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Application of 2-amino-3-hydroxy-3-methylbutyric acid and/or 2-amino-3-(4-hydroxyphenyl) butyric acid

A technology of methyl butyric acid and hydroxyphenyl, which is applied in the field of agricultural biopesticides to achieve the effect of simple structure and convenient biological extraction method

Active Publication Date: 2022-03-15
NANJING TIANNONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is no report about the compound as a natural product

Method used

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  • Application of 2-amino-3-hydroxy-3-methylbutyric acid and/or 2-amino-3-(4-hydroxyphenyl) butyric acid
  • Application of 2-amino-3-hydroxy-3-methylbutyric acid and/or 2-amino-3-(4-hydroxyphenyl) butyric acid
  • Application of 2-amino-3-hydroxy-3-methylbutyric acid and/or 2-amino-3-(4-hydroxyphenyl) butyric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 (biological synthesis, extraction method and structural identification of the compound of the present invention)

[0047] (1) Cultivation of Alternaria

[0048] Glucose sodium nitrate medium: glucose, 40.0g; NaNO 3 , 1.0g; NH 4 Cl, 0.25g; KH 2 PO 4 , 1.0g; KCl, 0.25g; NaCl, 0.25g; MgSO 4 ·7H 2 O, 0.5g; FeSO 4 ·7H 2 O, 0.01g; ZnSO 4 ·7H 2 O, 0.01g; yeast extract, 1g, add water to 1L, adjust pH to 5.5.

[0049] The Alternaria culture method is as follows: PDA medium activates the preserved strains, and after 7 days, select colonies with consistent growth, take a 5mm diameter bacterial cake, and inoculate it into 500mL medium, and the inoculation amount is one bacterial cake per 100mL. Place the culture medium inoculated with the bacterial blocks into a constant temperature shaker, and the culture conditions are: 140 rpm, 25° C., and culture in the dark for 7 days.

[0050] (2) Extraction of compounds

[0051] Mycelia were isolated from the ferment...

Embodiment 2

[0063] The mass spectrum shows that the molecular ion peak of the compound is: 196.0967[M+H] + , determine its molecular formula as: C 10 h 13 NO 3 . Combined with the results of H NMR and C NMR, it was determined that the compound was 2-amino-3-(4-hydroxyphenyl)butyric acid. Example 2 (2-amino-3-hydroxyl-3-methylbutyric acid and 2-amino-3-(4-hydroxyphenyl) butyric acid induce tobacco resistance to tomato spotted wilt virus infection)

[0064] Tomato spotted wilt virus was obtained from Yunnan Province, China. The initial virus source was stored in a -80°C refrigerator. The virus was inoculated on the leaves of Nicotiana benthamiana by friction inoculation to activate the virus, and the virus plasmid was extracted using Escherichia coli competent cells. Transformation, smeared on resistant plates and cultured, picked a single colony for PCR screening, selected positive colonies for sequencing and subsequent plasmid extraction, added the plasmids with normal sequencing to A...

Embodiment 3

[0082] Example 3 (2-amino-3-hydroxy-3-methylbutyric acid and 2-amino-3-(4-hydroxyphenyl) butyric acid induce Arabidopsis resistance to Pseudomonas syringae infection)

[0083] Dissolve 2-amino-3-hydroxy-3-methylbutyric acid in sterile water, and then use sterile water to gradiently dilute it into 100nM, 1000nM and 10000nM solutions. A blank control is also set up, and 0.02% Tween 20 is added at the same time as Surfactant. Spread Pseudomonas syringae PstDC3000 on an LB plate and culture at 28°C for 48 hours; pick a single colony and inoculate it into a 50mL centrifuge tube containing 2mL of medium, culture it on a shaker at 28°C and 250rpm, monitor every 1-2h Bacteria solution OD 600 value change, at OD 600 Stop culturing bacteria before the value reaches 0.8; transfer 1mL of bacterial liquid to a sterile 1.5mL centrifuge tube, centrifuge at 8000rpm for 2min, and collect the precipitate; remove the supernatant, wash the precipitate with 10mM magnesium chloride for 3 times an...

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Abstract

The invention discloses an application of 2-amino-3-hydroxy-3-methylbutyric acid and / or 2-amino-3-(4-hydroxyphenyl) butyric acid, and a preparation method of the 2-amino-3-hydroxy-3-methylbutyric acid. 2-amino-3-hydroxy-3-methylbutyric acid and / or 2-amino-3-(4-hydroxyphenyl) butyric acid are / is used as natural active substances to be developed into the plant immune resistance inducer which can be used for improving the resistance of plants to biological stress and abiotic stress, and infection of fungi, viruses and bacteria on the plants and reduction of the pathogenic level are effectively prevented; meanwhile, the tolerance of plants to high temperature, low temperature, drought and salt stress can be remarkably improved. The 2-amino-3-hydroxy-3-methylbutyric acid and the 2-amino-3-(4-hydroxyphenyl) butyric acid have the characteristics of safety, environmental protection and high efficiency.

Description

technical field [0001] The invention belongs to the field of agricultural biological pesticides, and relates to the application of 2-amino-3-hydroxy-3-methyl butyric acid and / or 2-amino-3-(4-hydroxyphenyl) butyric acid. Background technique [0002] Plant immune inducers are a new class of pesticides, which can enhance plant disease resistance and stress resistance by activating the plant's immune system and regulating plant metabolism. Plant immune inducers themselves have no insecticidal or fungal activity, and are mainly used to stimulate the plant's own natural immune system to prevent and control pests and diseases. Because it does not rely on exogenous pesticides to directly kill pathogens, it is not easy for pests to develop resistance to them, which is in line with the idea of ​​​​realizing green prevention and control under the condition of effectively protecting agricultural biodiversity. In addition, in nature, the growth of plants is usually not only subject to ...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01P21/00A01P1/00A01P3/00
CPCA01N37/44
Inventor 陈世国王赫王良省房婉萍郭爱平
Owner NANJING TIANNONG BIOTECHNOLOGY CO LTD
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