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Preparation method and microscopic examination method of chitosan embedded calcium alginate microcapsule

A technology of calcium alginate and microcapsules, which is applied in the field of microcapsules, can solve the problems that there is no simple way to observe microcapsules, calcium alginate microcapsules require high sample preparation, and take a long time to achieve low cost of microscope inspection equipment, The microscopic inspection process is fast and convenient, and the effect of low shape requirements

Pending Publication Date: 2022-05-10
山东泰山生力源集团股份有限公司
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Problems solved by technology

[0002] At present, there are many methods for embedding calcium alginate microcapsules in chitosan, such as using sodium alginate and calcium carbonate to make them, or directly dropping the mixed solution of chitosan and calcium chloride into the sodium alginate solution to form microcapsules. Capsules, etc. However, the chitosan-embedded calcium alginate microcapsules prepared by the existing methods need to be freeze-dried and then observed with a scanning electron microscope. There is no simple way to observe the state of the microcapsules after secondary embedding in chitosan
Therefore, the existing chitosan-embedded calcium alginate microcapsules have high requirements for sample preparation, the observation method takes a long time, and the equipment cost is high

Method used

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  • Preparation method and microscopic examination method of chitosan embedded calcium alginate microcapsule
  • Preparation method and microscopic examination method of chitosan embedded calcium alginate microcapsule
  • Preparation method and microscopic examination method of chitosan embedded calcium alginate microcapsule

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preparation example Construction

[0048] like figure 1 As shown, the preparation method of chitosan-embedded calcium alginate microcapsules in this embodiment includes the following steps:

[0049] S10. Mix the active microorganisms in the preset ratio with the biological dye to obtain the dyed active microorganism liquid.

[0050] In a preferred embodiment, the active microorganisms are concentrated edible fungi, preferably concentrated lactic acid bacteria, and the concentration of lactic acid bacteria is 2.0-4.0×10 11 CFU / mL.

[0051]The biological stain is a Gram stain, preferably a safranine stain and a crystal violet stain. Safranin is a basic dye that is soluble in water and can bind to nucleic acid in the nucleus and dye the nucleus red; crystal violet is also a basic dye that is soluble in water and can bind to nucleic acid in the nucleus and dye the nucleus. Dyed blue. In this example, safranin / crystal violet staining solution was used to dye the lactic acid bacteria sclerotia into red or blue. ...

Embodiment 1

[0091] First, 87.5ml concentration is 2.4 × 10 11 The lactic acid bacteria solution of CFU / ml was mixed with 87.5ml 25% safranine dye solution for 20min to obtain the lactic acid bacteria solution after staining. The concentration of the lactic acid bacteria solution after staining was 1.2×10 11 CFU / ml

[0092] Then, the dyed lactic acid bacteria solution, 4% sodium alginate solution, 5% sodium carbonate solution, and 10% calcium chloride solution were stirred and mixed evenly according to the volume ratio of 175ml: 225ml: 36ml: 19ml, and then allowed to stand at 4°C for 1 hour. After degassing, a staining suspension was obtained. Then, according to the water-oil volume ratio of 30:100, the above dyeing suspension was added to soybean oil (containing 1.0% span80), and stirred for 30min at a rotating speed of 300r / min to form a water-in-oil (w / o) dyeing emulsion. In the obtained dyed emulsion, soybean oil containing 25% glacial acetic acid was added dropwise, and HAC and CaCO...

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Abstract

The invention discloses a preparation method and a microscopic examination method of chitosan-embedded calcium alginate microcapsules. The preparation method comprises the following steps: mixing active microorganisms and a biological coloring agent according to a preset ratio to obtain a dyed active microorganism liquid; mixing soluble alginate, soluble carbonate, soluble calcium salt, water and the dyed lactic acid bacteria liquid according to a preset ratio, and performing primary embedding to obtain dyed calcium alginate microcapsule particles; adding the obtained dyed calcium alginate microcapsule particles into a chitosan solution to obtain a chitosan-embedded calcium alginate microcapsule subjected to secondary embedding; compared with the current situation that the chitosan-embedded calcium alginate microcapsule prepared by the existing method has higher requirements on the sample form and equipment, the chitosan-embedded calcium alginate microcapsule prepared by the preparation method has a certain color development effect, can be clearly observed under an optical microscope, has low requirements on the microscopic examination sample form, is low in microscopic examination equipment cost, and is suitable for industrial production. The microscopic examination process is fast and convenient, and the microscopic examination result is accurate.

Description

technical field [0001] The invention relates to the technical field of microcapsules, in particular to a preparation method and a microscope inspection method of chitosan-embedded calcium alginate microcapsules. Background technique [0002] At present, there are many methods for embedding calcium alginate microcapsules with chitosan, such as using sodium alginate and calcium carbonate to make, or directly dropping the mixed solution of chitosan and calcium chloride into sodium alginate solution to react to form microcapsules. Capsules, etc. However, the chitosan-embedded calcium alginate microcapsules prepared by the existing method need to be observed by scanning electron microscope after freeze-drying. Therefore, the existing chitosan-embedded calcium alginate microcapsules require high sample preparation, the observation method takes a long time, and the equipment cost is high. [0003] Therefore, it is necessary to find a preparation method of chitosan-embedded calcium...

Claims

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Application Information

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IPC IPC(8): B01J13/02G01N21/84G01N1/38G01N1/30C12N11/10C12N11/04
CPCB01J13/02G01N21/84G01N1/38G01N1/30C12N11/10C12N11/04
Inventor 宋洪宁李军训马艳芳马正阳徐启民
Owner 山东泰山生力源集团股份有限公司
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