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HPPI-TOFMS-based early-stage lung cancer diagnosis expiratory gas marker screening and application thereof

A technology for lung cancer and auxiliary diagnosis, which is applied in the field of medical diagnosis and can solve the problems of increasing the complexity of data analysis, cumbersome preprocessing steps, time-consuming detection process, limitations, etc.

Pending Publication Date: 2022-07-08
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although GC-MS technology is mature, cumbersome pretreatment steps and time-consuming detection process limit its application
Other direct mass spectrometric detection methods, such as secondary electrospray ionization, selective ion flow tubes, and proton transfer reactions, have also been used for rapid detection of exhaled breath; however, the large amount of water vapor in exhaled breath complicates the ionization process and increases the Complexity of Data Analysis

Method used

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  • HPPI-TOFMS-based early-stage lung cancer diagnosis expiratory gas marker screening and application thereof
  • HPPI-TOFMS-based early-stage lung cancer diagnosis expiratory gas marker screening and application thereof
  • HPPI-TOFMS-based early-stage lung cancer diagnosis expiratory gas marker screening and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1. Screening of biomarkers (discovery studies)

[0095] The flow chart of discovery research is as follows figure 1 shown.

[0096] 1. Subject screening and subject information

[0097] The discovery study was conducted at Peking University People's Hospital from September to December 2020. Breath samples were collected at three time points, the morning of the day of surgery (preoperative), 3 days postoperatively, and 4 weeks postoperatively. This study was approved by the Ethics Committee of Peking University People's Hospital (2019PHB095-01). All patients were informed about the study protocol and obtained written consent prior to study entry. The study was conducted in accordance with the Standards for Reporting Diagnostic Accuracy (STARD) reporting guidelines (Bossuyt PM, Reitsma JB, Bruns DE, Gatsonis CA, Glasziou PP, Irwig L, et al. STARD 2015: an updated list of essentialitems for reporting diagnostic accuracy studies. BMJ.2015;351:h5527.DOI:10.1136 / ...

Embodiment 2

[0118] Example 2. Confirmation and Evaluation of Biomarkers (Confirmation Study)

[0119] The flow chart of the confirmatory study is as follows Figure 4 shown.

[0120] 1. Subject screening and subject information

[0121] The above-mentioned lung cancer exhalation markers were verified in the First Affiliated Hospital of Zhengzhou University.

[0122] Inclusion criteria for lung cancer patients in the confirmatory study were the same as in the discovery study; breath samples were collected on the day of surgery or biopsy (preoperatively). A healthy person in a confirmatory study was defined as a person who underwent an LDCT examination with no positive result; a breath sample was collected on the day of the examination. All participants were instructed to fast for at least 8 hours and not to consume spicy food, alcohol or coffee the night before breath collection.

[0123] The confirmatory study included 157 lung cancer patients and 368 healthy individuals. Subject cha...

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Abstract

The invention discloses HPPI-TOFMS (Hydrophile Peroxidase-Time of Flight Mass Spectrometry) expiratory gas marker screening for early lung cancer diagnosis and application thereof. The invention provides an application of 16 volatile organic compounds as lung cancer expiration biomarkers in development of products for diagnosis or auxiliary diagnosis of lung cancer. The 16 volatile organic compounds are acetaldehyde, 2-hydroxyacetaldehyde, isoprene, valeraldehyde, butyric acid, methylbenzene, 2, 5-dimethylfuran, cyclohexanone, hexanal, heptanal, acetophenone, propyl cyclohexane, octanal, nonanal, capraldehyde and 2, 2-dimethyl decane. The inventor of the invention determines 16 exhalation biomarkers of lung cancer by using HPPI-TOFMS for perioperative period exhalation omics detection, the combination of the 16 exhalation biomarkers can distinguish lung cancer patients from healthy people, and the 16 exhalation biomarkers can be used for clinical practice to optimize lung cancer screening schemes.

Description

technical field [0001] The invention belongs to the field of medical diagnosis, relates to the screening of exhaled breath markers for early lung cancer diagnosis based on HPPI-TOFMS and its application, and specifically provides the development of 16 volatile organic compounds VOCs as lung cancer exhaled biomarkers and their application in the diagnosis of lung cancer. application. Background technique [0002] Lung cancer is the leading cause of cancer-related death worldwide. Early diagnosis and treatment are critical to improving lung cancer survival. However, the early detection and diagnosis of lung cancer remains challenging due to the lack of early clinical manifestations and specific biomarkers. Annual low-dose computed tomography (LDCT) screening can significantly reduce lung cancer-specific mortality. However, the application of LDCT screening is challenging in several ways. High cost, radiation exposure, and high false-positive rates hinder the use of LDCT fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N33/497G16C20/20G16C20/70G16H50/20G16H10/20G16H10/40
CPCG01N27/62G01N33/497G16C20/20G16C20/70G16H50/20G16H10/20G16H10/40
Inventor 邱满堂王培宇孟漱石王少东李庆运王东鉴
Owner PEOPLES HOSPITAL PEKING UNIV
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