Biological FeS nanoparticle reinforced microbial agent, preparation method thereof and method for applying microbial agent to denitrification

A microbial agent and nanoparticle technology, which is applied in the field of nitrogen-containing wastewater treatment, can solve the problems of low efficiency and achieve the effects of simple preparation, increased electron transfer capacity, and wide source of materials

Pending Publication Date: 2022-07-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacteria often need to communicate through interspecies electron transfer to complete their own metabolism, but the efficiency of electron transfer between bacteria through direct contact is very low. Biological FeS nanoparticles can be used as nanowire analogs to increase the electron transfer ability between cells , to promote the mutual symbiosis of electrons between different bacteria, and enhance the ability of cell metabolism and degradation of pollutants

Method used

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  • Biological FeS nanoparticle reinforced microbial agent, preparation method thereof and method for applying microbial agent to denitrification
  • Biological FeS nanoparticle reinforced microbial agent, preparation method thereof and method for applying microbial agent to denitrification
  • Biological FeS nanoparticle reinforced microbial agent, preparation method thereof and method for applying microbial agent to denitrification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Removal of NH-containing microbial agents 4 + -N and NO 3 - -N performance of wastewater

[0040] Prepare the microbial inoculum as follows:

[0041] Pseudomonas stutzeri (Pseudomonas stutzeri), Paracoccus denitrificans (Paracoccus denitrificans), Bacillus megaterium (Bacillusmegaterium), Achromobacter denitrificans (Achromobacter denitrificans), Bacillus subtilis ( Bacillus subtilis), Lactobacillus acetotoleran (Lactobacillus acetotoleran) and Citrobacter freundii (Citrobacterfreundii) were separately cultured in the medium to obtain Pseudomonas stutzeri culture, Paracoccus denitrificans culture, Bacillus megaterium culture, colorless Denitrifying bacteria culture, Bacillus subtilis culture, lactic acid bacteria culture and Citrobacter freundii culture, respectively, centrifuge the cultures, discard the supernatant, resuspend to obtain a suspension of Pseudomonas stutzeri, denitrify Paracoccus suspension, Bacillus megaterium suspension, colorless denitr...

Embodiment 2

[0047] Example 2: Removal of NH-containing microbial inoculants loaded with biological FeS nanoparticles 4 + -N and NO 3 - -N performance of wastewater

[0048] Follow the steps below to prepare the microbial inoculum loaded with biological FeS nanoparticles:

[0049] (1) Preparation of biological FeS nanoparticles:

[0050] Pick two rings of purchased Citrobacter freundii (Citrobacter freundii) and inoculate it into LB liquid medium (10g / L peptone, 10g / L NaCl, 5g / L yeast powder, the rest are water), at 150rpm, 30 ℃ Cultivate for 2 days, transfer Citrobacter freundii to Citrobacter freundii medium at an inoculum of 1 wt%, and cultivate for 4 days at 150 rpm and 30°C under anaerobic conditions to obtain the in situ synthesis of Citrobacter freundii. of biological FeS nanoparticles.

[0051] (2) Preparation of microbial inoculum:

[0052] The purchased Pseudomonas stutzeri, Paracoccus denitrificans, Bacillus megaterium, Achromobacterdenitrificans, Bacillus subtilis and La...

Embodiment 3

[0060] Example 3: Biological FeS Nanoparticles Enhanced Microbial Agent Removal of NH-containing 4 + -N and NO 3 - -N the application of actual fermentation type pharmaceutical wastewater.

[0061] Follow the steps below to prepare the microbial inoculum loaded with biological FeS nanoparticles:

[0062] (1) Preparation of biological FeS nanoparticles:

[0063] Pick two rings of purchased Citrobacter freundii (Citrobacter freundii) and inoculate it into LB liquid medium (10g / L peptone, 10g / L NaCl, 5g / L yeast powder, the rest are water), at 150rpm, 30 ℃ Cultivate for 2 days, transfer Citrobacter freundii to Citrobacter freundii medium at an inoculum of 1 wt%, and cultivate for 4 days at 150 rpm and 30°C under anaerobic conditions to obtain the in situ synthesis of Citrobacter freundii. of biological FeS nanoparticles.

[0064] (2) Preparation of microbial inoculum:

[0065] The purchased Pseudomonas stutzeri, Paracoccus denitrificans, Bacillus megaterium, Achromobacterde...

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Abstract

The invention discloses a biological FeS nanoparticle reinforced microbial agent, a preparation method thereof and a method for applying the microbial agent to denitrification. The preparation method comprises the preparation of the biological FeS nano particles and the microbial agent and the application of the biological FeS nano particles and the microbial agent. The preparation of the biological FeS nano particles is characterized in that the biological FeS nano particles are synthesized in situ by utilizing citrobacter freundii under an anaerobic condition. The microbial agent comprises pseudomonas stutzeri, paracoccus denitrificans, bacillus megatherium, achromobacter denitrificans, bacillus subtilis and lactic acid bacteria, and the microbial agent is cultured under the aerobic condition. And adding the FeS nanoparticles into the microbial agent to obtain the microbial agent loaded with the biological FeS nanoparticles. After the microbial agent loaded with the biological FeS nanoparticles is activated, nitrate nitrogen and ammonia nitrogen in a water body are effectively removed under the heterotrophic aerobic condition, the microbial agent is simple to prepare, the biological denitrification effect can be effectively enhanced, and the microbial agent has great application potential in the aspect of organic water body denitrification.

Description

technical field [0001] The invention relates to the field of nitrogen-containing wastewater treatment, in particular to a biological FeS nanoparticle-enhanced microbial inoculant, a preparation method thereof, and a method for application to denitrification. Background technique [0002] Excessive nitrogen in the water body will lead to eutrophication of the water body, resulting in the deterioration of water quality, endangering the life of animals and plants in the water, and even threatening human health. The effective removal of nitrogen pollution in water bodies is a global concern. Biological nitrogen removal has the characteristics of high efficiency, economy and greenness, and has been widely studied and put into use. [0003] In order to remove ammonia nitrogen and nitrate nitrogen in water, aerobic nitrification and anaerobic denitrification are generally used in traditional processes. However, these two traditional processes consume a lot of energy and the microo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00B82Y30/00C02F3/34C12R1/38C12R1/025C12R1/01C12R1/11C12R1/125C02F101/38
CPCC12N1/20C12P3/00B82Y30/00C02F3/34C02F2101/38
Inventor 陈元彩王婧豪林文敏
Owner SOUTH CHINA UNIV OF TECH
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