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Bioimmune-aggressin composition for suppression of Xanthomonad infections in agriculture crops

a technology of xanthomonad and composition, applied in the direction of fungicides, plant/algae/fungi/lichens ingredients, biocide, etc., can solve the problems of limiting the use of such sprays, affecting the health of citrus grove owners, and affecting the quality of citrus groves,

Inactive Publication Date: 2006-07-27
BIOSYS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"This patent describes a new invention that uses dried extracts from certain plants to create a biological control agent that protects plants from harmful bacteria. The extracts can be applied as a spray, drip, or seed treatment to provide natural protection against infections in a variety of plants, such as tomatoes, citrus, carrots, and beans. The invention is designed to be safe and effective, and uses natural products from living organisms to control phytopathogens and reduce damage to agricultural crops."

Problems solved by technology

Many Xanthomonas spp. are known to be phytopathogenic, and can cause devastating agriculture diseases such as citrus canker in a variety of Citrus spp.
Thus—not being delimited by theory—the principle mode of invasion of such organisms may be primarily due through injured areas of the plants.
However, the use of such sprays is delimiting due to their toxicity.
Nonetheless, control measures have had limited success and are very expensive to both regulatory bodies and citrus grove owners.
To date, such organisms have not been employed in fighting xanthomonad-related diseases.
Such compounds have demonstrated limited success in controlling X. axonopodis pv.
Direct application of riboflavin as an elicitor for systemic resistance has been limited to laboratory experiments.
However, such controversy revolves around passive mechanical protection of plants.
However its field application is currently unresolved, and in principle it is directed toward fungal plant diseases associated with the rhizosphere.
A major problem in using this organism is based on the stability of its formulation; whereby, the viability of this organism is affected.
Nevertheless, a paucity exists within the literature regarding its applications to phyllosphere pathogens—in particular toward pathogens belonging to the genus Xanthomonas.
A major problem with the use of many Trichoderma spp. is that they produce phytotoxins which can injure targeted host plants.

Method used

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  • Bioimmune-aggressin composition for suppression of Xanthomonad infections in agriculture crops

Examples

Experimental program
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Effect test

example 1

In Vitro Screening of Hypersensitivity Responses

[0079] Hypersensitivity responses toward this invention and challenges of pathogens were performed on Citrus spp. protoplasts. Callus protoplast were derived from shoots of Citrus sinensis, C. paradisis, and C. limon and routinely prepared in our laboratory following established procedures [Kyte, L and Kleyn, J (eds.) 1996. Plants from test tubes: An introduction to microporpagation. 3rd Edition. Timber Press: Portland, Oreg.]. Callus-derived cells derived from explanted material of desired Citrus spp., and protoplast are formed from the callus-cell cultures following digestion with 3% cellulase; 0.5% macerase in solution of 0.5 M mannitol and sucrose having a final osmotic pressure of 825 m mole / kg. Recovered protoplast were grown in KM8p protoplast medium.

[0080] Freshly isolated protoplast (2O2) from protoplast as positive and negative controls. Hydrogen peroxide production was measured in the culture medium of experimental and con...

example ii

Control of Xanthomonad-Induced Leaf Spot Disease of Nepeta cataria

[0086] Catnip (Nepeta cataria) is subject to leaf spot disease by a variety of bacteria including X. campestris. In order to test the effectiveness of our admixture on xanthomonad-disease, we used a catnip model. In a controlled green house located in Willis, Tex., we planted 28, 2 month old Neptea cafaria in pots. The plants were divided into experimental and control groups each having 4 plants per group. Forty-eight hours prior to experimentation, the groups were placed in saturated dew chambers.

[0087]Xanthamonas campestris was grown under agitation in nutrient broth for 48 hours, and diluted to a suspension of 106 CFU / mL as determined by plating methods. Tween 20 was added the bacterial suspension at a final concentration of 0.5%. Using a hand-held mister, plants were sprayed with the bacterial mixture. Plants were monitored every other day for evidence of disease.

[0088] Within two weeks, small brown speaks char...

example 3

Control of Xanthomonad Killing of Poa Annua

[0095] In addition to testing the effects of xanthomonad infection of catnip, we utilized the annual blue grass, Poa annua, to test the effects of our admixture on this grass' infection with Xanthomonas campestris [pv.]poannua (obtained as XPO from Eco Soil Systems). In this study, Poa annua were grown in open Phytatray (Sigma). The trays were separated into controls (N=10 trays) and experimental groups (N=10 trays). After one month of growth, experimental trays were sprayed with X. campestris [pv.]poannua according to manufacture's directions. Following infection, we waited for the appearance of injury to the grass, then sprayed them with Formula V as given above. Controls received either no Formula V or SS-9 only. Results are presented in Table 7.

[0096] As shown in Table 7, Formula V protected the grasses from progression of the disease. The effect on grass expressing minimal injury were afforded protection within one week after sprayin...

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PUM

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Abstract

A composition for treating Xanthomonas spp. infections in agriculture crops wherein the treatment composition is an admixture of T. harzianum, Y. schidigera root extract, Y. schidigera leaf extract, and X. spp. extract. The composition is also efficacious as a pre-infection agent for enhancing the resistance of agriculture crops to such infections. The composition is preferably applied by spraying.

Description

FIELD OF INVENTION [0001] The present invention concerns a compounded aggressin derived from a bioextract mixture for controlling and preventing phytopathogenic xanthomonad infections in agriculture related crops. This invention is based on the principles of inducing resistance against phytopathogenic xanthomonads using a combined mixture of bioextracts from non-phytopathogenic Xanthomonas spp., Trichoderma harzianum, and from the plant Yucca schidigera. The method is environmentally safe and affords plant protection against a variety of Xanthomonas spp. and their pathovariants. BACKGROUND OF THE INVENTION [0002] The genus Xanthomonas Dowson 1939 compromises a diverse group of gram-negative, obligate aerobic, non-fermentative, rods which are motile by a single polar flagellum, and can produce a brominated yellow pigment identified as xanthomonadin. All reported strains of this genus have been described as plant associated, and most are reported as being pathogenic to a particular pl...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N65/00A01N25/26A01N63/38A01N65/42A01P3/00
CPCA01N65/00A01N63/04A01N63/02A01N63/00A01N2300/00A01N65/42A01N63/38A01N25/06
Inventor HYSMITH, ROBERT M.
Owner BIOSYS
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