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Method for promoting deep fermentation spore production and sugar production of antrodia camphorata by adding cinnamomum kanehirai extract

A technology of submerged fermentation and camphor tree, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low yield of asexual spores and active substances, high cost, low production efficiency of active substances, etc. , to achieve good spore production effect, increase yield, and promote the effect of spore production

Pending Publication Date: 2022-04-05
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: to solve the problem of low production efficiency and high cost of Antrodia camphorata deep fermentation seeds (asexual spores) preparation and active substance production due to the low yield of antrodia deep fermentation asexual spores and active substances

Method used

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  • Method for promoting deep fermentation spore production and sugar production of antrodia camphorata by adding cinnamomum kanehirai extract
  • Method for promoting deep fermentation spore production and sugar production of antrodia camphorata by adding cinnamomum kanehirai extract
  • Method for promoting deep fermentation spore production and sugar production of antrodia camphorata by adding cinnamomum kanehirai extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation steps of Cinnamomum camphora aqueous extract are as follows:

[0047] (1) Weigh 100g of camphor wood chips, add 1L of deionized water, and boil for 30min;

[0048] (2) filter with 2 layers of gauze, collect the filtrate and concentrate 10 times the volume with a rotary evaporator;

[0049] (3) The concentrated filtrate is vacuum freeze-dried to obtain the water extract powder of Cinnamomum camphora;

[0050] (4) Redissolve the dried Cinnamomum camphora aqueous extract powder with water, prepare a mother liquor with a concentration of 10 mg / mL and filter and sterilize with a filter membrane with a diameter of 0.45 μm;

[0051] (5) Divide the mother liquor of the sterilized Cinnamomum camphora water extract and store it in a -20°C refrigerator for later use.

Embodiment 2

[0053] The preparation steps of camphor tree methanol extract are as follows:

[0054] (1) Weigh 100g of camphor wood chips, add 1L of methanol, shake and extract at 300r / min for 2h;

[0055] (2) filter with 2 layers of gauze, collect the filtrate and concentrate 10 times the volume with a rotary evaporator;

[0056] (3) Place the concentrated filtrate in an oven at 65°C and dry to constant weight to obtain the methanol extract powder of Cinnamomum camphora;

[0057] (4) Reconstitute the dried Cinnamomum camphora methanol extract powder with water, prepare a mother liquor with a concentration of 10 mg / mL and filter and sterilize it with a filter membrane with a diameter of 0.45 μm;

[0058] (5) Divide the sterilized mother liquor of the methanol extract of Cinnamomum camphora and store it in a -20°C refrigerator for later use.

Embodiment 3

[0060] The preparation steps of Cinnamomum camphora ethyl acetate extract are as follows:

[0061] (1) Weigh 100g of camphor wood chips, add 1L of ethyl acetate, shake and extract at 300r / min for 2h;

[0062] (2) filter with 2 layers of gauze, collect the filtrate and concentrate 10 times the volume with a rotary evaporator;

[0063] (3) Place the concentrated filtrate in an oven at 85°C and dry to constant weight to obtain the ethyl acetate extract powder of camphor tree;

[0064] (4) Reconstitute the dried Cinnamomum camphora ethyl acetate extract powder with water, prepare a mother liquor with a concentration of 10 mg / mL, and filter and sterilize with a filter membrane with a diameter of 0.45 μm;

[0065] (5) Divide the sterilized mother liquor of the ethyl acetate extract of Cinnamomum camphora and store it in a -20°C refrigerator for later use.

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Abstract

The invention discloses a method for promoting deep fermentation spore production and sugar production of antrodia camphorata by adding a cinnamomum kanehirai extract. The method comprises the following steps: adding a trace amount of cinnamomum kanehirai aqueous extract into an antrodia camphorata submerged fermentation culture medium, and then inoculating antrodia camphorata for fermentation. Compared with a control group without the Cinnamomum kanehirai aqueous extract, the method provided by the invention can increase the yield of asexual spores of deep fermentation of Antrodia camphorata by more than 50%, increase the biomass by more than 20% and increase the content of intracellular polysaccharides in mycelia by more than 100%, and the effect is very remarkable. The method can be used for preparing a large number of seeds (antrodia asexual spores) in the industrial production process of antrodia, meanwhile, the intracellular polysaccharide content of antrodia mycelia generated in the seed preparation process is remarkably increased, the production efficiency and benefits are remarkably improved, and the production cost is reduced. In addition, the Cinnamomum kanehirae aqueous extract used in the invention has the advantages of extremely low addition concentration, wide source, high safety, environmental friendliness, and great development value and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for promoting the spore production and sugar production of antrodia camphorata by adding camphor tree extract. Background technique [0002] Antrodia cinnamomea, also known as Antrodia cinnamomea or Antrodia cinnamomea, is a rare medicinal and edible fungus, known as "forest ruby". Antrodia camphorata is often used to treat hangover, food poisoning, abdominal pain, diarrhea, inflammation and skin itching, etc., and the effect is remarkable. At present, scientific researchers have confirmed that Antrodia camphorata has many functions such as hangover protection, anti-tumor, anti-cancer, anti-virus, anti-inflammation, anti-oxidation, anti-fatigue and immune regulation. active substances, including polysaccharides, diterpenoids, triterpenoids, steroids and benzene ring derivatives, etc. Among them, maleic acid and succinic acid derivatives (Antro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14C12N3/00C12P19/04C12R1/645
Inventor 李华祥王娟娟石瑀杨振泉饶胜其高亚军袁磊郑香峰周文渊关天竺
Owner YANGZHOU UNIV
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