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Mixed probiotic system for fermenting modified corn flour and application

A technology of probiotics and corn flour, which is applied in mixed probiotic systems and application fields, can solve the problems of loss of nutrition and finished product quality, rough taste, poor processability of unfermented corn, etc., and achieve the goal of improving membrane permeability and strong starch degradation ability Effect

Pending Publication Date: 2022-05-06
哈尔滨拓百世环保涂料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The third technical problem to be solved by the present invention is that unfermented corn has poor processability, rough taste, and the fermented product has a unique fermented flavor, which is not suitable for the general public, and the nutrition and quality of the finished product are seriously lost after the fermented corn flour is cleaned.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of application of the mixed probiotic system for fermenting modified corn flour of the present embodiment, it is carried out according to the following steps:

[0034] Step 1. Bacterial activation and cultivation

[0035] Inoculate Bacillus natto, Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Lactobacillus plantarum into LB solid medium, LB solid medium, PDA solid medium and MRS solid medium respectively by streaking on the plate, at 32°C Cultivate for 24 hours at a temperature of -40°C;

[0036] Step 2. Bacteria expansion and cultivation

[0037] Bacillus natto, Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Lactobacillus plantarum after activation culture were inoculated into LB liquid medium, LB liquid medium, PDA liquid medium and MRS liquid medium respectively (the inoculum size was 1 %), cultivated for 24 hours at a temperature of 35°C-40°C and a rotating speed of 120r / min;

[0038] Step 3, corn puffing treatment

[0039] The puffed...

Embodiment 2

[0050] This embodiment is a synchronous test verification, and a comparative test group is set up: reduce the four bacterial strains in Example 1 by two strains, and retain Lactobacillus plantarum and Saccharomyces cerevisiae to investigate the synergistic effect of the four bacterial strains in Example 1. Wherein, the retained Lactobacillus plantarum: the ratio of Saccharomyces cerevisiae is 1:1 (the remaining experimental conditions are carried out according to the method of Example 1 for fermentation test).

[0051] The test result is: the product after the fermentation of embodiment 1 is mixed with embodiment 2 comparative test group and commercially available corn flour (purchased in the local market in Harbin, directly compared) to be mixed with 2.5% starch milk and carry out water holding capacity and gel strength measurement, carry out The water holding capacity of Example 1 is 3.15g / g, the water holding capacity of commercially available corn flour is 4.355g / g, and the...

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Abstract

The invention discloses a mixed probiotic system for fermenting modified corn flour and application, and relates to the field of fermentation. The defects that traditional corn flour is rough in taste and poor in processability are overcome. The preparation method comprises the steps of 1, strain preparation; 2, sample pretreatment; 3, performing semi-solid state fermentation; and 4, treatment after fermentation. Compared with the prior art, the method has the innovation points that bacteria and enzymes are synergistically combined, a biological technology is adopted, health hazards caused by chemical processing and food additives are eliminated, chemical bonds and loose structures in an endosperm protein network are damaged by lactic acid bacteria fermentation acid production, and saccharomycetes enzyme production modified protein is added; the enzyme produced by bacillus in the system fully permeates to modify starch in endosperm, and cellulase is combined with xylanase to act on corn seed coats to improve the membrane permeability of the corn seed coats and accelerate soaking. 2, the whole corn kernels are fermented, and all nutrients in corn seed coats and germs are reserved; and 3, the corn flour prepared by the method can be used for preparing various foods in the traditional staple food industry.

Description

technical field [0001] The invention is applied in the field of fermentation, and in particular relates to a mixed probiotic system for fermenting modified corn flour and its application. Background technique [0002] Corn is one of the important economic crops in my country, ranking first in the total output of the three major grains, with huge resources. Corn can not only provide calories as a staple food, but also a nutritious health food, rich in various vitamins and trace elements. Traditional corn flour does not contain gluten, so the resulting dough has poor viscoelasticity and is easy to split, making corn processing very dependent on wheat flour, and it is difficult to have more processing methods. The taste of traditional corn flour is rough, and in order to improve the taste, the germination and peeling are carried out during the production process, resulting in the loss of dietary fiber in the corn husk and nutrients such as lecithin in the germ. Therefore, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18A23L7/104A23L7/165A23L7/152A23L7/10C12R1/07C12R1/865C12R1/25
CPCC12N1/20C12N1/18A23L7/104A23L7/165A23L7/152A23L7/115A23V2002/00A23V2400/169A23V2250/762
Inventor 吴征王松史丽琦阎菲
Owner 哈尔滨拓百世环保涂料有限公司
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