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Application of mulberry extract in preparation of medicine for treating pancreas-related diseases

An extract, pancreas technology, applied in metabolic diseases, drug combinations, endocrine system diseases, etc., to achieve the effect of lowering blood sugar

Pending Publication Date: 2022-02-08
BEIJING WEHAND BIO PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its effect and mechanism on islet β cells have not been reported yet.

Method used

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  • Application of mulberry extract in preparation of medicine for treating pancreas-related diseases
  • Application of mulberry extract in preparation of medicine for treating pancreas-related diseases
  • Application of mulberry extract in preparation of medicine for treating pancreas-related diseases

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0089] Take 1000 kg of fresh mulberry branches (Xiaza Sangyuesang No. 11), crush them, add 4000 L of water, extract by heating and reflux for 2 hours, combine the extracts, filter to remove insoluble matter, and obtain a crude extract. The crude extract was heat-concentrated until the mass percentage of solid matter reached 4%, and kept at 50° C. as the sample solution for the cationic resin column.

[0090] Use 150kg of D113 type macroporous weakly acidic styrene-acrylic cationic resin to pack the column, use 2mol / L hydrochloric acid solution to wash until the pH of the eluate is 4.5; use 1mol / L sodium hydroxide solution to wash until the pH of the eluate is 8.5; 2mol / L hydrochloric acid solution until the pH of the eluate is 4.5; then rinse with 5 times the column volume of deionized water to complete the activation. Load the concentrated extract, then use 1000L 2.5mol / L ammonia water to elute, the elution rate is 6BV / h, and collect the eluate when the pH of the cationic co...

preparation example 2

[0095] Take 10kg of fresh mulberry branches (Sangteyou No. 2), crush them, add 150L of water, add in 2 times, extract by decoction for 3 hours each time, combine the extracts, and filter to remove insoluble matter. The extract was thermally concentrated until the solid content reached 8% by mass, and then transferred to an alcohol precipitation tank, and 2367.9 g of absolute ethanol (3 L) was added at a stirring blade of 300 rpm. After the ethanol was added, the stirring was stopped, and the alcohol was precipitated for 24 hours, and the supernatant was taken as the sample solution for the cationic resin column. Use 002SC type strongly acidic styrenic cationic resin 5kg packing column, according to the method for embodiment 1, cationic resin is activated. Load the extract that has been concentrated and alcohol-precipitated, and then use 100L 5mol / L potassium chloride to elute at a rate of 5BV / h. Use 20% silicotungstic acid to detect the effluent, and start collecting when whit...

preparation example 3

[0102] Take 1000 kg of fresh mulberry branches (Guangdong mulberry), crush them, add 11500 L of water, heat and reflux for extraction for 2 hours, combine the extracts, filter to remove insoluble matter, and obtain a crude extract. The crude extract is first centrifuged to remove impurities, and then concentrated with a counter ion osmosis membrane until the solid content reaches 1% by weight, and is used as the sample solution for the cationic resin column.

[0103] Use 300 kg of D001 type macroporous strongly acidic styrene-based cationic resin to pack into a column, and activate the cationic resin according to the method of Preparation Example 1. Load the concentrated crude extract, use 5000L 0.04mol / L ammonium nitrate to elute, the elution rate is 5BV / h, use 20% silicotungstic acid to detect the effluent, start to collect when white precipitate is formed, when collected When the liquid reaches 1000L, stop collecting.

[0104] Concentrating the collected solution obtained ...

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Abstract

The invention discloses application of a mulberry extract. The application disclosed by the invention is the application of the mulberry extract or main active ingredients thereof in preparation of products for preventing and / or treating pancreas-related diseases. Experiments prove that the mulberry extract has the following effects: promoting insulin secretion of beta cells and primary islet; increasing [Ca < 2 + >] i of MIN6 cells; changing inhibiting effect of glucolipotoxicity on the growth of MIN6 cells; reversing dedifferentiation of beta cells in the pancreas islet; reducing blood glucose of animals with spontaneous type 2 diabetes mellitus, and promoting insulin secretion; improving the pancreas islet structure of a spontaneous type 2 diabetes animal; improving the pancreatic hyperfunction and the pancreatic islet compensatory increase.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of mulberry extracts in the preparation of medicines for treating pancreas-related diseases. Background technique [0002] The pancreas is an important digestive organ and endocrine organ of the human body. It realizes its functions through its two parts, the exocrine gland and the endocrine gland. The exocrine gland is composed of acini and glandular ducts. Pancreatic juice contains sodium bicarbonate, trypsinogen, lipase, amylase, etc. Pancreatic juice is discharged into the duodenum through the pancreatic duct, where it can digest protein, fat and sugar. Endocrine glands are composed of cell clusters of different sizes—islets. The islets are mainly composed of 4 types of cells: A cells, B cells, D cells, and PP cells. Among them, pancreatic β-cells are the only cells in the body that can synthesize and secrete insulin, and play an important ro...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61P1/18A61P3/10A61P5/50
CPCA61K36/605A61P1/18A61P3/10A61P5/50A61K2236/55
Inventor 刘玉玲申竹芳雷蕾刘泉陈艳敏刘圆圆环奕李彩娜刘志华朱向阳王婷婷邹媛媛
Owner BEIJING WEHAND BIO PHARMA CO LTD
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