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Chemical modification method for surface of dental implant combined with alveolar bone and application

A technology of dental implants and surface chemistry, applied in the field of biomedicine, can solve the problems of not fundamentally solving the problem of the combination of implants and alveolar bone, the performance of osseointegration is not improved, and the bonding force of the coating is not strong, etc. To achieve the effect of improving osseointegration performance, improving hydrophilicity, and simple method

Active Publication Date: 2022-04-05
陈栋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the most widely used technologies in clinical practice include micro-arc oxidation, sandblasting acid etching, double-acid etching, and hydroxyapatite spraying technology. Since micro-arc oxidation technology has strict requirements on equipment, it is mainly controlled by foreign companies such as NOBEL BIOCARE. The price remains high; sand blasting acid etching and double acid etching technology are widely used, but there are risks such as falling off and loosening; compared with the above three modification technologies, hydroxyapatite spraying technology is cheap, but due to the existence of bone Problems such as absorption and weak coating adhesion are limited in application
In addition to the above technologies that are widely used, there are also treatment technologies such as plasma spraying, but they have not fundamentally solved the problem of improving the combination of the implant and the alveolar bone at the initial stage of implant implantation, that is, the osseointegration performance has not been improved.

Method used

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  • Chemical modification method for surface of dental implant combined with alveolar bone and application
  • Chemical modification method for surface of dental implant combined with alveolar bone and application
  • Chemical modification method for surface of dental implant combined with alveolar bone and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for chemical modification of the surface of the dental implant combined with the alveolar bone provided in this embodiment, the steps are as follows:

[0030] (1) Material preparation: Polish a cylindrical pure titanium sheet (PureTi) with a diameter of 6 mm and a thickness of 3 mm with a gradient rough sandpaper from coarse to fine to mirror brightness (60#, 200#, 600#, 1000#, 2000#), followed by ultrasonic cleaning in an ultrasonic cleaner for 10 minutes with deionized water, absolute ethanol, and deionized water.

[0031] (2) Preparation of hydrofluoric acid / nitric acid mixed etching solution: pipette 2ml of 40% hydrofluoric acid and 4ml of concentrated nitric acid into 40ml of deionized water, place on a magnetic stirrer and mix evenly.

[0032] (3) Preparation of hydrochloric acid / sulfuric acid mixed acid etching solution: pipette 1ml of concentrated hydrochloric acid and 1ml of concentrated sulfuric acid into 40ml of deionized water, place on a magnetic...

Embodiment 2

[0042] Cell Adhesion Test

[0043] (1) Silane in Example 1 that had been sterilized was used as the experimental group, PureTi, SLA and UV were used as the control group, and 3 slices were taken from each group as parallel samples, and put into a 48-well plate, 1 slice per hole.

[0044] (2) The 2nd-4th generation rat bone marrow mesenchymal stem cells (BMSCs) were mixed with 5×10 4 / ml cell density seeded on the surface of the titanium sheet, placed at 37 ° C, 5% CO 2 cultured in an incubator.

[0045] (3) 2h, 4h, 12h and 24h after cell inoculation, take out the 48-well plate, remove the original culture medium, and wash with phosphate buffer saline (PBS).

[0046] (4) Add 4% paraformaldehyde to each well and fix at room temperature for 10 min.

[0047] (5) Remove paraformaldehyde in the wells, wash with PBS, and wash 2-4 times with PBS containing 0.1% Triton X-100 at room temperature, about 5 minutes each time.

[0048] (6) Dilute Actin-Tracker Green with PBS containing ...

Embodiment 3

[0054] Cell Viability and Proliferation Assays

[0055] (1) CCK-8 detection:

[0056] (1) The sterilized Silane in Example 1 was used as the experimental group, and PureTi, SLA, and UV were used as the control group. Each group took 3 slices as parallel samples and put them into a 48-well plate, with 1 slice per hole.

[0057] (2) The 2nd-4th generation rat bone marrow mesenchymal stem cells (BMSCs) were mixed with 5×10 4 / ml cell density seeded on the surface of the titanium sheet, placed at 37 ° C, 5% CO 2 cultured in an incubator.

[0058] (3) Prepare CCK-8 solution according to the ratio of CCK-8: complete medium = 1:10.

[0059] (4) Take out the 48-well plate on, 3, 5, and 7 days respectively, remove the original culture medium, and wash with PBS.

[0060] (5) 500 μL of the prepared CCK-8 solution was added to each well, and placed in an incubator for further incubation for 2 hours.

[0061] (6) Take out the well plate, pipette 100 μL per well, transfer to a 96-well ...

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Abstract

The invention discloses a surface chemical modification method and application of a dental implant combined with an alveolar bone, and belongs to the field of biological medicine. The method comprises the following steps: taking titanium or titanium alloy as a dental implant combined with an alveolar bone, carrying out grinding, polishing and double acid etching on the surface of the titanium or titanium alloy to obtain frosted and acid-etched titanium or titanium alloy, and then carrying out ultraviolet light treatment and alkali liquor treatment to obtain the dental implant with a hydrophilic multistage microporous layer on the surface, then, 3-aminopropyltriethoxysilane is subjected to hydrogel treatment and then is spin-coated on the surface of the dental implant subjected to surface treatment, and the 3-aminopropyltriethoxysilane self-assembly layer is formed. Through the chemical modification method, the dental implant and an alveolar bone bonding interface are effectively reacted, the bonding rate of bone cells and the titanium or titanium alloy dental implant can be increased, the dental implant is used for hard tissue repair, the clinical success rate is increased, and the shedding rate in a dental operation is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a surface chemical modification method and application of a dental implant combined with alveolar bone. Background technique [0002] since The professor proposed the concept of osseointegration in 1952 and developed the first titanium implant in 1964. Titanium and its alloys have gradually become the orthopedic implants because of their excellent biocompatibility, chemical corrosion resistance and comprehensive mechanical properties. Especially the material of choice for human hard tissue substitutes and restorations in dentistry, but the material still suffers from implant failure. Long-term clinical and research studies have found that the main reason for implant failure at this stage is that the biological activity of the implant is not ideal, resulting in poor osseointegration ability of the hard tissue implant, poor integration with surrounding tissues, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/06A61L27/28A61L27/50A61L27/56A61C8/00
Inventor 梁子骐陈栋黄光斌
Owner 陈栋
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