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Heparin sodium refined product as well as extraction method and application thereof

An extraction method and heparin sodium technology, which are used in cosmetics, packaged food, drug packaging, etc., can solve problems such as inability to remove prions, achieve large market application value, reduce the probability of possible disease, and reduce the probability of adverse reactions in the human body Effect

Pending Publication Date: 2022-04-05
上海帕尼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defect that prions cannot be removed by bovine or sheep heparin sodium extraction methods in the prior art, the present invention provides a high-quality heparin sodium and its extraction method and application

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A kind of heparin sodium high-quality goods extracting method, it comprises the following steps:

[0048] (1) Pretreatment: Take 5000MU of bovine heparin sodium crude product (about 100kg, potency is 50IU / mg), dissolve it in 500L pure water at 10000IU / mL, add 10kg of sodium chloride, and use 5% hydrochloric acid or 20% hydrogen Sodium oxide aqueous solution to adjust the pH to 8; then add 2000kg of FPA98 anion exchange resin, adsorb at 60°C for 3 hours, filter, and collect the adsorption resin; first rinse with tap water, and then use 4% chlorination of 2 times the weight of the adsorption resin After washing with sodium aqueous solution at room temperature, elute with 20% sodium chloride aqueous solution for two times, and combine the eluents; add ethanol to 50% for alcohol precipitation, and obtain precipitate A;

[0049] (2) Acid-base treatment: Precipitate A is dissolved with 500L pure water, adjusted to pH 1.8 with 5% hydrochloric acid and maintained for 0.5 hours,...

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Abstract

The invention discloses a refined heparin sodium product as well as an extraction method and application thereof. The extraction method comprises the following steps: (1) pretreating to obtain a precipitate A; (2) acid and alkali treatment: adjusting the pH value of the aqueous solution of the precipitate A to 1.5-2.0, and filtering to obtain an acidic filtrate; adjusting the pH value of the acidic filtrate to 11-12, stirring at 90-92 DEG C, and filtering to obtain an alkaline filtrate; carrying out wine precipitation on the alkaline filtrate to obtain a precipitate B; (3) oxidation: oxidizing the aqueous solution of the precipitate B for 2-4 times to obtain a precipitate C; and (4) freeze-drying. According to the extraction method, prion can be effectively prevented from entering the refined heparin sodium product, the refined heparin sodium product does not contain prion, the content of other types of protein impurities is low, and the extraction method has great market application value.

Description

technical field [0001] The invention relates to a high-quality heparin sodium and its extraction method and application. Background technique [0002] Heparin sodium is an anticoagulant drug widely used in clinical practice, and its basic raw material is extracted from the visceral tissue of mammals. Commonly used sources are porcine small intestinal mucosa, bovine lung and bovine small intestinal mucosa, and sheep small intestinal mucosa, among which porcine small intestinal mucosa is the most important source of extraction. With the tight supply of heparin sodium in the world and frequent outbreaks of pig diseases, heparin sodium from cattle and sheep has the potential to return to the mainstream market. [0003] Creutzfeldt-Jakob disease (commonly known as mad cow disease, BSE) and sheep scrapie (TSE) have appeared in cattle and sheep in history. The pathogenic source of BSE / TSE is prion (prion protein), which is a protein substance that can replicate itself and make no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10A61K31/727A61P7/02A61K8/73A61Q19/00A61L33/10A61L33/00
Inventor 韩旭阮文兴毛兴昌
Owner 上海帕尼生物科技有限公司