Method of improving PHA synthesis yield of residual active sludge by native PHA synthesis bacteria refilling process

An activated sludge, native high-yield technology, applied in water/sludge/sewage treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of restricting popularization and application, low yield, etc., and reduce production costs , increase yield, inhibit growth effect

Inactive Publication Date: 2006-06-14
NANKAI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yield of PHA synthesized from residual activated sludge is not high, which restricts the popularization and application of this method.

Method used

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  • Method of improving PHA synthesis yield of residual active sludge by native PHA synthesis bacteria refilling process
  • Method of improving PHA synthesis yield of residual active sludge by native PHA synthesis bacteria refilling process
  • Method of improving PHA synthesis yield of residual active sludge by native PHA synthesis bacteria refilling process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Using reinjection single high-yield native PHA synthetic bacteria to increase the yield of PHA synthesis in sludge (activated sludge in aerobic pool of domestic and a small amount of industrial wastewater treatment plant).

[0020] 1. Test equipment

[0021] 1.1 Biological reaction tank

[0022] This test uses a 3-liter self-made reaction barrel as a biological reaction barrel. The top of the reaction barrel has a liquid inlet, a liquid outlet, an exhaust hole and a thermometer hole, and a built-in magnetic rotor. The biological reaction barrel is placed in a constant temperature water bath with magnetic stirring function. inside the device to ensure a constant temperature during the reaction.

[0023] 1.2 Gas supply system

[0024] The air pump used in this test can accurately control the amount passed into the reaction barrel to be 1.2L / min.

[0025] 1.3 Drainage and replenishment constant flow pump

[0026] The constant-flow pump can absorb liquid at a uniform sp...

Embodiment 2

[0065] Using reinjection mixed with high-yielding indigenous PHA synthetic flora to increase the yield of PHA synthesis in sludge (activated sludge in aerobic pools of domestic and industrial wastewater treatment plants).

[0066] 1. Test equipment

[0067] Same as Example 1

[0068] 2. Procedure

[0069] 2.1 The preparation of acclimatization liquid is the same as that in Example 1

[0070] 2.2 Fermentation broth preparation is the same as in Example 1

[0071] 2.3 PHA synthetic bacteria isolation and counting medium preparation is the same as in Example 1

[0072] 2.4 The preparation of the culture medium for counting the total number of bacteria is the same as in Example 1

[0073] 2.5 Adopt the Nile-Red method to isolate and obtain three strains of high-yielding indigenous PHA synthetic bacteria. The method is the same as in Example 1

[0074] 2.6 Identification of high-yielding indigenous PHA-synthesizing bacteria: the three isolates of high-yielding indigenous PHA-s...

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Abstract

The invention relates to a acclimation direction adopted recycling small amount aborigine PHA high yield composition mushroom to do í«gage-restrictingí» acclimation primary activity sludge, force PHA composition mushroom to form dominance flora, and increase activity sludge composition PHA yield. Its purpose is to set aborigine PHA high yield composition recycling method, supply a simple activity sludge acclimation setting. Its operation steps are adopting Nile-Red fluorescent staining method to separate the PHA composition mushroom from the activity sludge; scalping PHA high yield mushroom as the recycling mushroom; its 0.1-0.25g are inoculate in reaction barrel to finish acclimation periods; discharging acclimation fluid; adding fermentation medium and microelement to do PHA ferment; collecting sludge deposition, drying, using chloroform to extract PHA; condensing extracted fluid in methanol to cement out PHA. This method can effectively increase activity sludge composition PHA yield from thirty percent to fifty percent.

Description

technical field [0001] The invention belongs to the research field of biologically synthesized biodegradable materials Background technique [0002] Traditional plastics cannot be degraded in the natural environment, causing serious "white pollution". Therefore, all countries in the world attach great importance to the research and development of biodegradable materials. In recent years, many new biodegradable materials have been prepared by chemical synthesis and microbial fermentation. Polyhydroxyalkanoate (PHA) is an intracellular carbon source or energy storage material accumulated by many prokaryotic microorganisms under the condition of unbalanced growth, such as nitrogen or phosphorus deficiency, because PHA can be completely degraded into carbon dioxide and Water, and being thermoplastic and biocompatible, has become a research hotspot. However, due to the high production cost of PHA synthesized by pure culture fermentation, its popularization and application are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C02F3/12C12M1/36C12P7/64
CPCY02W10/10
Inventor 宋存江曾猛陶剑郑承刚金映虹邓飞王淑芳孙亚民
Owner NANKAI UNIV
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