Application of hydroxysafflor yellow A in preparation of medicine for treating adenomyosis
A technology of adenomyosis and hydroxysafflower, applied in the field of biomedicine, can solve the problems of adenomyosis without hydroxysafflor yellow A, and achieve the effect of inhibiting collagen deposition and reducing the level
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Oral tamoxifen-induced adenomyosis model establishment:
[0022] 16 ICR mice (12 female mice, 4 male mice, 6 weeks old, body weight 18-20 g, provided by the animal room of Nanjing University School of Medicine) were caged in a male-female ratio of 1:3. Male mice were removed after conception of female mice. Newborn ICR mice were randomly divided into groups (model group n=45, control group n=10), and the mice were treated as follows on day 2-5 of birth: model group was instilled with 5 μL / g peanut oil / lecithin / condensed milk mixture ( 2: 0.2: 3, v / v / v) + 2.7 μmol / kg tamoxifen suspension, the control group was drip-fed with the same dose of vehicle mixture without drugs; From 22d onwards, the mice were separated from the females and allowed to eat and drink freely. All mice were housed in a clean-grade rat room with an air humidity of 50%-60%, a temperature of 21°C, and light / dark for 12 hours each. At the age of 42 days, the mice were randomly selected to be sacrific...
Embodiment 2
[0025] 16-week-old model mice (n=40) and blank control mice (Control, n=8) were selected, and the model mice were randomly divided into 5 groups: model group (Model), positive drug control group ( Celecoxib, celecoxib 6.5mg / kg), hydroxysafflor yellow low-dose group (HSYAL, 2.5mg / kg), hydroxysafflor yellow medium-dose group (HSYAM, 5mg / kg), hydroxysafflor yellow pigment The high-dose group (HSYAH, 10 mg / kg) was administered by intraperitoneal injection (ip). The model group and the blank control group were given the same amount of normal saline, administered daily for 3 weeks, and their body weight was measured weekly. After 3 weeks of administration, the mice were anesthetized with isoflurane, blood was collected from the retrobulbar venous plexus, centrifuged (5000g, 4°C, 15min), and the obtained mouse serum was stored at -80°C. Mice were sacrificed after blood collection, and the uterus was removed. The wet weight of the uterus was weighed, one side of the uterine horn was ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More