High-density bacterium culturing method and its biological reactor

A technology of cell culture and bioreactor, which is applied in the field of cell high-density circulation or continuous culture method and its bioreactor device, which can solve the problem of difficult to eliminate bacteria pollution, difficult circulation or continuous high-density culture, etc. problems, to achieve the effect of improving equipment utilization, shortening production cycle, and reducing operating costs

Inactive Publication Date: 2006-05-31
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the organic membrane used in the high-density purebred culture method using an external hollow fiber membrane tube cannot be sterilized by heat, so it is difficult to el

Method used

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  • High-density bacterium culturing method and its biological reactor

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Example Embodiment

[0023] Example 1

[0024] Lactic acid bacteria circulating high-density culture

[0025] First, add beef extract 1%, peptone 1%, yeast extract 1%, glucose 15%, Tween 80 0.05% pH 6.5 medium to the cleaned bioreactor, and perform the conventional method on the bioreactor 2 and the tank If the connected pipeline is heat sterilized, it can be cultured at 30°C after being cooled by the jacket cooling water and then connected to the seed liquid. Before the end of the culture, when K2K3K4 is closed, open K1K6K8 and use steam to sterilize the inorganic membrane module and pump 4 and the pipe connected to it. Close K6K7K8 and cool to room temperature appropriately. When the concentration of lactic acid bacteria in the bioreactor reaches 10 5 cfu / ml-10 8 When cfu / ml, turn on K2K1K5, turn on pump 4, circulate and filter through the membrane tube, discard the clear liquid through the clear liquid outlet, and return the concentrated bacterial liquid to the bioreactor. After circulating and fil...

Example Embodiment

[0026] Example 2

[0027] Continuous high-density cultivation of Escherichia coli

[0028] First, add lactose 0.5%, tryptone 2%, potassium dihydrogen phosphate 0.275%, dipotassium hydrogen phosphate 0.275%, NaCl 0.5%, sodium lauryl sulfate 0.05% pH6.6- into the cleaned bioreactor. 7.0 culture medium, heat sterilize the bioreactor 2 and the pipeline communicating with the tank according to the conventional method, and then it can be cultured under certain conditions after being cooled by the jacket cooling water. Before the end of the culture, when K2K3K4 is closed, turn on K1K6K8 and use steam to sterilize the inorganic membrane module and pump 4 and the pipes connected to it. Close K6K7K8 and cool appropriately. When the E. coli concentration in the bioreactor reaches 10 5 cfu / ml-10 8 When cfu / ml, turn on K2K1K5, turn on pump 4, circulate and filter through the membrane tube, discard the clear liquid through the clear liquid outlet, and return the concentrated bacterial liquid to...

Example Embodiment

[0029] Example 3

[0030] Continuous high-density culture of yeast

[0031] First, add wort (2%-12%) into the cleaned bioreactor, heat sterilize the bioreactor 2 and the pipeline communicating with the tank according to the conventional method, and then pass the jacket cooling water to cool it. It can be cultured under certain conditions with seed liquid. Before the end of the culture, when K2K3K4 is closed, turn on K1K6K8 and use steam to sterilize the inorganic membrane module and pump 4 and the pipes connected to it. Close K6K7K8 and cool appropriately. When the yeast concentration in the bioreactor reaches 10 5 cfu / ml-10 8 When cfu / ml, turn on K2K1K5, turn on pump 4, circulate and filter through the membrane tube, discard the clear liquid through the clear liquid outlet, and return the concentrated bacterial liquid to the bioreactor. After circulating and filtering, the bacteria in the bioreactor After the concentration is increased by 5-10 times, add the sterilized and cooled...

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Abstract

A culture of high-density cell and its biological reactor are disclosed. It is characterized by installing inorganic membrane filter assembly and feed-supplement pot on biological reactor and connecting the three parts into a biological reactor system for cell circulation high-density culture or high-density continuous culture by control loop. It can combine the cell culture with membrane filter concentrate to obtain the needed metabolic product. It costs low, has short reaction time and higher production efficiency.

Description

technical field [0001] The invention relates to a cell culture method and a biological reaction device thereof, in particular to a cell high-density circulation or continuous culture method and a biological reaction device thereof. Background technique [0002] Membrane bioreactor is a new type of equipment formed by introducing membrane separation technology into bioreactor. The existing membrane bioreactor (MBA) is mainly used for sewage treatment. Its working principle is to use membrane to intercept the activated sludge In order to improve the efficiency of sewage biological treatment by microorganisms, the core problem is to solve the problem of membrane filtration pollution, that is, the decrease of filtration flux in the process of sewage biological treatment. Since the biological sewage treatment is an open fermentation process, it does not involve the pollution of bacteria, that is, the membrane equipment used in this type of bioreactor does not need to consider the...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N5/00
Inventor 熊晓辉熊强陆利霞韩亦龙
Owner NANJING UNIV OF TECH
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