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Green alga strain and application thereof in rare earth ammonia nitrogen wastewater treatment

A technology of ammonia nitrogen wastewater and bacterial strains, applied in biological water/sewage treatment, water/sludge/sewage treatment, water pollutants, etc.

Active Publication Date: 2022-06-10
福州文泽生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the purpose of the present invention is to separate and screen a kind of strong tolerance from high-concentration rare earth ammonia nitrogen sewage, and can use cheap and convenient source Greenhouse gas CO2 is the carbon source, and microalgae, which uses clean energy and sunlight as the main energy source, is expected to establish a stable and efficient rare earth sewage denitrification process through this microorganism, reduce the power consumption and energy consumption of operation, and thus solve the problem of Ammonia nitrogen pollution problem that plagues the surrounding environment of the rare earth industry

Method used

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  • Green alga strain and application thereof in rare earth ammonia nitrogen wastewater treatment

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Embodiment 1

[0008] Example 1 Screening, isolation and identification of bacterial strains

[0009] 2) Microalgae without ammonia nitrogen tolerance are inhibited by high-concentration ammonia-nitrogen wastewater and cannot grow. Microalgae with tolerance can also use ammonia nitrogen in high-concentration ammonia-nitrogen wastewater to synthesize their own cell matrix and grow. After enrichment, microalgae with high ammonia nitrogen tolerance were screened out.

[0010] 3) Screening and separation of microalgae

[0011] 4) Purification of microalgae. Add penicillin and gentamicin to the green culture in the test tube to make the final concentrations of the two antibiotics in the test tube 20-100 μg / mL and 15-50 μg / mL, and continue to cultivate for one day. Dilute the green culture in the test tube with sterile water to an appropriate multiple. After the gradient dilution, take 100 μL and spread it on a solid plate containing solid phosphorus-containing rare earth sewage, and cultivate i...

Embodiment 2

[0015] Application example 2

2) The isolated and purified microalgae Chlamydomonas The sp. YC strain was placed in phosphorus-containing rare-earth ammonia-nitrogen sewage liquid, and cultured at 25°C, 150 r / min, 2500 lux constant temperature and light for 5 days to prepare microalgae YC seed liquid. Take 100 mL of microalgae YC seed solution, centrifuge and transfer the sediment into 1 L of raw rare earth sewage (ammonia nitrogen content is 2000 mg / L), and culture at 25 °C and 700 mL / min aeration. The 2000 mg / L ammonia nitrogen in the rare earth sewage was reduced to 350 mg / L within 50 days, and the ammonia nitrogen consumption rate reached 82.5%.

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Abstract

The invention discloses a green alga strain and application thereof in rare earth ammonia nitrogen wastewater treatment, the green alga strain is classified and named as Chlamydomonas YC, the chemical name is Chlamydomonas sp.YC, the green alga strain is preserved in China Center for Type Culture Collection in Wuhan on May 13, 2021, and the preservation number is CCTCC NO: M 2021529. The green algae strain can be used for treating high-concentration ammonia nitrogen wastewater.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a green algae strain and its application in the treatment of rare earth ammonia nitrogen wastewater. Background technique [0002] The current rare earth mining technology mainly adopts the in-situ leaching process. In this process, liquid injection wells are arranged on the surface of the mountain, and ammonium sulfate solution configured to a certain concentration is injected into the ore body as a leaching agent to replace the rare earth in the rare earth ore, and the leachate is collected. Enrichment of carbonate rare earth products by ammonium bicarbonate precipitation. In-situ leaching does not require stripping of topsoil and excavation of the mountain, which causes less damage to the vegetation of the mountain and does not generate a large amount of tailings. Because a large amount of ammonium sulfate was invested in the leaching, the soil and tail wat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89C02F101/16
CPCC02F3/322C02F2101/16Y02W10/10
Inventor 王明兹周有彩肖雪花何勇锦陈必链
Owner 福州文泽生物科技有限公司
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