Method for preventing and controlling pear ring spot by using dopamine
A technology of pear ring spot and dopamine, which is applied in the field of fruit tree disease prevention and control, to achieve the effects of convenient use, cost saving, and reduction of pesticide use
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Embodiment 1
[0051] Dopamine under different concentrations of embodiment 1 prevents and controls pear ring spot
[0052] Set 50, 100, 200, 400μM four concentrations of dopamine aqueous solution, spray 30 tablets of each of the four concentrations of dopamine aqueous solution on the harvested 'Dangshan' pear leaves with clear water treatment as a control, and inoculate the ringworm after 24 hours of treatment. The diameter of the lesion was counted every two days.
[0053] Indoor spraying of different concentrations of dopamine test results are as follows: figure 1 As shown, 50, 100, 200, 400μM four concentrations of dopamine treatment can inhibit the growth of pear ring disease, and the diameter of the lesion decreased by 41.6%, 71.6%, 54%, 62.3%, respectively. Among them, 100 μM dopamine has the best treatment effect, so subsequent experiments all use this concentration for treatment.
Embodiment 2
[0055] Three plants of 'Cuiguan', 'Sucui No.1' and 'Yuluxiang' with the same tree vigor, tree age and load capacity were selected for the test. Spray 100 μM dopamine solution and add 1% Tween80 every 15-20 days during the period of fruit expansion. The spraying time of 'Su Cui No. 1' is (May 1, May 21, June 11, July 1, 2020) and the spraying time of 'Cuiguan' is ( May 1st, May 21st, June 11th, July 1st, July 21st, 2020) were sprayed 5 times in total, and the spraying time of 'Yuluxiang' was (May 1st, 2020, May 21st, June 11th, July 1st, July 21st, August 10th, September 1st, September 20th) sprayed 8 times in total. The spraying time is 8-9 o'clock in the morning when the weather is fine, or 4-5 o'clock in the afternoon, and spraying with clear water is used as a control test.
[0056] Select pear fruits with the same maturity and size without mechanical damage and pests, wash the surface with sterile water, pierce the middle of the fruit with a sterilized needle, pierce a h...
Embodiment 3
[0059] Three 'Dangshan' pears with the same tree vigor, tree age and load were selected for the experiment.
[0060] The pear leaves were punctured at the upper and lower positions on the left and right sides, inoculated with the pathogenic bacteria of ring print, cultured at 25°C, and the diameter of the lesion was measured at 0, 2, 4, 6, and 8 days respectively. Dangshan’ pear leaves were treated with 100 μM dopamine and inoculated with the pathogenic bacteria Figure 8 The lesion diameters showed that the average lesion diameters on days 2, 4, 6, and 8 were significantly lower than those treated with CK, and decreased by 62.2%, 37.3%, 29.1%, and 26.1%, respectively.
[0061] The activities of phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), peroxidase (CAT), peroxidase (POD), Hydrogen peroxide (H 2 o 2 ) content and superoxide anion (OFR) content. Enzyme activity, hydrogen peroxide and superoxide anion content test results reference Figure 9-13 , as show...
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