Photo-crosslinking molecular probe fixed on special slide as well as preparation method and application of photo-crosslinking molecular probe

Abstract

The invention discloses a photo-crosslinking molecular probe fixed on a special slide as well as a preparation method and application of the photo-crosslinking molecular probe, and relates to the technical field related to screening of active ingredients of traditional Chinese medicines. The photo-crosslinking molecular probe takes trifluoromethyl phenyl bis (aziridine) as a photo-crosslinking group, is fixed on an amino glass slide through an oxygen-containing straight-chain connecting bridge, and can be combined with various traditional Chinese medicine small molecules under the irradiation of 365nm ultraviolet light to construct the photo-crosslinking molecular probe. The distance between the photo-crosslinking group and the carrier is prolonged through the oxygen-containing straight-chain connecting bridge, the probability of specific binding of the active small molecules and the target protein is increased, the active small molecules are fixed to the special carrier through the photoaffinity connecting chain, and further confirmation analysis of the'fished 'target protein can be conveniently achieved. The photo-crosslinking reaction is carried out in a non-selective mode, and the method is suitable for traditional Chinese medicine and compound systems with complex and diversified components, is applied to the field of traditional Chinese medicine active component screening, and has the advantages of being high in flux, high in speed, high in timeliness and easy to implement.

Description

technical field [0001] The invention belongs to the technical field related to the screening of pharmaceutical active ingredients, and in particular relates to a photocrosslinked molecular probe fixed on a special glass slide and a preparation method and application thereof. Background technique [0002] Molecular probe technology is a target research technology developed from the perspective of chemical biology. It plays a key role in both activity-based protein profiling and compound-centered chemical proteomics. By using activity-oriented probe molecules Specifically label target proteins in complex biological samples to study the interaction of small molecules with target proteins. As a fast, simple and time-controllable cross-linking tool, photocross-linking reaction is widely used in many research fields such as chemistry, biology, medicine and materials. The synthesized photosensitive small molecular compound is used as a tool probe to Under light irradiation, a high...

AI Technical Summary

Problems solved by technology

Photocrosslinking molecular probes are generally composed of active molecules, photocrosslinking groups, linking sites, and reporter groups, but there are many limitations in application: for natural small molecules with complex structures, chemical modification is difficult and cannot be fully performed. structure-activity relationship research; chemically labeled small molecules may bind to target proteins due to changes in chemical properties and cause false positive results; the constructed molecular probes are only used for the screening of single-component targets, and for traditional Chinese medicines with diverse chemical components It requires a huge workload; at the same time, the molecular probe in the free state is difficult to track after binding to the target protein, and the separation and identification steps are complicated and cumbersome.

Images