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Electrochemical enzyme biosensor for detecting organophosphorus pesticide as well as preparation method and application of electrochemical enzyme biosensor

A biosensor and organophosphorus pesticide technology, applied in the field of electrochemical analysis, can solve the problems of complex pretreatment, long detection cycle, difficult sample screening and detection, etc., and achieve the effect of good selectivity, simple operation and fast detection

Active Publication Date: 2022-05-13
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Common pesticide detection techniques mainly include gas chromatography, high performance liquid chromatography, chromatography-mass spectrometry, etc. Most of these methods rely on large-scale laboratory instruments, and have the disadvantages of complex pretreatment and long detection cycle, so it is difficult to realize them in a short time Screening and testing of a large number of samples

Method used

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  • Electrochemical enzyme biosensor for detecting organophosphorus pesticide as well as preparation method and application of electrochemical enzyme biosensor
  • Electrochemical enzyme biosensor for detecting organophosphorus pesticide as well as preparation method and application of electrochemical enzyme biosensor
  • Electrochemical enzyme biosensor for detecting organophosphorus pesticide as well as preparation method and application of electrochemical enzyme biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of an electrochemical enzyme biosensor for detecting organophosphorus pesticides:

[0032] (1) Preparation of covalent organic framework / multi-walled carbon nanotube composites

[0033] Disperse the multi-walled carbon nanotubes in the mixed solution of 1-butanol and 1,2-dichlorobenzene to obtain dispersion A, wherein, every 6mL of the mixed solution of 1-butanol and 1,2-dichlorobenzene Correspondingly, 3 mg of multi-walled carbon nanotubes were added, and the volume ratio of 1-butanol to 1,2-dichlorobenzene in the mixed solution was 1:1.

[0034] Disperse 2,5-dihydroxyterephthalaldehyde and 1,3,5-tris(4-aminophenyl)benzene in acetic acid to obtain dispersion B and dispersion C, in which 2,5-dihydroxyterephthalaldehyde The molar ratio of phthalaldehyde to 1,3,5-tris(4-aminophenyl)benzene is 3:2, and 0.2 mmol of 2,5-dihydroxyterephthalaldehyde is added to every 0.6 mL of acetic acid.

[0035] Ultrasonically mixes the uniform dispersion liquid AB an...

Embodiment 2

[0044] A preparation method of an electrochemical enzyme biosensor for detecting organophosphorus pesticides:

[0045] (1) Preparation of covalent organic framework / multi-walled carbon nanotube composites

[0046] Disperse the multi-walled carbon nanotubes in the mixed solution of 1-butanol and 1,2-dichlorobenzene to obtain dispersion A, wherein, every 6mL of the mixed solution of 1-butanol and 1,2-dichlorobenzene Correspondingly, 24 mg of multi-walled carbon nanotubes were added, and the volume ratio of 1-butanol to 1,2-dichlorobenzene in the mixed solution was 1:1.

[0047] Disperse 2,5-dihydroxyterephthalaldehyde and 1,3,5-tris(4-aminophenyl)benzene in acetic acid to obtain dispersion B and dispersion C, in which 2,5-dihydroxyterephthalaldehyde The molar ratio of phthalaldehyde to 1,3,5-tris(4-aminophenyl)benzene is 3:2, and 0.3 mmol of 2,5-dihydroxyterephthalaldehyde is added to every 0.6 mL of acetic acid.

[0048] Ultrasonically mixes the uniform dispersion liquid AB a...

Embodiment 3

[0057] A preparation method of an electrochemical enzyme biosensor for detecting organophosphorus pesticides:

[0058] (1) Preparation of covalent organic framework / multi-walled carbon nanotube composites

[0059] Disperse the multi-walled carbon nanotubes in the mixed solution of 1-butanol and 1,2-dichlorobenzene to obtain dispersion A, wherein, every 6mL of the mixed solution of 1-butanol and 1,2-dichlorobenzene Correspondingly, 12 mg of multi-walled carbon nanotubes were added, and the volume ratio of 1-butanol to 1,2-dichlorobenzene in the mixed solution was 1:1.

[0060] Disperse 2,5-dihydroxyterephthalaldehyde and 1,3,5-tris(4-aminophenyl)benzene in acetic acid to obtain dispersion B and dispersion C, in which 2,5-dihydroxyterephthalaldehyde The molar ratio of phthalaldehyde to 1,3,5-tris(4-aminophenyl)benzene is 3:2, and 0.24 mmol of 2,5-dihydroxyterephthalaldehyde is added to every 0.6 mL of acetic acid.

[0061] Ultrasonically mixes the uniform dispersion liquid AB ...

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Abstract

The invention provides an electrochemical enzyme biosensor for detecting organophosphorus pesticides, a preparation method and application, and belongs to the field of electrochemical analysis. The electrochemical enzyme biosensor is prepared by sequentially modifying a covalent organic framework / multi-walled carbon nanotube composite material and acetylcholin esterase on the surface of a glassy carbon electrode, the covalent organic framework / multi-walled carbon nanotube composite material takes a multi-walled carbon nanotube as a carrier, and a covalent organic framework material is formed on the surface of the carrier. A three-electrode system is adopted, namely a reference electrode is a silver / silver chloride electrode, a counter electrode is a platinum wire electrode, a working electrode is formed by sequentially modifying a covalent organic framework / multi-walled carbon nanotube composite material and acetylcholin esterase on the surface of a glassy carbon electrode, and the malathion is detected by adopting a differential pulse voltammetry. The electrochemical enzyme biosensor constructed by the invention has the advantages of high sensitivity, simple preparation method, high selectivity and the like, and has good application prospect and value in the field of organophosphorus pesticide residue detection.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis, and relates to an electrochemical enzyme biosensor for detecting organophosphorus pesticides, a preparation method and an application. Background technique [0002] With the improvement of people's living standards, in order to improve agricultural productivity, pesticides have been widely used in agricultural production activities. Pesticides can be classified into organophosphorus pesticides, organochlorine pesticides, carbamate pesticides and pyrethroid pesticides. However, in the actual application process, only about 0.1% of pesticides can play their role, and more than 90% of pesticides will enter the environment through processes such as biodegradation, hydrolysis, photolysis, evaporation, and surface runoff, causing negative impacts on the environment At the same time, trace amounts of pesticides remaining in water, soil, and agricultural products can enter the human body through ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 王雪杨爽单佳佳白旭婷
Owner DALIAN UNIV OF TECH
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