Glyconitroso ferrohemoglobin pigment, it preparation and application

A technology for sylation of nitroso-hemoglobin, applied in the field of glycosylated nitroso-hemoglobin pigment and its preparation and application, to achieve the effects of improved storage stability, good dispersibility, and light stability

Inactive Publication Date: 2005-08-10
上海华宝孔雀香精有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The method of glycosylation of nitrosohemoglobin to generate polysaccharide-protein covalent conjugates and improve the stability and dispersibility of nitrosohemoglobin pigment has not been reported at home and abroad.

Method used

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  • Glyconitroso ferrohemoglobin pigment, it preparation and application
  • Glyconitroso ferrohemoglobin pigment, it preparation and application
  • Glyconitroso ferrohemoglobin pigment, it preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Collect fresh pig blood qualified for quarantine, add 0.5% sodium citrate for anticoagulation, centrifuge at 3000r / min for 15min, discard the upper serum, add an equal volume of normal saline (0.9%NaCL) to wash the red blood cells, and centrifuge again at the same speed After 15 minutes, red blood cells were collected. Add an equal volume or three times the volume of distilled water, stir for 30 minutes, and then swell and break the wall to release hemoglobin.

[0031] Add 0.1% sodium erythorbate and 0.05% sodium nitrite to the hemoglobin solution, adjust the pH to 5.9, and marinate at 4°C for 24 hours to obtain a nitrosohemoglobin solution.

[0032] Nitrosohemoglobin is mixed with 1% chitosan solution (dissolved in 1% acetic acid solution) of equal volume, the concentration of nitrosohemoglobin in the reaction solution is 5%, the concentration of sugar is 0.5%, and the pH adjustment is 4.5, Then heated at 50°C for 20 minutes, and filtered to obtain glycosylated nitros...

Embodiment 2

[0035] The sugar solution used is an equal-volume mixed solution of 2% chitosan (dissolved in 1% acetic acid) and 2% glucose, the nitrosohemoglobin is mixed with the mixed sugar solution in equal volume, and the concentration of the nitrosohemoglobin in the reaction solution is 6%, the concentration of sugar is 1%, adjust the pH to be 6.0, heat 5min at 70°C, all the other operating conditions are the same as in Example 1, the sugar content in the obtained powdery pigment is 18%; its ultraviolet absorption is different from that of microcapsules Buried nitrosohemoglobin, a mixture of nitrosohemoglobin and carbohydrates. After heating at 85°C for 10 minutes, the glycosylated nitrosohemoglobin pigment solution is still a red liquid, while the microcapsule-embedded pigment has denatured and precipitated. Experiments show that the thermal stability of the glycosylated pigment is significantly improved.

Embodiment 3

[0037] Mix 2 volumes of hemoglobin solution with 1 volume of 2% chitosan solution, so that the concentration of nitrosohemoglobin in the reaction solution is 8%, the content of sugar is 0.7%, adjust the pH to 7.5, and heat at 40°C for 50min. All the other operating conditions are the same as in Example 1, and the sugar content in the obtained powdery pigment is 8%, which is lower than the product embedded in microcapsules.

[0038] Measured by differential scanning calorimetry (DSC), the thermal denaturation temperature of nitrosohemoglobin is 74.13°C, and the thermal denaturation temperature of glycosylated nitrosohemoglobin increases from 74.13°C to 97.69°C through glycosylation treatment of proteins. However, the thermal denaturation temperature of the nitrosohemoglobin pigment embedded in microcapsules was 79.19°C, indicating that the thermal stability of the glycosylated nitrosohemoglobin pigment was significantly improved.

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Abstract

A glyosylated nitroso hemoglobin pigment for food is prepared from the hemoglobin in the blood of animals or fowls through reacting on sodium nitrite or NO to obtain nitroso hemoglobin, reacting on saccharide to obtain glyosylated nitroso hemoglobin pigment and spray drying. It can be mixed with antioxidizing agent and antiaseptic to obtain a preserving system used for meat. Its advantages are high stability and dispersity, and high effect to decrease the residue of sodium nitrate in preserved meat.

Description

technical field [0001] The invention provides a glycosylated nitroso-hemoglobin pigment and its preparation and application, and relates to the use of sugars to treat nitroso-hemoglobin to produce a stable red pigment—glycosylated nitroso-hemoglobin (Glycosylated Nitroso-hemoglobin) The method, as well as its application in the coloring food processing of meat products, fruit and vegetable juice drinks, ice cream, ice cream, bread, pastry, jelly, etc., belong to the production of food additives [0002] technology field. Background technique [0003] In meat processing, nitrite can make meat products have bright color, good texture and unique flavor, so nitrite is a widely used and important coloring agent in meat curing. However, nitrite is a toxic substance (ADI0-0.06mg / kg), which can be converted into nitrosamines in the process of pickling and hair color, and become a strong carcinogen. For this reason, reducing the amount of nitrite used in the production of meat prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/40A23L29/00
Inventor 夏文水杨锡洪
Owner 上海华宝孔雀香精有限公司
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