Cerebroprotein hydrolysate, preparation method thereof and composition containing cerebroprotein hydrolysate

Abstract

The invention relates to the field of food processing, and particularly discloses a cerebroprotein hydrolysate, a preparation method thereof and a composition containing the cerebroprotein hydrolysate. The preparation method of the cerebroprotein hydrolysate comprises the following steps: tissue homogenization, enzyme activity inactivation, enzymolysis, high-temperature sterilization, centrifugal concentration, degreasing and post-treatment. Wherein the homogenate is subjected to high-temperature passivation treatment, so that the enzymolysis efficiency can be improved, after the enzymatic hydrolysate is subjected to centrifugal concentration treatment, the use amount of acetone during degreasing can be reduced, finally, residual acetone can be removed through drying treatment, and the preparation method has the advantages of being simple in process, high in degreasing efficiency and high in hydrolysate extraction efficiency. The cerebroprotein hydrolysate can be directly eaten and can also be eaten in cooperation with other raw materials, and the cerebroprotein hydrolysate prepared through the method is fine and smooth in texture, small in fishy smell, light in color, high in amino acid content, polypeptide content, total ganglioside content and total sialic acid content and low in fat and cholesterol content.

Description

Technical field

[0001] The present application relates to the field of food processing, and more specifically, it relates to cerebral protein hydrolyzatics and preparation methods thereof and compositions comprising brain hydrolyzate. Background technique

[0002] Ethylene protein hydrolyza refers to a mixture of hydrolyzed, protein, amino acid, and the like from animal brain tissue, which can act on human neurotic tissue, can improve nerve cell metabolism, promote protein synthesis of neural cells, and protect nerves. Cells are from ischemia and neurotoxin damage, with the role of improving symptoms such as insomnia, headache, memory loss, dizziness and irritability.

[0003] In the relevant technique, the preparation method of brain hydrolyza usually includes the following steps: animal brain tissue → pre-treatment → homogenate → enzymatic hydrolysis → centrifugal → ultrafiltration → column chromatography → sterilization. Due to high fat and cholesterol in the animal brain (suc...

Examples

preparation example Construction

[0053] The present application provides a method of preparing an brain hydrolyzate, including the following steps:

[0054] S1, tissue homogenization: After cleaning the animal brain, remove the meningum and blood vessels, rinse the surface with deionized water, add 2-3 times of deionized water by weight of the animal brain, and then rubbed twice with colloidal grinding. Extremely spray;

[0055] S2, passivation enzyme activity: After boiling the homogenate, maintain 20-40 min, to passivate the enzyme activity and sterilize the animal brain tissue, and then cool the pretreative homogenate;

[0056] S3, enzymatic hydrolysis: The pretreatment homogenate is added to the protease, the adjustment system pH is 6.5-7.5, and the temperature is 55-60 ° C, and the temperature is stirred under temperature under this condition, hydrolyzing hydrolysis is 6-12 h, resulting in an enzymatic hydrolyzide; wherein the protease is papaya. The weight ratio of protease and trypsin and ponybain and anim...

Embodiment 1

[0067] Example 1: A method of preparing an brain hydrolyzate comprising the steps of:

[0068] S1, tissue homogenate: After cleaning fresh pig brain, remove the meningum and blood vessels, flush the surface with deionized water, add 2 times deionized water in the weight of the pig brain, and then rub it twice with colloidal grinding. Homogenate;

[0069] S2, passivation enzyme activity: After boiling the homogenate, maintained for 30 min to passivate the enzyme activity of the pork brain and sterilized, and then preprocessing homogenate after cooling;

[0070] S3, enzymatic solution: Add a protease in the pretreatment homogenate, the adjustment system pH is 7.0, the temperature is 58 ° C, and the temperature is stirred under temperature under this conditions, and the enzymatic hydrolyzate is obtained; wherein the protease is a papain protease and trypsin, papaya The weight ratio of protease and pig brain is 0.3: 100, the weight ratio of trypsin and the pig brain is 0.4: 100;

[00...

Embodiment 2

[0078] The difference from the first embodiment is that the protease in S3 is a papain protease and trypsin, the weight ratio of papain protease and the pig brain is 0.5: 100, the weight ratio of trypsin and the pig brain is 0.3: 100.

Technical field

[0001] The present application relates to the field of food processing, and more specifically, it relates to cerebral protein hydrolyzatics and preparation methods thereof and compositions comprising brain hydrolyzate. Background technique

[0002] Ethylene protein hydrolyza refers to a mixture of hydrolyzed, protein, amino acid, and the like from animal brain tissue, which can act on human neurotic tissue, can improve nerve cell metabolism, promote protein synthesis of neural cells, and protect nerves. Cells are from ischemia and neurotoxin damage, with the role of improving symptoms such as insomnia, headache, memory loss, dizziness and irritability.

[0003] In the relevant technique, the preparation method of brain hydrolyza usually includes the following steps: animal brain tissue → pre-treatment → homogenate → enzymatic hydrolysis → centrifugal → ultrafiltration → column chromatography → sterilization. Due to high fat and cholesterol in the animal brain (suc...

Examples

preparation example Construction

[0053] The present application provides a method of preparing an brain hydrolyzate, including the following steps:

[0054] S1, tissue homogenization: After cleaning the animal brain, remove the meningum and blood vessels, rinse the surface with deionized water, add 2-3 times of deionized water by weight of the animal brain, and then rubbed twice with colloidal grinding. Extremely spray;

[0055] S2, passivation enzyme activity: After boiling the homogenate, maintain 20-40 min, to passivate the enzyme activity and sterilize the animal brain tissue, and then cool the pretreative homogenate;

[0056] S3, enzymatic hydrolysis: The pretreatment homogenate is added to the protease, the adjustment system pH is 6.5-7.5, and the temperature is 55-60 ° C, and the temperature is stirred under temperature under this condition, hydrolyzing hydrolysis is 6-12 h, resulting in an enzymatic hydrolyzide; wherein the protease is papaya. The weight ratio of protease and trypsin and ponybain and anim...

Embodiment 1

[0067] Example 1: A method of preparing an brain hydrolyzate comprising the steps of:

[0068] S1, tissue homogenate: After cleaning fresh pig brain, remove the meningum and blood vessels, flush the surface with deionized water, add 2 times deionized water in the weight of the pig brain, and then rub it twice with colloidal grinding. Homogenate;

[0069] S2, passivation enzyme activity: After boiling the homogenate, maintained for 30 min to passivate the enzyme activity of the pork brain and sterilized, and then preprocessing homogenate after cooling;

[0070] S3, enzymatic solution: Add a protease in the pretreatment homogenate, the adjustment system pH is 7.0, the temperature is 58 ° C, and the temperature is stirred under temperature under this conditions, and the enzymatic hydrolyzate is obtained; wherein the protease is a papain protease and trypsin, papaya The weight ratio of protease and pig brain is 0.3: 100, the weight ratio of trypsin and the pig brain is 0.4: 100;

[00...

Embodiment 2

[0078] The difference from the first embodiment is that the protease in S3 is a papain protease and trypsin, the weight ratio of papain protease and the pig brain is 0.5: 100, the weight ratio of trypsin and the pig brain is 0.3: 100.

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