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Tissue engineering autologous complex skin and its preparation method

A tissue engineering and autologous technology, applied in the fields of biochemical equipment and methods, tissue culture, prosthesis, etc., can solve the problems of slow absorption of stents, unfavorable wound healing, short survival period, easy breakage, etc., and achieve long tissue survival time and repair. Fast, unbreakable effect

Inactive Publication Date: 2007-01-31
吕伟光
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue-engineered skin produced by the allogeneic cells approved by the US FDA has a good clinical effect in the short term, but there are still three problems: first, the survival period is short due to the rejection of the allogeneic cells; Second, the slow absorption of the scaffold is not conducive to wound healing; third, because it is a single epithelial cell, the healed skin is very thin and easily broken, resulting in new wounds

Method used

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  • Tissue engineering autologous complex skin and its preparation method
  • Tissue engineering autologous complex skin and its preparation method
  • Tissue engineering autologous complex skin and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, preparation of tissue engineered composite skin

[0027] 1. Extraction of patient's own healthy cells and cell culture passage

[0028] (1) Extraction of patient's own healthy cells

[0029] 1. Materials obtained from the operating room

[0030] 1) Use tincture of medical iodine to clean the patient's skin surface;

[0031] 2) Rinse the leather surface with physiological saline to remove residual iodine tincture;

[0032] 3) Take a narrow and long skin of 1cm×2.5cm;

[0033] 4) Put the removed skin into the storage solution and send it to the cell culture room.

[0034] 2. Preservation of skin tissue after collection: use 5 ml of DMEM (purchased from Hyclone, USA) culture solution containing 100 units / ml penicillin and 100 mg / ml streptomycin to preserve the tissue in a preservation tube (4° C.).

[0035] 3. Sterilize and clean in an ultra-clean workbench, and separate large pieces of tissue: wash the tissue with 0.1M PBS containing 100 units / ml penici...

Embodiment 2

[0078] Embodiment 2, clinical report

[0079] 1. Inclusion criteria

[0080] 1. The wounds of patients with deep burns after incision and scab removal.

[0081] 2. Long-term non-healing ulcer wounds and granulation wounds caused by various reasons (late granulation wounds after third-degree burns, refractory ulcer wounds such as diabetic feet, etc.).

[0082] 3. Extensive scar hyperplasia, contracture deformity, or keloids, hypertrophic scars, hard, itchy, painful scars all over the body, scar removal and skin grafting are required.

[0083] Patients who belong to the above situation and are under the age of 60 are eligible for inclusion.

[0084] 2. Criteria for Excluding Cases

[0085] 1. Compound injuries such as shock period and chemical poisoning.

[0086] 2. Combined with serious primary diseases such as cardiovascular, cerebrovascular, liver, kidney, and hematopoietic system.

[0087] 3. People with allergies or autoimmune diseases.

[0088] 3. Materials

[0089]...

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Abstract

A tissue engineered auto compound skin is disclosed. The compound skin consists of auto dermal cell layer attached to the fibrin bio-stent and auto epidermic cell layer. The preparation process: amplifying and differentiating epidermis stem cell and corium fibroblast, implanting, culturing epidermis cell on the corium fibroblast containing fibrin bio-stent, and constructing tissue engineered auto-compound skin in vitro. The tissue engineered compound skin can be applied in the preparation of bio-material for skin transplantation. The advantages of the present invention lies in that it has no rejection action, highly survival rate, long tissue survival period and the wound surface can be repaired quickly with a favorable healing up effect and normal appearance.

Description

technical field [0001] The invention relates to a tissue-engineered autologous composite skin and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] Tissue engineering technology is a high-tech technology established in the world after 1980 for the preparation of transplantable tissues and organs with morphological characteristics and functions. The products currently being developed internationally include: skin, dermis , mucous membranes, tendons, bones, cartilage, blood vessels, nerves, cornea, heart valves, kidneys, glands and digestive organs, etc., and the skin tissue, dermis tissue and soft tissue were the first to succeed. Skin and dermal tissue are the first products to obtain FDA certification in 1999, including Apligraf, Integraf and Dermagraf. Since the tissue tube prepared by tissue engineering technology contains living cells and possible biodegradable materials, the product can be used on the surface of t...

Claims

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Application Information

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IPC IPC(8): A61L27/60C12N5/08
Inventor 韩斌田书彦杜彦侠张旭
Owner 吕伟光
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