Fluorescent probe for respectively and simultaneously detecting hydrogen sulfide and carbon monoxide as well as preparation method and application of fluorescent probe
A fluorescent probe and carbon monoxide technology, applied in the field of fluorescent probe detection, can solve the problems of interference between two probes, probe positioning, metabolism and photobleaching rates are different, affecting detection accuracy, etc. Good performance and high sensitivity
Active Publication Date: 2022-04-26
XUZHOU MEDICAL UNIV
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Problems solved by technology
In order to detect multiple analytes, one method is to use multiple fluorescent probes in one system, but this method has many defects: (1) there is interference between the two probes; The localization, metabolism and photobleaching rate are different, which will seriously affect the accuracy of the detection, so it is not suitable for quantitative analysis
Currently, H can be identified separately and simultaneously
2
Fluorescent probes for S and CO have not been reported
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[0077] 1. Solution configuration
[0078] Preparation of probe solution: FP-HSCO (7.72 mg, 0.01 mmol) was dissolved in acetonitrile (10 mL) to prepare a 1 mM probe solution.
[0079] Na 2 Preparation of S stock solution (as H 2 Source of S): Nitrogen gas was passed through 10 mL of deionized water for 15 min. Under nitrogen, the Na 2 S·9H 2O (24mg, 0.1mmol) was dissolved in the solution to make 10mM Na 2 S stock solution, Na 2 The S stock solution was diluted to 1.0mM-100μM solution for later use.
[0080] Na 2 S 2 Preparation of stock solution (as H 2 S 2 source): Nitrogen was passed through 10 mL of deionized water for 15 min. Under nitrogen, the Na 2 S 2 (11.01mg, 0.1mmol) was dissolved in the solution to make a concentration of 10mM Na 2 S 2 stock solution, Na 2 S 2 The stock solution was diluted to a solution of 1.0mM-100μM for use.
[0081] Na 2 S 4 Preparation of stock solution: Nitrogen gas was passed through 10 mL of deionized water for 15 min. Un...
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The invention relates to a fluorescent probe for respectively and simultaneously detecting hydrogen sulfide and carbon monoxide as well as a preparation method and application of the fluorescent probe, the structural formula of the fluorescent probe is shown in the specification, and the fluorescent probe has the advantages of low detection limit (the detection limits for detecting H2S and CO are respectively 9nM and 15nM), high sensitivity and good selectivity. The fluorescence intensity has a good linear relationship with the concentration of Na2S (0-40 [mu] M) and the concentration of CORM-3 (0-40 [mu] M), which indicates that the probe is suitable for quantitative detection of H2S and CO in a living body; and FP-HSCO is further applied to realize respective and simultaneous fluorescence imaging of H2S and CO in HT-22 cells.
Description
technical field [0001] The invention belongs to the technical field of fluorescent probe detection, and in particular relates to a fluorescent probe for separately and simultaneously detecting hydrogen sulfide and carbon monoxide, a preparation method and application thereof. Background technique [0002] Hydrogen sulfide (H 2 S) is the third endogenous gas signal molecule in organisms after nitric oxide and carbon monoxide (CO). Physiological levels of H 2 S is involved in a variety of biological functions, including nerve conduction, vasodilation, apoptosis, inflammation, ischemia / reperfusion injury, and insulin secretion. h 2 Abnormal S concentration is closely related to many diseases, such as Alzheimer's disease, Parkinson's disease and diabetes. CO is produced in biological systems through the decomposition of heme by heme oxygenase (HO-1), and is an important gas signal molecule in the human body. CO has various physiological functions such as anti-inflammation, ...
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IPC IPC(8): C07D491/107C09K11/06G01N21/64
CPCC07D491/107C09K11/06G01N21/6428C09K2211/1044C09K2211/1033C09K2211/1029C09K2211/1011Y02A50/20
Inventor 薛运生杨柳张玲陈佳胜腾杨欣郑友广
Owner XUZHOU MEDICAL UNIV