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Molecularly imprinted ratiometric fluorescent probe based on carbon dots as well as preparation method and application thereof

A ratiometric fluorescent probe and molecular imprinting technology, applied in the field of fluorescence sensing and detection, can solve the problems of cumbersome preparation method, secondary environmental pollution, wide emission spectrum, etc., and achieve the effects of wide source of raw materials, low price and simple synthesis method.

Active Publication Date: 2022-02-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the existing ratiometric fluorescent sensors use organic fluorescent dyes or semiconductor quantum dots containing heavy metal elements as the signal source, among which organic fluorescent molecules are easy to photobleach, and the emission spectrum is wide and easy to overlap. Although semiconductor quantum dots have good photochemical stability, they are not easy to Be photolyzed or bleached, but its preparation method is cumbersome, expensive, and easy to cause secondary pollution to the environment

Method used

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  • Molecularly imprinted ratiometric fluorescent probe based on carbon dots as well as preparation method and application thereof
  • Molecularly imprinted ratiometric fluorescent probe based on carbon dots as well as preparation method and application thereof
  • Molecularly imprinted ratiometric fluorescent probe based on carbon dots as well as preparation method and application thereof

Examples

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preparation example Construction

[0049] The invention provides a method for preparing a molecularly imprinted ratiometric fluorescent probe for detecting ciguatera toxin in coral reef fish. The method includes: coating the red carbon dots with a silicon dioxide layer to obtain RCDs@SiO 2 ; then in RCDs@SiO 2 On the surface, monensin is used as a fragment-virtual template molecule, 3-aminopropyltriethoxysilane is used as a functional monomer, and ethyl orthosilicate is used as a cross-linking agent to polymerize the upper molecular imprinting layer, and the blue carbon dots are embedded in the In the molecularly imprinted layer, a spherical material that forms a core-shell structure.

[0050] The molecularly imprinted ratiometric fluorescent probe MIPs@BCDs / RCDs@SiO 2 The preparation method mainly comprises the following steps:

[0051] Step 1: preparation of blue carbon dots (BCDs);

[0052] Step 2: preparation of red carbon dots (RCDs);

[0053] Step 3: Prepare RCDs@SiO by coating RCDs with a silicon di...

Embodiment 1

[0063] Example 1 Molecular imprinting ratiometric fluorescent probe MIPs@BCDs / RCDs@SiO 2 preparation of

[0064] refer to Figure 1 to Figure 6 , the present invention provides a molecularly imprinted ratio fluorescent probe for detecting ciguatera toxin in coral reef fish, said molecularly imprinted ratio fluorescent probe is composed of blue carbon dots, red carbon dots, monensin, 3-ammonia A core-shell structure polymer obtained by the polymerization of propyltriethoxysilane and tetraethylorthosilicate, and the red carbon dots are coated with a silica layer to obtain RCDs@SiO 2 , the synthetic imprinting layers of monensin, 3-aminopropyltriethoxysilane and tetraethyl orthosilicate were grafted on RCDs@SiO 2 The outer layer, the blue carbon dots are embedded in the imprinted layer, and the fragment imprinted ratio fluorescent probe MIPs@BCDs / RCDs@SiO is obtained after monensin is eluted 2 .

[0065] The red carbon dots are located in the inner core as a fluorescent refer...

Embodiment 2

[0079] Example 2 Molecular Imprinting Ratio Fluorescent Probes MIPs@BCDs / RCDs@SiO 2 selectivity

[0080] In order to study the specific adsorption of the molecularly imprinted ratiometric fluorescent probe prepared in Example 1, the present invention further compared and analyzed MIPs@BCDs / RCDs@SiO 2 and non-imprinted ratiometric fluorescent probes NIPs@BCDs / RCDs@SiO 2 Adsorption properties for 6 structural analogues. The specific method is: take 300μL of 0.1mg / mL MIPs@BCDs / RCDs@SiO 2 and NIPs@BCDs / RCDs@SiO 2 solution, add 100 μL 0.1ng / mL monensin, P-CTX-3C, nigericin, rotenone, ionomycin A and lysosin into the solution respectively, put them in a constant temperature oscillator to fix and oscillate, set The oscillation time of the constant temperature oscillator is 10min, the oscillation speed is 200rpm, and the temperature is 25℃, comparing MIPs@BCDs / RCDs@SiO 2 and NIPs@BCDs / RCDs@SiO 2 Adsorption properties for different molecules. Using the degree of fluorescence que...

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Abstract

The invention provides a molecularly imprinted ratiometric fluorescent probe based on carbon dots as well as a preparation method and application of the molecularly imprinted ratiometric fluorescent probe. The molecularly imprinted ratiometric fluorescent probe is a core-shell structure polymer obtained by polymerizing blue carbon dots, red carbon dots, monensin, 3-aminopropyltriethoxysilane and tetraethoxysilane, the red carbon dots are coated with a silicon dioxide layer to obtain RCDs@SiO2. An imprinting layer is synthesized by using monensin, 3-aminopropyltriethoxysilane, tetraethoxysilane and the like, grafted on the outer layer of the RCDs@SiO2, and blue carbon dots are embedded into the imprinting layer to obtain the molecular imprinting ratio fluorescent probe. According to the present invention, the fragment imprinting technology and the carbon dot-based dual-emission ratio fluorescence technology are combined, and the cheap fragment structure (monensin) is adopted as the virtual template of the target molecule (ciguatoxin), such that the cost is saved, the green environmental protection is provided, the operation is simple and convenient, and the specific adsorption and the high-sensitivity rapid detection of the ciguatoxin can be achieved.

Description

technical field [0001] The invention relates to the technical field of fluorescence sensing and detection, in particular to a carbon dot-based molecularly imprinted ratiometric fluorescent probe and its preparation method and application. Background technique [0002] Ciguatoxin is a type of marine biotoxin produced by toxic microalgae in water bodies. It is very toxic, and the toxicity of Pacific Ciguatoxin-3C (P-CTX-3C) is equivalent to 100 times that of tetrodotoxin. . Ciguatera toxins are easy to accumulate in coral reef fish and pass through the food chain to cause human poisoning and even death. The existing detection methods of ciguatoxin are mainly enzyme-linked immunosorbent assay and LC / MS / MS method. Although ELISA has high selectivity, it is limited by the cross-reactivity of antibodies and the properties of biological materials, and its stability is not high; LC / MS / MS method as a confirmatory method has high sensitivity and accuracy, but requires expensive The...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/02C09K11/65B82Y20/00B82Y30/00B82Y40/00C08G77/26
CPCG01N21/645G01N21/6428C09K11/025C09K11/65B82Y20/00B82Y30/00B82Y40/00C08G77/26
Inventor 徐小艳戚镇科田兴国向诚郑曼妮谢创杰
Owner SOUTH CHINA AGRI UNIV
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