Lactobacillus paracasei and application thereof in prevention of streptococcus infection of infants

A technology of Lactobacillus and paracheese, which is applied to medical preparations containing active ingredients, bacteria, antibacterial drugs, etc., can solve problems such as no necrotizing enterocolitis or septicemia prevention effect.

Pending Publication Date: 2022-07-05
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nonetheless, Garland SM et al. found equivocal effects of probiotics on the incidence or severity of sepsis in preterm infants. In a trial of 1310 pre

Method used

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  • Lactobacillus paracasei and application thereof in prevention of streptococcus infection of infants
  • Lactobacillus paracasei and application thereof in prevention of streptococcus infection of infants
  • Lactobacillus paracasei and application thereof in prevention of streptococcus infection of infants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening of Lactobacillus paracasei VHProbi F11

[0029] 1. Bacteria isolation

[0030] Prepare MRS (Man Rogosa Sharpe) broth: pure water 1L, peptone 10g, beef extract 10g, yeast extract 5.0g, sodium acetate 5g, glucose 5g, potassium dihydrogen phosphate 2g, Tween 80 1.0mL, citric acid Diamine 2.0g, calcium carbonate 20g, magnesium sulfate heptahydrate 0.58g, manganese sulfate heptahydrate 0.25g, adjusted pH 6.2-6.5.

[0031] Configure MRS agar medium: add 15g agar to 1LMRS broth.

[0032] According to the 2019 version of the "Human Genetic Resource Bank Ethics Code", after signing the project commitment letter and informed consent with the sample provider, in accordance with the standard operating specifications of the biobank, take 1g of fresh fecal samples from healthy infants who have not consumed probiotic preparations within half a year. , diluted with sterile physiological saline, put it into a sterile sample bag, beat and mixed with a homogenizer; t...

Embodiment 2

[0042] Example 2 Identification of F11 strain

[0043] 1. Identification of colony morphology

[0044] The F11 strain was inoculated on the MRS agar medium, and after anaerobic culture at 37°C for 24 hours, the single colony of F11 was milky white, the colony diameter was about 1-2mm, the surface was smooth, the edges were round and convex, and the strain under the microscope was bacilli. .

[0045] 2. Identification of physiological and biochemical characteristics

[0046] The preparation of the inoculum in this example is as follows: under aseptic conditions, take an appropriate amount of fresh F11 bacterial solution, centrifuge at 5000 rpm / min for 5 min, wash twice with PBS buffer, and then reconstitute the bacteria with the same volume of PBS buffer and dilute 50 times as the inoculum.

[0047] 2.1. Salinity tolerance test

[0048] Under sterile conditions, add 190 μL of BSM liquid medium with a salt concentration of 1%, 2%, 3%, 4%, 5%, 6%, 7%, and 8% to a 96-well plat...

Embodiment 3

[0104] Example 3 Tolerance test of Lactobacillus paracasei VHProbi F11 to artificial gastric juice

[0105] 1. Preparation of artificial gastric juice

[0106] Weigh 5 g of peptone, 2.5 g of yeast extract, 1 g of glucose and 2 g of NaCl, add 1000 mL of distilled water, adjust pH to 3.0 with dilute hydrochloric acid, and then sterilize at 115 °C for 20 min. Then add 3.2 g of porcine mucosal pepsin before use, shake well to dissolve, and place in a warm water bath for 1 hour in a 37°C water bath shaker to simulate human body temperature.

[0107] 2. Test method

[0108] Take 2 mL of fresh bacterial liquid, centrifuge at 5000 rpm / min for 5 min to collect the bacterial cells, wash the bacterial cells three times with normal saline, and resuspend with 2 mL of normal saline as the inoculum. Take 1 mL of inoculum, add it to 24 mL of artificial gastric juice, place it on a water bath shaker (200 rpm / min) at 37 °C for 3 h, and sample 1 mL to detect the amount of viable bacteria.

[...

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Abstract

The invention belongs to the technical field of screening and application of probiotics, and particularly relates to a novel lactobacillus paracasei strain and an application of the lactobacillus paracasei strain. The provided lactobacillus paracasei is separated from fresh excrement of healthy infants, and is preserved in the China Center for Type Culture Collection on May 24, 2021, and the preservation number of the lactobacillus paracasei is CCTCC (China Center for Type Culture Collection) NO: M2021590. The strain has a very strong antibacterial effect on beta-hemolytic streptococcus, can improve the immunity of a host, relieve inflammatory response and effectively prevent and treat infection caused by streptococcus, and is particularly suitable for preventing streptococcus infection of infants.

Description

technical field [0001] The invention belongs to the technical field of screening and application of probiotics, in particular to a strain of Lactobacillus paracasei and its application in preventing Streptococcus infection of infants and young children. Background technique [0002] Group B Streptococcus (GBS) is a β-hemolytic Gram-positive streptococcus, and its pathogenicity mostly occurs in immunocompromised patients, the elderly, pregnant women and newborns. According to statistics, about 20% of healthy women carry GBS, which can lead to a series of adverse pregnancy outcomes such as premature rupture of membranes, late miscarriage, premature birth, and intrauterine dysplasia, as well as neonatal sepsis, pneumonia, meningitis, and even neonatal septicemia. Therefore, GBS occupies an important position in perinatal infectious diseases. In addition, due to the low immune function of infants, it is easy to infect Streptococcus in the environment through the skin or mucous ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61K8/99A61P31/04A61P39/06A61Q19/00A61Q17/00C12R1/225
CPCA23L33/135A61K35/747A61K8/99A61P31/04A61P39/06A61Q19/00A61Q17/00A23V2002/00A23V2400/165A23V2200/30A23V2200/3262Y02A50/30
Inventor 倪海平段治刘金王倩郭超群崔洪昌张景燕吴松洁李凯玲
Owner QINGDAO VLAND BIOTECH GRP
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