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Nanometer material capable of efficiently removing cfDNA and ROS as well as preparation method and application of nanometer material

A nanomaterial and high-efficiency technology, applied in the field of high-efficiency removal of cfDNA and ROS nanomaterials and their preparation, can solve problems such as inflammatory bowel disease that have not yet been seen, and achieve efficient and safe inflammatory bowel disease treatment, good biological safety, good therapeutic effect

Pending Publication Date: 2022-03-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the treatment of inflammatory bowel disease by directly removing cfDNA and ROS and other dangerous molecules through nanomaterials

Method used

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  • Nanometer material capable of efficiently removing cfDNA and ROS as well as preparation method and application of nanometer material
  • Nanometer material capable of efficiently removing cfDNA and ROS as well as preparation method and application of nanometer material
  • Nanometer material capable of efficiently removing cfDNA and ROS as well as preparation method and application of nanometer material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: Preparation of nanomaterials that can efficiently remove cfDNA and ROS

[0081] A preparation method capable of efficiently removing cfDNA and ROS nanomaterials, comprising the steps of:

[0082] (1) Preparation of bis[3-(triethoxysilyl)propyl]diselenide

[0083] Add 10 g of γ-chloropropyltrimethoxysilane dropwise to 30 mL of sodium diselenide solution (wherein the sodium diselenide solution contains 0.0255 mol of sodium diselenide), stir at room temperature (25° C.) overnight (10 hours), Add ice water to stop the reaction, extract with dichloromethane, and dry the organic layer over anhydrous sodium sulfate. The crude product is chromatographed on a silica gel column (300-400 mesh) (petroleum ether (PE): dichloromethane (DCM) = 10-1: 1) The purified dark yellow liquid is bis[3-(triethoxysilyl)propyl]diselenide;

[0084] (2) Preparation of nanomaterials that can efficiently scavenge ROS

[0085] Take 0.6g cationic template (hexadecyltrimethyl-p-toluenesulf...

Embodiment 2

[0089] Embodiment 2: Can efficiently remove cfDNA and ROS nanomaterial (MSN-PEI) to remove the situation of cfDNA and ROS

[0090] The highly efficient removal of cfDNA and ROS nanomaterials (MSN-PEI) prepared in Example 1 contained 100 μM H 2 o 2 The simulated body fluid (the simulated body fluid was purchased from Nanjing Yixun Biotechnology Co., Ltd.) was incubated for 1 day, and the cfDNA and ROS nanomaterials that can efficiently remove cfDNA and ROS after incubation were observed by transmission electron microscopy.

[0091] The results of transmission electron microscopy showed that the incubated MSN-PEI had ROS scavenging ability, and the results were as follows: figure 2 A, 2B.

[0092] The ability of the highly efficient ROS-scavenging nanomaterial (MSN), branched polyethyleneimine (PEI) and MSN-PEI prepared in Example 1 to bind to calf thymus DNA (ct-DNA) was evaluated. Mix ct-DNA solution (25 μL, 10 μg / mL, CAS number: 91080-16-9), PicoGreen (25 μL) and MilliQ w...

Embodiment 3

[0094] Example 3: Efficient removal of cfDNA and ROS nanomaterials for anti-inflammatory and anti-oxidative damage at the cellular level

[0095] Evaluate the anti-inflammatory and anti-oxidative damage of the cfDNA and ROS nanomaterial (MSN-PEI) prepared in Example 1 at the cellular level. RAW 264.7 macrophages were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured at 37 °C in DMEM containing 10% FBS, 1 mM sodium pyruvate and 1% penicillin-streptomycin in a humid environment (with 5% CO 2 )nourish.

[0096] RAW 264.7 macrophages in 2 × 10 4 Cells / well were seeded in a 96-well plate, and LPS with a final concentration of 1 μg / mL was added to incubate for 30 min to obtain activated RAW 264.7 mouse macrophages. Then, highly efficient ROS-scavenging nanomaterials (MSN, final concentration 10 μg / mL), branched polyethyleneimine (PEI, final concentration 10 μg / mL) and efficient cfDNA and ROS-scavenging nanomaterials (MSN-PEI, final concentratio...

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Abstract

The invention discloses a nano material capable of efficiently removing cfDNA and ROS, and belongs to the field of pharmaceutical preparations. The preparation method of the nano material capable of efficiently removing cfDNA and ROS comprises the following steps: (1) preparing bis [3-(triethoxysilyl) propyl] double selenide; (2) preparation of a nano material capable of efficiently removing ROS; and (3) preparing a nano material capable of efficiently removing cfDNA and ROS. The prepared nano material capable of efficiently removing cfDNA and ROS is the first nano material capable of removing cfDNA and ROS at the same time, the nano material capable of efficiently removing cfDNA and ROS is used for loading anti-inflammatory drugs, and the effectiveness and safety of treatment of inflammatory bowel diseases can be effectively improved.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a nanomaterial capable of efficiently removing cfDNA and ROS, a preparation method and application thereof. Background technique [0002] Inflammatory bowel disease is a chronic, relapsing gastrointestinal disease caused by intestinal inflammation and injury, mainly including Crohn's disease and ulcerative colitis. At present, the incidence of inflammatory bowel disease in developed countries has reached a plateau, and there is no obvious improvement trend. With the significant changes in the dietary structure, lifestyle and living environment of Chinese nationals, the incidence of IBD (currently 1.8 per 100,000 people) shows a trend of continuous and rapid growth. At present, there is no specific drug in the clinical treatment of inflammatory bowel disease, and salicylic acid, hormones, immunosuppressants and biological agents are still the main treatments...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/00A61K47/04A61K47/34A61K31/606A61K31/655A61K31/52A61K31/519A61P1/00A61P29/00C07F7/18B82Y5/00B82Y40/00C01B33/18
CPCA61K33/00A61K47/02A61K47/34A61K31/606A61K31/655A61K31/52A61K31/519A61K9/0053A61P1/00A61P29/00C07F7/1892B82Y5/00B82Y40/00C01B33/18C01P2004/64C01P2006/16C01P2006/12C01P2004/03C01P2004/04A61K2300/00
Inventor 邵丹佘军军杨超佳娜提史童飞陈方满石承昕
Owner SOUTH CHINA UNIV OF TECH
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