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Soybean oligopeptide production process and enzyme hydrolysis reaction kettle for realizing said process

A production process, soybean oligopeptide technology, applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical instruments, etc. The effect of content, small molecular weight and light color

Inactive Publication Date: 2004-09-01
MILITARY SUPPLIES UNIV THE CHINESE PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product is yellow-white powder, and it is only in the stage of laboratory research, and has not yet formed industrialized mass production

Method used

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  • Soybean oligopeptide production process and enzyme hydrolysis reaction kettle for realizing said process
  • Soybean oligopeptide production process and enzyme hydrolysis reaction kettle for realizing said process
  • Soybean oligopeptide production process and enzyme hydrolysis reaction kettle for realizing said process

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Embodiment 1

[0022] Weigh 20 kg of soybean protein isolate, mix and dissolve it with pure water to form a slurry, add it to the enzyme hydrolysis reaction kettle, start the stirring device to stir the feed liquid, heat to 95°C, keep it warm for 30 minutes, then stop heating, and add circulating cooling water Cool the feed liquid to 40°C, slowly add 2N sodium hydroxide solution to the feed liquid until the pH value of the feed liquid is 8.0, add 2 kg of alkaline protease, and add alkali or acid dropwise according to the pH value of the acidity meter to ensure the pH value of the feed liquid 8.0 is unchanged to facilitate the smooth progress of the enzymatic hydrolysis reaction. The reaction is carried out under the conditions of constant temperature, constant PH value and constant stirring speed for 4 hours. Add 2 kg of flavor protease and continue the reaction for 5 hours. Heat to 95°C to kill the enzyme for 30 minutes. Cool down to 40°C, add 2 kg of activated carbon (200 mesh), continue to...

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Abstract

A soybean oligopeptide manufacturing process and enzyme hydrolytic reaction still for realizing the process relating to a separation and extraction process for short peptide mixture comprising the steps of, choosing raw material, hydrolyzing, centrifugal separation, and barrier separation, while the enzyme hydrolytic reaction still comprises a mixing system, an acid-alkali charging system, a pH observation system and a cool-down system.

Description

Technical field: [0001] The invention belongs to the technical field of soybean deep processing, and relates to a process for separating and extracting short peptide mixtures. Background technique: [0002] Soybean oligopeptide is a kind of soybean oligopeptide, which is made of soybean meal or soybean protein isolate. Amino acid composition, mixture of short peptides with molecular weight distribution in the range of 200-600 or 600-1000 Daltons. [0003] The research and development in the United States and Japan was earlier, and it has been mass-produced and sold. The product is a yellow-white powder, and its indicators are protein ≥ 83%, ash ≤ 6.5%, moisture ≤ 5%, sugar and other components ≤ 5.5%. [0004] There are many research units on oligopeptides in my country, such as Wuxi University of Light Industry, China Agricultural University, Zhengzhou Grain Institute, Beijing Taozheng Biotechnology Company, Hebei University of Science and Technology, Jilin University, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12P21/00
Inventor 梁歧罗敏
Owner MILITARY SUPPLIES UNIV THE CHINESE PLA
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