OF DEFENSE TECH
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