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Process for producing fixed particle to repair surface water

A surface water and particle technology, applied in chemical instruments and methods, fixed on/in organic carriers, biological water/sewage treatment, etc. Uniformity and other problems, to achieve the effect of excellent diffusion and mass transfer performance, excellent particle elasticity, and uniform microstructure

Inactive Publication Date: 2006-03-01
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] PVA and Na Alg are two good immobilized carrier materials, which have a good embedding effect on many microorganisms, and have been widely used in the fields of food, pharmaceuticals, environmental protection, new energy development, and chemical synthesis; however, , but the traditional PVA and Na Alg embedding fixation methods still have many defects and deficiencies, such as: poor diffusion and mass transfer properties of particles, uneven pores, Na Alg self-dissolution, low mechanical strength, poor density controllability, The ability to resist temperature changes and pH changes is weak. Once it is put into surface water restoration, the immobilized particles are easily broken, the physiological activity of the bacteria is low, and the changes in hydraulic conditions and external factors have strong impact and destructive power on the particles, resulting in repair failure.
In addition, a certain traditional PVA and Na Alg immobilization method usually corresponds to a single bacterial species, lacks broad-spectrum adaptability, and is unfavorable to the immobilization of mixed bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Incline culture of mixed bacteria

[0043] Bacillus, Micrococcus, Flavobacterium and bacillus, cultured in an incubator at 28°C-30°C for 2 days.

[0044] (2) Preparation of mixed bacteria seed solution

[0045] Receive 2 loops of bacterial lawns on the slant medium with an inoculation loop respectively, and insert them into the liquid seed medium (1.25% glucose, 0.25% yeast extract, 0.1% NH 4 NO 3 , 0.02% MgSO 4 ·7H 2 O, 0.02% KCl, pH=7.0-7.2), cultured for 16-20 hours under the condition of shaker rotation speed 130rpm-140rpm, 28°C-30°C; mixed culture solution to prepare mixed bacteria seed solution.

[0046] (3) Gel solution configuration

[0047] According to the total mass of 200g, weigh 9.8% PVA powder, 0.45% Na Alg, 3.5% activated carbon powder, 3.0% zeolite powder (0.074mm content is greater than 70%), 0.08% yeast extract, soak with 40g tap water for 18 hours. Sterilize with moist heat at 111°C for 30 minutes, then cool to 39°C.

[0048] (4) Cross-lin...

Embodiment 2

[0061] The difference from Example 1 is:

[0062] When preparing the gel solution, weigh 10.8% PVA powder, 0.52% Na Alg, 4.2% activated carbon powder, 4.0% zeolite powder (0.074mm content is greater than 70%), and 0.11% yeast extract according to the total mass of 200g. Soak in tap water for 20 hours. Sterilize with moist heat at 111°C for 25 minutes and cool to 38°C. When configuring the crosslinking agent, weigh 9.25% ammonium pentaborate according to the total mass of 600 g, dissolve it in 90.75% distilled water, and adjust the pH to 6.4 to prepare the primary crosslinking agent. According to the total mass of 600g, weigh 2.6% AlCl 3 and 1.3% FeCl 3 , dissolved in 96.1% distilled water and adjusted to pH = 2.6 to obtain a secondary crosslinking agent. When preparing the reinforcement, weigh 9.1% Na according to the total mass of 600g 2 SO 4 , to prepare a reinforcing agent. When preparing immobilized granules, control the droplet velocity at the outlet by vacuum suct...

Embodiment 3

[0066] The difference from Example 1 is:

[0067] When preparing the gel solution, weigh 10.3% PVA powder, 0.48% Na.Alg, 3.8% activated carbon powder, 3.4% zeolite powder (0.074mm content is greater than 70%), and 0.10% yeast extract according to the total mass of 200g. Soak in tap water for 22 hours. Sterilize with damp heat at 111°C for 28 minutes, then cool to 41°C. When configuring the crosslinking agent, weigh 9.05% ammonium pentaborate according to the total mass of 600 g, dissolve it in 90.95% distilled water, and adjust the pH to 6.0 to prepare the primary crosslinking agent. According to the total mass of 600g, weigh 2.0% AlCl 3 and 1.1% FeCl 3 , dissolved in 96.9% distilled water and adjusted to pH = 2.4 to obtain a secondary crosslinking agent. When preparing the reinforcement, weigh 8.3% Na according to the total mass of 600g 2 SO 4 , to prepare a reinforcing agent. When preparing immobilized granules, control the outlet droplet velocity to 29 drops / min by v...

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Abstract

This invention relates to cell fixed microbial preparation, which is a kind of producing method for surface water repair immobilized particles. 1)adding active carbon ,zeolite powder or kieselguhr to premixed PVA-Naí»Alg gel solution, , as well as yeast extract paste; 2)adding gel solution with thallus seed solution,

Description

technical field [0001] The invention relates to cell immobilized biological preparations, in particular to a PVA-Na·Alg embedded and immobilized microorganism technology. Background technique [0002] The problem of surface water pollution is the product of the rapid development of modern industry and agriculture. This phenomenon is particularly prominent in industrial towns with intensive heavy and chemical industries. The phenomenon of water quality and water shortage caused by pollution is gradually intensified. More than 90% of the urban waters are seriously polluted, and the annual direct economic loss due to surface water pollution exceeds 450 billion yuan. The damage to the regional water environment leads to a continuous decline in environmental capacity, which not only seriously restricts economic development, but also causes water pollution. Disputes have caused frequent social instability, and surface water treatment and restoration has become a realistic and urge...

Claims

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Application Information

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IPC IPC(8): C12N11/04C02F3/34
Inventor 李海波李培军鞠京丽张轶许华夏尹炜
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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