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Wall-mounted ultrasonic enhanced fermentation device suitable for large fermentation tank and fermentation method of wall-mounted ultrasonic enhanced fermentation device

A fermentation device, ultrasonic technology, applied in the field of wall-mounted ultrasonic intensified fermentation device, can solve the problems of uneven ultrasonic effect, sound energy attenuation, large diameter and height, etc., to speed up the cleaning process, facilitate disassembly, and low energy consumption Effect

Pending Publication Date: 2022-07-12
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a wall-mounted ultrasonic intensified fermentation device suitable for large-scale fermentation tanks. By hanging multiple groups of ultrasonic transducers on the inner wall of the fermentation tank in a balanced manner, they can directly act on the fermentation liquid and overcome the problems caused by the thickness of the large-scale fermentation tank. The sound energy attenuation is serious, and the ultrasonic effect is not uniform due to the large diameter and height of the fermenter, so as to avoid the problems that the traditional ultrasonic assisted fermentation device has no obvious effect on promoting fermentation in large fermenters, and the product quality is unstable.

Method used

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  • Wall-mounted ultrasonic enhanced fermentation device suitable for large fermentation tank and fermentation method of wall-mounted ultrasonic enhanced fermentation device
  • Wall-mounted ultrasonic enhanced fermentation device suitable for large fermentation tank and fermentation method of wall-mounted ultrasonic enhanced fermentation device
  • Wall-mounted ultrasonic enhanced fermentation device suitable for large fermentation tank and fermentation method of wall-mounted ultrasonic enhanced fermentation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Follow the steps above at 40m 3 The fermentation tank is used for ultrasonic enhanced fermentation of Bacillus subtilis microbial inoculum. Fermentation tank 1 is equipped with four sets of wall-mounted ultrasonic transducer brackets 2, which are evenly arranged and suspended on the inner wall. Each bracket has three sections, which are sealed by stainless steel quick-release clamps. The bracket connectors 3 are connected, and 5 ultrasonic transducers 4 are arranged longitudinally in each section. The ultrasonic transducer bracket 2 is welded by the stainless steel welding short tube 10. There are 3 ultrasonic vibrators 9 in each transducer. 5 is electrically connected to the ultrasonic generator 8;

[0058] The specific fermentation is carried out as follows:

[0059] Step 1: Add fermented liquid to fermentation tank 1. Except for water, other substrates in the fermented liquid are 1.5% (w / v, hereinafter, all those not specified refer to mass volume concentration) soy...

Embodiment 2

[0068] at 40m 3 The fermenter carries out ultrasonic-enhanced fermentation for the production of transglutaminase (TG enzyme) by fermentation of Streptomyces. Connected by stainless steel quick-release clamp seal bracket connector 3, 5 ultrasonic transducers 4 are arranged longitudinally in each section, and ultrasonic transducer bracket 2 is welded by stainless steel welding short tube 10, each transducer has 3 ultrasonic vibrators 9. Electrically connect the ultrasonic generator 8 through the high-frequency connecting wire 5 .

[0069] The specific fermentation is carried out as follows:

[0070] Step 1: Fill the fermentation tank 1 with fermentation liquid. Except for water, the other substrates of the fermentation liquid are 2.4% soybean meal hydrolyzate, 2.4% glycerol, 0.5% yeast extract, 0.3% ammonium sulfate, and the fermentation tank is packed. The capacity is 75% volume. First, sterilize the fermenter tank 1, and the sterilization conditions are 121 ° C for 15 minu...

Embodiment 3

[0078] at 40m 3 The fermentation tank is used for ultrasonic-enhanced fermentation of cordyceps sinensis to produce mycelium and polysaccharides. Fermentation tank 1 is equipped with four sets of wall-mounted ultrasonic transducer brackets 2, which are evenly arranged and suspended on the inner wall. The clamp seal bracket connector 3 is connected, and 5 ultrasonic transducers 4 are arranged longitudinally in each section. The ultrasonic transducer bracket 2 is welded by the stainless steel welding short tube 10. There are 3 ultrasonic vibrators 9 in each transducer. The frequency connecting line 5 is electrically connected to the ultrasonic generator 8 .

[0079] The specific fermentation is carried out as follows:

[0080] Step 1: Add fermentation broth to fermentation tank 1, except water, other substrates are rice bran powder 3.6%, bran powder 4.8%, KH 2 PO 4 0.27%, MgSO 4 ·7H 2O 0.27%, cysteine ​​hydrochloride 0.05%, copper chlorophyll sodium salt 0.01%, and the fer...

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Abstract

The invention belongs to the field of bioengineering equipment, and relates to a wall-mounted ultrasonic enhanced fermentation device suitable for a large fermentation tank and a fermentation method thereof, the wall-mounted ultrasonic enhanced fermentation device comprises a fermentation tank, an ultrasonic transducer bracket, a bracket connecting piece, an ultrasonic transducer, a high-frequency connecting wire, a stirring shaft, a stirring paddle, an ultrasonic generator, an ultrasonic vibrator and a stainless steel welding short pipe; the stirring shaft is arranged in the tank body and is coaxial with the fermentation tank; a stirring paddle is arranged at the other end of the stirring shaft; a plurality of ultrasonic transducer brackets are arranged in the fermentation tank in an array manner, a plurality of ultrasonic transducers are arranged on the ultrasonic transducer brackets, and a plurality of ultrasonic vibrators are arranged on the inner walls of the ultrasonic transducer brackets; and the ultrasonic vibrator is electrically connected with the ultrasonic generator through a high-frequency connecting wire. The device disclosed by the invention solves the problems that the fermentation promoting effect is not obvious and the product quality is not stable due to the fact that ultrasonic waves of a traditional ultrasonic fermentation device are easy to attenuate and non-uniform in action.

Description

technical field [0001] The invention belongs to the field of biological engineering equipment, and particularly relates to a wall-mounted ultrasonic intensified fermentation device suitable for large-scale fermentation tanks. Background technique [0002] Due to the long period of growth and reproduction of microorganisms and the low rate of material exchange between fermentation substrates and microorganisms, the fermentation period is very long and the yield of products is also low, which is an important hindering factor for bio-fermentative transformation. [0003] As a physical method, ultrasonic waves are widely used in strengthening the value-added processing of agricultural products. Ultrasound has shown outstanding application value in the extraction of active ingredients of agricultural products, food sterilization, cell disruption, homogeneous emulsification, and promotion of heat and mass transfer. Especially in the promotion of fermentation by ultrasound, it also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/02C12M1/00
CPCC12M35/04C12M39/00C12M27/02
Inventor 何荣海马海乐代春华张荣吴本刚许海宁
Owner JIANGSU UNIV
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