Improved method for quick detection of Ramaria root spore density

A technology for rapid determination of arbuscular mycorrhizal fungi, applied in the determination/inspection of microorganisms, biochemical equipment and methods, preparation of test samples, etc. problem, to achieve the effect of easy identification and recognition, easy recognition, improved accuracy and speed

Inactive Publication Date: 2007-06-13
CHINA UNIV OF MINING & TECH (BEIJING)
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AI Technical Summary

Problems solved by technology

What these methods have in common is that they can enrich the mycorrhizal spores from the growth substrate, but the spores are similar to the small particles of the substrate, especially the sand grains, so it is not easy to distinguish
Th...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: (microbial agent culture substrate)

[0018] Culture medium: collected from construction sand in the north beach of Beijing, sterilized by high-temperature and high-pressure steam (121°C, 2h) before potting, and naturally air-dried for potting experiments.

[0019] For the test species: fungal strains G.mosseae, G.versiforme and G.etunicatum

[0020] Provided by the Department of Microbiology, Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.

[0021] Test crops: corn and sorghum were provided by Zhongnong Seed Company, Chinese Academy of Agricultural Sciences. Seeds were sprayed with 10% H before sowing 2 o 2 Soak for 10 minutes, rinse with deionized water several times and drain for later use.

[0022] Test pot: the test pot is a white plastic pot with a size of 20cm×20cm×15cm (height×diameter of the mouth of the basin×diameter of the bottom of the basin). After the pots were washed and drained with ta...

Embodiment 2

[0026] Embodiment 2: (the field application of bacterial agent in the ecological reconstruction of coal gangue hills in Ningxia)

[0027] Experimental site

[0028] The coal gangue hill of Dawukou Coal Washing Plant in Ningxia is located in Shizuishan City, Ningxia Hui Autonomous Region. It belongs to the arid climate zone in the middle temperate zone. The precipitation is scarce and concentrated, the sunlight is sufficient, the evaporation is strong, and the air is dry, which is extremely unfavorable for the vegetation restoration of the coal gangue hill. The test site is selected on the coal gangue hill, and the river sand with a thickness of 0.8-1m is spread on the coal gangue hill as the growth substrate. The pH of the sandy soil is 8.12, the electrical conductivity EC is 6.67μS / cm, the maximum water holding capacity is 14.48%, the available phosphorus is 2.9ppm, the available nitrogen is 5.27ppm, and the available potassium is 97ppm. It belongs to extremely barren sandy s...

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Abstract

The invention relates to mycorhiza biotechnology that is a new method of ecological management for coal field. Mycorhiza spore density is a standard to measure mycorhiza annidation. It adopts optimized method--wet sieve decantation decoration method to test spore density. It could improve testing accuracy and speed, and lowers the human error in spore density testing process. It supplies a spore density rapid testing method for ecological application of mycorhiza biotechnology.

Description

technical field [0001] The invention relates to a method for measuring spore density in microbial mycorrhizal mycology, in particular to a method for rapidly measuring the spore density of arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal fungi (AM) are ubiquitous soil microorganisms in nature. More than 90% of the flowering plants on land can form mycorrhizal symbionts with it, and mycorrhizae have obvious ecological effects on the ecological restoration of coal mining areas. Arbuscular mycorrhizal fungi are composed of mycorrhizal spores (fruits), arbuscular bodies, vesicles, and hyphae, all of which can be used as propagules. The spore is not only the nutrient storage organ of arbuscular mycorrhizal fungi, but also a stable propagule. Its morphological characteristics are still an important basis for the taxonomy of arbuscular mycorrhizal fungi. Using the spores of arbuscular mycorrhizal fungi as the inoculum source can not only obtain a...

Claims

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Application Information

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IPC IPC(8): G01N15/00G01N1/34C12Q1/02
Inventor 毕银丽吴王燕全文智
Owner CHINA UNIV OF MINING & TECH (BEIJING)
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