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Cleaning compositions comprising transglucosidase

a technology of transglucosidase and cleaning composition, which is applied in the direction of detergent compounding agents, dry cleaning apparatus for textiles, enzymology, etc., can solve the problems of many stains that are difficult to remove, and achieve the effect of efficient removal

Inactive Publication Date: 2008-09-25
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]In certain cases, use of the subject method results in more efficient removal of stains that contain natural gum polysaccharides than equivalent methods that do not employ a transglucosidase enzyme.

Problems solved by technology

Despite the complexity of current detergents, there are many stains that are difficult to remove.

Method used

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  • Cleaning compositions comprising transglucosidase
  • Cleaning compositions comprising transglucosidase
  • Cleaning compositions comprising transglucosidase

Examples

Experimental program
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Effect test

example 1

Expression of A. niger Transglucosidase in T. reesei

[0092]A nucleic acid encoding the mature transglucosidase enzyme of A. niger protein was amplified from the genomic DNA of A. niger by PCR and cloned into the vector pTrex3 to make pTrex3(AGL51M). pTrex3(AGL51M) is illustrated in FIG. 1. The transglucosidase protein encoded by this vector is operably linked to the CBH1 signal sequence to facilitate its secretion into the growth medium. The transglucosidase coding sequence is flanked by the T. reesei cbh1 promoter and terminator. The nucleotide sequence of the expression cassette of pTrex3(AGL51M) vector is set forth in FIG. 2.

[0093]The 7.57 kb XbaI-XbaI fragment of the plasmid pTrex3(AGL51M) was purified by agarose gel electrophoresis and used to transform the spores of the T. reesei Morph 1.1. pyr+ strain by electroporation. The electroporation parameters were as follows: voltage—16 kV / cm, capacitance—25 μF, resistance—50Ω. Electroporation was carried out using a suspension of fr...

example 2

Transglucosidase Degrades Xanthan Gum

[0096]Hydrolytic activity by enzymes on xanthan gum was measured by the reducing sugar assay using the PAHBAH (para-hydroxybenzoic acid hydrazide) reagent (Lever et al, Anal. Biochem. 1972 47: 273). Xanthan gum (CAS 111 38-66-2) was purchased from Sigma Chemicals, St. Louis Mo. and dissolved in 50 mM sodium acetate buffer pH 6.0 at a concentration of 0.2%. For some experiments AATCC standard heavy duty liquid detergent (AATCC HDL 2003 without brightner, Test Fabrics, Inc. West Pittston, Pa.) was added at 1.5 ml per liter (0.15%). The AATCC HDL liquid detergent contained 12% linear alkyl sulfonates, 8% alcohol ethoxylates, 8% propanediol, 1.2% citric acid, 4% fatty acid and 4% sodium hydroxide with the balance being water.

[0097]The assay was performed as follows in a 24 well microplate (COSTAR 3526, Corning Incorporated, Corning, N.Y.): one ml of buffer was added to well 1, one ml of buffer plus enzyme was added to well 2, one ml of buffer and sub...

example 3

Transglucosidase Removes Soil from Stained Patches

[0101]Salad dressing with pigment (STC CFT CS-6) and guar-pigment (STC CFT CS-43) soiled cotton swatches were obtained from Test Fabrics, Inc. West Pittston, Pa., USA. Swatches for the microplate assay were cut into 15 mm circles (disks) with textile Punch Press Model B equipped with a ⅝″ die cutter. Single swatch disks were placed into each well of a 24-well microplate (Costar 3526). One (1) ml of washing solution, containing per liter, 1.5 ml AATCC HDL detergent, 50 mM Hepes buffer, and 6 to 60 ppm enzyme diluted in 50 mM Hepes buffer pH 7.4 was added to each well. The microplate was covered with a plastic lid and aluminum foil and incubated at 37° C. with 100 rpm gentle rotation for 4-16 hr. The plates were removed from the shaker and the detergent solution was removed by aspiration. Each microplate well was washed three (3) times with 1.5 ml of Dulbecco's PBS pH 7.3 and three (3) times with 1.5 ml of distilled water. Each disk wa...

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Abstract

Provided herein is a composition comprising: a) a transglucosidase enzyme; and b) a natural gum polysaccharide, wherein said natural gum polysaccharide is a substrate for said transglucosidase enzyme. A method of using a transglucosidase enzyme to a degrade natural gum polysaccharide is also provided. The composition and method may be employed in cleaning applications.

Description

BACKGROUND[0001]Detergent and other cleaning compositions often include a complex combination of active ingredients. For example, certain cleaning products contain a surfactant system, enzymes for cleaning, bleaching agents, builders, suds suppressors, soil-suspending agents, soil-release agents, optical brighteners, softening agents, dispersants, dye transfer inhibition compounds, abrasives, bactericides, and perfumes. Despite the complexity of current detergents, there are many stains that are difficult to remove.[0002]This disclosure relates to the use of transglucosidase as a cleaning agent.SUMMARY OF THE INVENTION[0003]Certain aspects of this disclosure relate to a composition comprising: a) a transglucosidase enzyme; and b) a natural gum polysaccharide; wherein the natural gum polysaccharide is a substrate for the transglucosidase enzyme. The natural gum polysaccharide may be, for example, a xanthan or guar gum. The natural gum polysaccharide is present as a stain on an object...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): D06L1/02D06L1/14
CPCC11D3/222C11D3/38636D06L3/11D06L1/14C12N9/107D06L4/40C11D3/22C11D3/386
Inventor POULOSE, AYROOKARAN J.MCDONALD, HUGH C.SHETTY, JAYARAMA K.
Owner DANISCO US INC
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