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Bacillus atrophaeus and application of bacillus atrophaeus in prevention and control of Chinese wolfberry diseases

A technology of Bacillus rysophila and Bacillus, which is applied in the field of Bacillus fuencus and in the prevention and control of diseases of wolfberry, can solve the problems of severe drug resistance, achieve a wide antibacterial spectrum, do not pollute the environment, and are not easy to produce drug resistance Effect

Pending Publication Date: 2022-04-15
HUBEI BIOPESTICIDE ENG RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical fungicides are mainly used for the prevention and control of the two diseases, and hymexazol and carbendazim are mainly used for root irrigating in the production of root rot. According to the query of China Pesticide Information Network, the registered drug for wolfberry powdery mildew is mainly difenoconazole , pyraclostrobin and tebuconazole, due to the frequent use of chemical fungicides, resistance is increasing

Method used

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  • Bacillus atrophaeus and application of bacillus atrophaeus in prevention and control of Chinese wolfberry diseases
  • Bacillus atrophaeus and application of bacillus atrophaeus in prevention and control of Chinese wolfberry diseases
  • Bacillus atrophaeus and application of bacillus atrophaeus in prevention and control of Chinese wolfberry diseases

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Acquisition and identification of Bacillus fuencus:

[0026] An isolated bacterium, isolated from the rhizosphere soil of Lycium barbarum, was identified as Bacillus atrophaeus ( figure 1 , Table 1), has been sent to the China Center for Type Culture Collection on October 18, 2021 for preservation, classification and name: Bacillus atrophaeus (Bacillus atrophaeus) GQ260; preservation number is CCTCC NO: M 20211286; place: China, Wuhan, Wuhan University.

[0027] In the present invention, Bacillus fumeus GQ260 may be referred to as GQ260 for short.

[0028] Morphological characteristics: the colony is round, milky white.

[0029] Physiological and biochemical characteristics of the strain:

[0030] Table 1 Physiological and biochemical characteristics of strain GQ260 – carbon source utilization

[0031]

[0032]

[0033]

[0034] +: Positive reaction; -: Negative reaction; W: Weak positive reaction

Embodiment 2

[0036] Fermentation of Bacillus Fuesus GQ260 (percentages in the following medium formula are mass fractions):

[0037] 1) First-level seed culture: 100ml of first-level seed medium is placed in a 500ml Erlenmeyer flask, sterilized by damp heat at 121°C for 20 minutes, and the strains from GQ260 freeze-dried tubes are inoculated into the first-level seed medium, and shaken at 35°C and 180rpm Shaking culture for 10 hours; the raw materials and dosage of the medium used for primary seed cultivation are: 1% glucose, 2% beef extract, 3% peptone, 0.5% sodium chloride, and the pH of the medium is 7;

[0038] 2) Secondary seed culture: 300L secondary seed medium is installed in a 500L fermenter, sterilized by moist heat at 121°C for 20 minutes, and 200ml of primary seed is inoculated into the secondary seed medium; cultured at 35°C for 10h; tank pressure is controlled 0.05Mpa, stirring speed 200rpm, aeration ratio 1:1, the raw materials and dosage of the medium used for secondary see...

Embodiment 3

[0042] Bacteriostatic activity of Bacillus fumarus GQ260 and its control effect on root rot and powdery mildew of Lycium barbarum

[0043] Fusarium solani, the pathogenic fungus of wolfberry root rot, was isolated from diseased wolfberry strains and stored on PDA slopes at 4°C, and other pathogenic fungi were stored on PDA slopes at 4°C. The slant strain was transferred to a PDA plate for activation, and cultured at 30°C for 6 days for later use. Place sterilized filter paper sheets at both ends of the PDA plate, and then drop 7 μL of GQ260 fermentation broth. After 24 hours, use a 4mm hole puncher to insert the above-mentioned pathogenic bacteria plate, and use sterile water as a blank control, and the distance between the pathogenic bacteria and the filter paper is 25mm. After the plate with the control was overgrown, the diameters of the colonies of each treatment and the control were counted, and the bacteriostatic rate was calculated.

[0044] Bacterial inhibition rate=...

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Abstract

The invention belongs to the technical field of crop prevention and control, and particularly relates to bacillus atrophaeus and application of the bacillus atrophaeus in prevention and control of Chinese wolfberry diseases. The bacillus atrophaeus is bacillus atrophaeus GQ260, the preservation number of the bacillus atrophaeus GQ260 is CCTCC NO: M20211286, and the strain is reported for the first time and can be used for preventing and controlling powdery mildew of Chinese wolfberry through foliar spraying and preventing and controlling root rot of Chinese wolfberry through root irrigation, and the bacillus atrophaeus has a wide application prospect in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of crop prevention and control, and in particular relates to a strain of bacillus fueci and its application in the prevention and control of wolfberry diseases. Background technique [0002] Lycium barbarum is a precious traditional Chinese medicine with high nutritional and medicinal value, and has various biological activities such as anti-aging, nerve protection, anti-inflammation, promotion of metabolism, control of blood sugar, immune regulation and anti-tumor. Qinghai and Ningxia are the main producing areas of wolfberry in my country. Lycium barbarum has tenacious vitality, strong adaptability to the growth environment, strong salt and alkali tolerance, and can adapt to the harsh living environment in the western region, making it an important economy in the northwest region of my country. crops, the prospect of industrial development is very broad. [0003] Lycium barbarum root rot caused by Fusarium...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G13/00A01G17/00A01N63/22A01P3/00A01P21/00C12R1/07
Inventor 黄大野何嘉曹春霞张蓉杨丹郑娇莉王蓓蓓李飞
Owner HUBEI BIOPESTICIDE ENG RES CENT
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