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Bacillus coagulans, application thereof, composition and fermentation culture method of bacillus coagulans

A technology of Bacillus coagulans and fermentation medium, which is applied in the field of Bacillus coagulans and its application, composition and fermentation culture of Bacillus coagulans, which can solve the problems of lower quality, low spore production rate, and high bacterial contamination rate.

Active Publication Date: 2022-04-12
WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the current fermentation production of Bacillus coagulans has the disadvantages of high bacterial contamination rate, low sporulation rate, and low bacterial count, which is not conducive to industrial production and reduces the quality of products containing Bacillus coagulans. The fermentation culture method of bacillus is of great value to improve the fermentation level of Bacillus coagulans, especially to improve the transsporulation rate of Bacillus coagulans

Method used

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  • Bacillus coagulans, application thereof, composition and fermentation culture method of bacillus coagulans
  • Bacillus coagulans, application thereof, composition and fermentation culture method of bacillus coagulans
  • Bacillus coagulans, application thereof, composition and fermentation culture method of bacillus coagulans

Examples

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Embodiment 1

[0101] This embodiment provides a method for fermentation and cultivation of Bacillus coagulans NJ, wherein the fermentation scale is a 2-ton fermenter.

[0102] 1. Preparation of seed solution

[0103] (1) Seed activation

[0104] Take the Bacillus coagulans NJ stored in the glycerol tube at -80°C. After the Bacillus coagulans NJ glycerol tube is completely melted, draw 1.0mL of the bacterial liquid and inoculate it into 50mL of MRS liquid medium, and place it under the conditions of 45°C and 250r / min Shake the flask and culture for 15 hours, then pick the bacterial solution and streak it on the MRS medium plate, place it at 45°C for anaerobic culture for 24 hours, and obtain a single colony of Bacillus coagulans NJ.

[0105] (2) Preparation of primary seed solution

[0106] Take a single colony of Bacillus coagulans NJ grown on the MRS medium plate, disperse it in 2mL of normal saline, then inoculate it in 50mL of MRS liquid medium, and place it under the conditions of 45°...

Embodiment 2

[0123] This embodiment provides a method for fermentation and cultivation of Bacillus coagulans NJ, wherein the fermentation scale is a 30L fermenter. The specific operation process is: according to the method in embodiment 1, prepare the primary seed liquid and the secondary seed liquid, and inoculate the secondary seed liquid into the fermenter of 30L according to the inoculum size of 1% by volume (the liquid capacity is 16L, the fermentation medium is the same as in Example 1, and the actual elimination method of the fermenter is carried out with reference to Example 1), the initial aeration ratio is 0.5vvm, the tank pressure is maintained at 0.05Mpa, the temperature is controlled at 47°C, the minimum stirring speed is started to ferment, and the feeding 50% phosphoric acid is 5.9 (consumption situation of phosphoric acid in the fermentation process is as follows to control fermentation pH Figure 11 Shown), the whole fermentation process maintains dissolved oxygen not less...

Embodiment 3

[0126] This embodiment provides a method for fermentation and cultivation of Bacillus coagulans NJ, wherein the fermentation scale is a 2-ton fermenter. Compared with the fermentation process of Example 1, the difference of the fermentation process of this embodiment is that the fermentation medium is different.

[0127] The fermentation medium of this embodiment is: 25g / L yeast extract powder, 25g / L peptone, 5g / L beef extract, 8g / L fermented soybean meal, 5g / L sodium acetate, 2g / L hydrogen phosphate Dipotassium, 0.5g / L magnesium sulfate, 0.5g / L calcium chloride, 0.2g / L manganese sulfate and 0.2g / L iron sulfate.

[0128]In this example, the fermented liquid in the lower tank was diluted and spread on the MRS medium plate, and three parallel samples were set for each dilution, and counted after static culture at 40°C for 24 hours. By counting, the bacteria in the fermented liquid in the lower tank could be known The body concentration is 8 billion CFU / mL, and the spore concent...

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Abstract

The invention discloses bacillus coagulans, an application of the bacillus coagulans, a composition of the bacillus coagulans and a fermentation culture method of the bacillus coagulans. The name of the bacillus coagulans is NJ, the classification name of the bacillus coagulans is Bacillus coagulans, the preservation number of the bacillus coagulans is CCTCC NO: M 20211445, the preservation date of the bacillus coagulans is November 18, 2021, and the preservation unit of the bacillus coagulans is China Center for Type Culture Collection. The active component of the composition comprises bacillus coagulans NJ, and the composition is a feed additive, an animal feed, a food additive, food or a probiotic agent. According to the fermentation process of the bacillus coagulans, the bacillus coagulans is inoculated into a novel fermentation culture medium for fermentation culture under the conditions that the temperature is 45-50 DEG C, the pH is 5.5-6.0 and the dissolved oxygen is not lower than 20%, and the bacillus coagulans has the advantages of being high in sporulation rate, high in thallus concentration and low in contamination rate.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a bacillus coagulans, its application, a composition and a method for fermenting and cultivating the bacillus coagulans. Background technique [0002] Bacillus coagulans (Bacillus coagulans) belongs to the genus Bacillus. Bacillus coagulans is Gram-positive, can move, and can form endospores. Bacillus coagulans can grow in both aerobic and anaerobic environments, but the amount of lactic acid produced by fermentation under anaerobic conditions is significantly higher than that produced by fermentation under aerobic conditions. Bacillus coagulans has both the lactic acid producing characteristics of lactic acid bacteria and the stress resistance characteristics of Bacillus coagulans, making Bacillus coagulans a unique spore probiotic, widely used in medicine, food, animal husbandry and aquaculture and other fields. [0003] Bacillus coagulans has a powerful enzyme ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30A23K50/75A23K50/10A23L33/135C12R1/07
Inventor 金梅林钟鸣杨欢欢缪鑫昕姚燕妮柯土旺张强
Owner WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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