Polyethylene glycol modified graphene oxide as well as preparation method and application thereof

A polyethylene glycol modification, polyethylene glycol technology, applied in the field of polyethylene glycol modified graphene oxide and its preparation, can solve the severe reaction process of potassium permanganate and concentrated sulfuric acid, and the limited scope of application of materials It is easy to store and transport, has good application potential, and is easy to separate.

Pending Publication Date: 2022-04-05
国科广化韶关新材料研究院 +2
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the preparation process of graphene oxide uses the hummer method, the reaction process between potassium permanganate and concentrated sulfuric acid is violent, which may easily lead to safety accidents.
Moreover, there are a large number of amine groups and carboxyl groups on the surface of the prepared graphene oxide, which makes the surface have a large amount of charge, which may denature the protein adsorbed on the surface.
Chinese patent ZL 201310756622.2 discloses a pretreatment method to improve protein purity, using graphene and metal, semiconductor or polymer nanoparticle composites, due to the graphene itself and the nanoparticles on the surface, it may cause poor dispersion in water , and easy to settle after adsorbing protein
At present, there have been many patent reports on polyethylene glycol-modified graphene, but most of the modification methods are based on the esterification / amide between polyethylene glycol / aminated polyethylene glycol and the carboxyl group at the edge of the graphene oxide sheet chemical reaction (such as CN201210031478.1, CN201210004010.3, CN201510202859.5) or carry out grafting reaction on the surface of graphene oxide by adding "bridge" substances such as cross-linking agent (such as CN201711094213.5, CN201810319573.9), and change Polyethylene glycol is mostly concentrated on the edge of the graphene oxide sheet, and the exposed surface of the unmodified graphene oxide is prone to stacking and agglomeration in the solution environment, which will have a negative impact on the performance of the adsorption material.
At the same time, since the ester bond / amide bond is easily hydrolyzed and broken under alkaline conditions, this also limits the scope of application of the modified material.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polyethylene glycol modified graphene oxide as well as preparation method and application thereof
  • Polyethylene glycol modified graphene oxide as well as preparation method and application thereof
  • Polyethylene glycol modified graphene oxide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A kind of preparation of polyethylene glycol modified graphene oxide, comprises the following steps:

[0055] (1) Fully mix 0.1g of graphene oxide, 10g of sulfur trioxide and 500mL of deionized water, ultrasonically disperse for 3 hours, heat to 50°C, and react for 5 hours to obtain a graphene oxide precursor;

[0056] (2) Mix 0.1 g of the product of step (1) with 2 g of allyl polyoxyethylene ether (Mn=1000), 0.1 g of calcium chloride, 0.002 g of zinc chloride, and 40 g of dimethyl sulfoxide, and disperse by ultrasonic 2h, heated to 50°C, and reacted for 24h to obtain polyethylene glycol-modified graphene oxide, labeled as GO-1.

Embodiment 2

[0058] A kind of preparation of polyethylene glycol modified graphene oxide, comprises the following steps:

[0059] (1) Fully mix 0.1g of graphene oxide, 10g of chlorosulfonic acid and 500mL of deionized water, ultrasonically disperse for 3 hours, heat to 50°C, and react for 5 hours to obtain a graphene oxide precursor;

[0060] (2) Fully mix 0.1 g of the product of step (1) with 5 g of polyethylene glycol (Mn=4000), 5 g of polyphosphoric acid, 0.01 g of cuprous chloride, and 36 g of butanone, ultrasonically disperse for 20 min, and heat for 36 h. The reaction temperature was 25°C, and polyethylene glycol-modified graphene oxide was obtained, labeled as GO-2.

Embodiment 3

[0062] A kind of preparation of polyethylene glycol modified graphene oxide, comprises the following steps:

[0063] (1) Fully mix 0.1 g of reduced graphene oxide and 30 g of concentrated sulfuric acid, ultrasonically disperse for 30 minutes, and heat to 25°C for 30 minutes to obtain a graphene oxide precursor;

[0064] (2) Fully mix 0.1g of the product of step (1) with 6g of allyl polyoxyethylene ether (Mn=1000), 2g of calcium oxide, 0.001g of tetramethylethylenediamine, and 100g of acetone, ultrasonically disperse for 2h, and heat After reacting for 8 hours at a reaction temperature of 50°C, polyethylene glycol-modified graphene oxide was obtained, which was labeled as GO-3.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
adsorption capacityaaaaaaaaaa
adsorption capacityaaaaaaaaaa
Login to View More

Abstract

The invention discloses polyethylene glycol modified graphene oxide as well as a preparation method and application thereof. The preparation method of the polyethylene glycol modified graphene oxide comprises the following steps: reacting a carbon-containing solid with a reactant in a solvent to obtain a graphene oxide precursor, dispersing the graphene oxide precursor, a polyethylene glycol substance and a dehydrating agent in an aprotic solvent, and carrying out a catalytic reaction with a catalyst to obtain the polyethylene glycol modified graphene oxide. According to the polyethylene glycol modified graphene oxide disclosed by the invention, agglomeration of graphene oxide is reduced, and the polyethylene glycol modified graphene oxide has good dispersity; the method has the characteristics of good sensitivity, high adsorption capacity and good stability, can exert good performance in acid-base environments of most biological samples, is more suitable for extraction and separation of low-abundance proteins in complex biological samples, can effectively remove protein pollution in the biological samples, and has application potential in sample pretreatment in the fields of biology and medicine.

Description

technical field [0001] The invention relates to the field of polymer materials, in particular to a polyethylene glycol-modified graphene oxide and its preparation method and application. Background technique [0002] The separation of high-purity protein samples is of great significance in the fields of biology and medicine. The proportion of high-abundance proteins in the proteins obtained by ordinary adsorption separation methods is too large, and some low-abundance proteins cannot be separated, which leads to the subsequent analysis of proteomics. More and more accurate protein information cannot be obtained. In order to separate and obtain low-abundance proteins, the commonly used method is immunoaffinity column chromatography, which binds antibodies to specific proteins (high-abundance proteins) to separate unbound serum proteins, and finally achieves the removal of high-abundance proteins. protein purpose. However, this method is expensive, and the immunoaffinity col...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityApplications(China)
IPC IPC(8): B01J20/26B01J20/30C07K1/14C07K14/765C07K14/805G01N21/31
CPCY02E60/10
Inventor王斌周珊珊黄月文
Owner国科广化韶关新材料研究院