Mixed probiotic system for fermenting modified corn flour and application
A technology of probiotics and corn flour, which is applied in mixed probiotic systems and application fields, can solve the problems of loss of nutrition and finished product quality, rough taste, poor processability of unfermented corn, etc., and achieve the goal of improving membrane permeability and strong starch degradation ability Effect
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Embodiment 1
[0033] A kind of application of the mixed probiotic system for fermenting modified corn flour of the present embodiment, it is carried out according to the following steps:
[0034] Step 1. Bacterial activation and cultivation
[0035] Inoculate Bacillus natto, Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Lactobacillus plantarum into LB solid medium, LB solid medium, PDA solid medium and MRS solid medium respectively by streaking on the plate, at 32°C Cultivate for 24 hours at a temperature of -40°C;
[0036] Step 2. Bacteria expansion and cultivation
[0037] Bacillus natto, Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Lactobacillus plantarum after activation culture were inoculated into LB liquid medium, LB liquid medium, PDA liquid medium and MRS liquid medium respectively (the inoculum size was 1 %), cultivated for 24 hours at a temperature of 35°C-40°C and a rotating speed of 120r / min;
[0038] Step 3, corn puffing treatment
[0039] The puffed...
Embodiment 2
[0050] This embodiment is a synchronous test verification, and a comparative test group is set up: reduce the four bacterial strains in Example 1 by two strains, and retain Lactobacillus plantarum and Saccharomyces cerevisiae to investigate the synergistic effect of the four bacterial strains in Example 1. Wherein, the retained Lactobacillus plantarum: the ratio of Saccharomyces cerevisiae is 1:1 (the remaining experimental conditions are carried out according to the method of Example 1 for fermentation test).
[0051] The test result is: the product after the fermentation of embodiment 1 is mixed with embodiment 2 comparative test group and commercially available corn flour (purchased in the local market in Harbin, directly compared) to be mixed with 2.5% starch milk and carry out water holding capacity and gel strength measurement, carry out The water holding capacity of Example 1 is 3.15g / g, the water holding capacity of commercially available corn flour is 4.355g / g, and the...
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