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Real-time pulsed electric field cell exposure system and application thereof

A pulsed electric field and cell technology, applied in the field of pulsed electric field generation systems and pulsed electric field generators, can solve the problems of difficulty in confirming the correlation of pulsed electric fields and difficulty in eliminating interference.

Pending Publication Date: 2022-07-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, it is difficult to confirm the relationship between the pulsed electric field and the cell effect, especially it is difficult to rule out the interference of other exogenous factors other than the pulsed electric field

Method used

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  • Real-time pulsed electric field cell exposure system and application thereof
  • Real-time pulsed electric field cell exposure system and application thereof
  • Real-time pulsed electric field cell exposure system and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] see figure 1 , The real-time pulsed electric field cell exposure system is composed of a pulsed electric field generator (1), a cell culture dish (2), a parallel plate electrode (3), an inverted fluorescence microscope (4), a CCD camera (5), and a camera operating system (6) , a fluorescence image processing system (7), a computer (8) and the like. The parallel plate electrode (3) is matched with the cell culture dish (2), the pulsed electric field generator (1) is connected with the parallel plate electrode (3) and the computer (8), the inverted fluorescence microscope (4) is connected with the CCD camera (5), The CCD camera (5) is connected with the camera operating system (6) and the computer (8), and the fluorescence image processing system (7) is connected with the computer (8).

[0021] see figure 2 , the parallel plate electrode (3) is composed of a metal electrode (9) and an electrode substrate (10). The electrode substrate (10) is arranged with a metal shee...

Embodiment 2

[0028] see image 3 , the pulse electric field generator (1) comprises a power supply (16), a display (17), a voltage generator (18), a voltage measurement (19), an energy storage capacitor bank (20), an energy switch bank (21), a load (22) ), a NAND gate (23), a pulse generator (24), an energy selector (25), a floating circuit (26), and the like.

[0029] The voltage generator (18) generates and regulates the output voltage by adjusting the frequency of the square wave signal, and its output N1 is connected to the input of the energy switch group (21). The energy switch group (21) uses the pulse signal of the control terminal N3 to control the conduction of the triode, so that the double-pole double-throw relay switches from the normally closed contact to the normally open contact. The contact N4 of the relay in the energy switch group (21) is connected to the energy storage capacitor group (20). There are five energy storage capacitors in the energy storage capacitor bank ...

Embodiment 3

[0037] Application of intracellular ROS in the study of real-time pulsed electric field exposure in rat hepatoma cells.

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Abstract

The real-time pulsed electric field cell exposure system consists of a pulsed electric field generator, a cell culture dish, a parallel plate electrode, an inverted fluorescence microscope, a CCD (Charge Coupled Device) camera, a camera operating system, a fluorescence image processing system, a computer and the like. The parallel plate electrode is matched with the cell culture dish, the pulsed electric field generator is connected with the parallel plate electrode and the computer, the inverted fluorescence microscope is connected with the CCD camera, the CCD camera is connected with the camera operating system and the computer, and the fluorescence image processing system is connected with the computer. Under the excitation of the pulsed electric field generator, a pulsed electric field can be generated and acts on cells between the metal electrodes, and meanwhile, the dynamic process that fluorescence in the cells changes along with time is observed and recorded. The interference of possible internal factors and external factors is eliminated, and the electromagnetic biological effect of the cells under the stimulation of the pulsed electric field is accurately judged.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a pulsed electric field generator, in particular to a pulsed electric field generation system that can observe and record the dynamic response of intracellular fluorescence to the pulsed electric field in real time under a fluorescence microscope and a system thereof. Background technique [0002] Electromagnetic biological effects are the response of organisms to electromagnetic fields or electromagnetic waves, which can be divided into thermal effects and non-thermal effects. The mechanism of thermal effect is relatively clear, which is caused by Joule heat or radiant energy. The non-thermal effect is a weak effect, and the mechanism of action is not yet clear, and it is a research hotspot of bioelectromagnetics. The biological effect of electromagnetic field starts from the original physical effect, and goes through a series of joint reactions of physical, chemical, biochemical, ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42C12M1/22
CPCC12M23/10C12M35/02
Inventor 包家立黄伟伟
Owner ZHEJIANG UNIV
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