Grease-rich fiber fusiformis and culture application thereof
A spindle and fiber technology, applied in the field of spindle fiber algae and its cultivation application, can solve the problems of high energy consumption of microalgae, stop growing, slow growth of microalgae, etc.
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Embodiment 1
[0023] Example 1 Separation and domestication screening to obtain Spinocystis HC-DY4
[0024] (1) Obtaining the starting algae strain: In October 2016, 150mL of water samples were retrieved from the Hunchun River in Hunchun City, Jilin Province, filtered with gauze to remove large impurities, and 50ml of the filtered water samples were inoculated into 200mL of BG11 The enrichment culture was carried out in the culture medium, the light intensity of the culture was 5000Lux, the temperature was 15°C, the light cycle was 24h, and the light-dark time ratio was 14:10. After about half a month of culture, the culture medium turned green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000 Lux, and the temperature is 15°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shake...
Embodiment 2
[0029] Example 2 Identification of algae strains
[0030] The DNA of HC-DY4 algae cells was extracted by CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Ankistrodesmus fusiformis It has the greatest similarity, its BLASTn value is 2848, and its Max index value is 99.68%. It can be determined that HC-DY4 is a spindle cell algae ( Ankistrodesmus fusiformis ).
Embodiment 3
[0031] The application of embodiment 3 spindle cell algae HC-DY4
[0032] Inoculate the algae liquid of the spindle cell algae in the logarithmic growth phase in the BG11 medium for cultivation. The formula of the BG11 medium is shown in Table 1 and Table 2. The cultivation is carried out in a photobioreactor. The OD of the culture solution after inoculation is 690 is 0.23. CO was introduced from the bottom of the reactor 2Flue gas with a content of 40v%, of which the NO content is 0.07%. . During the cultivation process, the light intensity was 8000Lux, the cultivation temperature was 20°C, the pH value was controlled at 7-8, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 8 days. After the cultivation was completed, the algae liquid was collected by centrifugation. After vacuum freeze-drying at -60°C to constant weight, the dry weight of the algae powder was measured, the biomass production was calculated, and the total lipid con...
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