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Solution for tissue adhesion prevention and method for tissue adhesion prevention

a tissue adhesion and prevention technology, applied in the direction of antinoxious agents, drug compositions, aerosol delivery, etc., can solve the problems of tissue damage, serious complications, damage, etc., to prevent tissue dehydration, prevent lipid peroxidation and lipid degradation, and inhibit oxidative stress

Inactive Publication Date: 2009-04-16
THE UNIV OF TOKYO +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides a solution for tissue adhesion prevention and a method for tissue adhesion prevention using trehalose and glycosyl-L-ascorbic acid. The solution includes trehalose and glycosyl-L-ascorbic acid, and may also include other ingredients such as antioxidants, chelates, antiseptics, hemostatics, anti-inflammatory agents, and polysaccharides. The solution prevents dehydration and oxidative stress, reduces production of free radicals, and protects tissues from mechanical damage during surgery. It can be applied to tissues including wounds and organs during surgery or treatment of wounds. The invention provides a more effective solution for tissue adhesion prevention and delay of wound cure.

Problems solved by technology

It is well known that tissues including organs are exposed to air due to incision in surgery, and these tissues can be dehydrated and oxidized leading to their damages.
The dehydration and oxidation of tissue including organs during surgery lead to their damages, which are considered as a cause of problems including postoperative tissue adhesion and delay of cure.
Since adhesion especially often inhibits normal movement of tissues including organs, it is considered as one of serious complications after surgery.
For instance, the adhesion occurred during tendon surgery may cause dyskinesia.
In addition, the organ adhesion after intra-abdominal surgery may cause complications such as ileus, pain and sterility, and sometimes can cause problems such that next surgery becomes very difficult.
However, problems has raised such that in case of perfusion of saline solution, it is difficult to maintain a sufficient covering condition of organs with fluid layer of saline solution preventing from exposure milieu that can cause oxidative stress, and in case of cover with gauze soaked with saline solution, the gauze may interfere surgery operations.
It was difficult to maintain conditions in which whole surface of exposed tissue including organs can be blocked from air during surgery.
However, the previous drugs described above lacked operationality such as user-friendliness, because their component having adhesion preventive function is macromolecule and their forms are mainly film that is placed on intended section or viscous fluid that is applied on intended section.
However, the fact that by applying protection solution using trehalose administered to tissues exposed to air including wounds and organs due to incision in operations such as surgeries, adhesion of wounds and tissue as well as delay of cure will be prevented has not been known yet.

Method used

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  • Solution for tissue adhesion prevention and method for tissue adhesion prevention
  • Solution for tissue adhesion prevention and method for tissue adhesion prevention
  • Solution for tissue adhesion prevention and method for tissue adhesion prevention

Examples

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example 1

[0108]As cells, HepG2 cells (human hepatoma-derived cell lines) were used.

[0109]For all reagents other than HepG2 cells, AA-2G, trehalose, DMEM media, FCS, neutral red, reagent grade chemicals were used. In addition, as sample solution in which HepG2 cells were activated, 4 kinds of solution; PBS (+) solution, 2.5 mM AA-2G solution, 132 mM trehalose solution, 2.5 mM AA-2G+132 mM trehalose mixed solution were prepared.

[0110]A reagent ascorbic acid-2-glucoside (AA2G; Hayashihara Biochemical Labs., Inc.) was dissolved in distilled water to obtain 2.5 mM AA-2G solution. And a highly purified hydrated crystal trehalose in powder (Reagent trehalose; Hayashihara Biochemical Labs., Inc.) was dissolved in distilled water to obtain 132 mM trehalose solution. The reagents ascorbic acid-2-glucoside and the highly purified hydrated crystal trehalose in powder were dissolved in distilled water to obtain 2.5 mM AA-2G+132 mM trehalose mixed solution. Then, pH and osmolarity of these 4 kinds of samp...

example 2

[0123]In this example, abdominal adhesion model was prepared using rabbits by desiccating surface of intestines during abdominal surgery, and the preventive effect of intestine adhesion by trehalose was discussed.

[0124]As trehalose, powder trehalose (Reagent trehalose; Hayashihara Biochemical Labs., Inc.) and +NaCl were dissolved in sterile distilled water of 250 mL as 2% solution, sterilized by filtration with filter (Millex; Millpore Co., Bedford) and poured to sterile container and stored at room temperature. As test animals, five female New Zealand White rabbits weighing 2.85 to 3.2 kg aged of 14 weeks (Std:NZW, Japan SLC, Inc., Shizuoka) were used. The rabbits were kept in animal laboratory (room temperature of 24+ / −1 degrees C., humidity of 30 to 70%) in The University of Tokyo, Graduate School of Agricultural and Life Sciences Veterinary Medical Center. The rabbits were acclimatized for 1 week after purchase, and during this period blood test (complete blood count, BUN, Cre, ...

example 3

[0132]In this example, abdominal adhesion model was prepared using rabbits by desiccating surface of intestines during abdominal surgery, and the preventive effect on intestine adhesion by trehalose was discussed.

[0133]As trehalose, powder trehalose (Reagent trehalose; Hayashihara Biochemical Labs., Inc.) were dissolved in sterile distilled water of 250 mL as 7% solution, sterilized by filtration with filter (Millex; Millpore Co., Bed ford) and poured to sterile container and stored at room temperature.

[0134]As test animals, 20 female New Zealand White rabbits weighing 2.6 to 3.2 kg aged of 14 weeks (Std:NZW, Japan SLC, Inc., Shizuoka) were used. The rabbits were kept in animal laboratory (room temperature of 24+ / −1 degrees C., humidity of 30 to 70%) in The University of Tokyo, Graduate School of Agricultural and Life Sciences Veterinary Medical Center. The rabbits were acclimatized for 1 week after purchase, and during this period blood test (complete blood count, BUN, Cre, ALT, AL...

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Abstract

The objective of the invention is to provide a solution for tissue adhesion prevention and a method for tissue adhesion prevention that are applicable to general surgery and in which covering condition during surgery is stable and convenient. The invention is the solution for tissue adhesion prevention of which the active ingredient is trehalose. Also, it contains at least one or more among antioxidants, chelates, antiseptics, hemostatics, anti-inflammatory agents, and polysaccharides, mucopolysaccharides, salts of polysaccharides and salts of mucopolysaccharides having lubricating properties. This solution for tissue adhesion prevention is provided as any form of perfusion fluid, spray fluid, solution for spray or vaporization administration, foam-like aerosol preparation, injection solution for intravenous fluids, intravenous fluid.

Description

FIELD OF THE INVENTION[0001]This invention relates to a solution for tissue adhesion prevention and a method for tissue adhesion prevention, especially to the solution for tissue adhesion prevention and the method for tissue adhesion prevention composed of trehalose, and that prevents dehydration and oxidation upon exposure to air associated with surgery, and that can prevent complications such as tissue adhesion including postoperative organs.BACKGROUND ART[0002]It is well known that tissues including organs are exposed to air due to incision in surgery, and these tissues can be dehydrated and oxidized leading to their damages. The dehydration and oxidation of tissue including organs during surgery lead to their damages, which are considered as a cause of problems including postoperative tissue adhesion and delay of cure.[0003]Since adhesion especially often inhibits normal movement of tissues including organs, it is considered as one of serious complications after surgery. For ins...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K9/12C13K13/00A61K31/715
CPCA61L31/042A61K31/7016A61P39/06A61P41/00A61K31/375A61K9/08
Inventor SUZUKI, SHIGEKIMIWA, YOSHIKATSUSASAKI, NOBUOTEI, YUIICHI
Owner THE UNIV OF TOKYO
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