Smart, Self-Decontaminating Polymer and Method for Inhibiting The Growth Of A Bacteria and Fungus
a technology of antimicrobial system and polymer, which is applied in the direction of biocide, bandages, peptide/protein ingredients, etc., can solve the problems of argyria or silver toxicity, the risk of silver containing products for wound treatment, and the danger of silver toxicity,
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example 1
Synthesis of a Responsive Hydrogel
[0056]In this embodiment of the present invention, a film was selected as the application method of choice, and the formulation was optimized. The final formulation comprises five components: PEG (polyethylene glycol) diacrylate (22 wt %), PEG 10,000 g / mol (33 wt %), a UV (ultraviolet) initiator (10 wt %), a buffer (35 wt %), and modified chloroperoxidase and glucose oxidase enzymes (0.002%). Polyethylene glycol diacrylate (220 mg−molecular weight of 3,400 g / mol) and linear polyethylene glycol (330 mg−10,000 MW) are first added to 50 mM sodium phosphate solution (pH=8.0). Then, 100 μL of 15 wt % 2-hydroxy-1-[4-(2-hydrozyethoxy)phenyl]-2-methyl-1-propanone in ethanol is also added followed by the addition of glucose oxidase (40 units / mL final volume) and chloroperoxidase (20 units / mL final volume). A small volume (100 μL) of the solution was placed between two 25 mm diameter glass cover slips. The sample was then photopolymerized into a gel by exposi...
example 2
Synthesis of a Biogenic Amine Responsive Urethane
[0057]A predetermined unit activity of Monoamine Oxidase, Diamine Oxidase, and Peroxidase (1, 75, 108) is mixed together and dissolved in a sufficient amount of Potassium Phosphate Buffer pH 7.5 (50 mM) (See reactions in FIGS. 8 & 9). Unit activity of monoamine oxidase is defined herein as one unit of monoamine oxidase as the amount of enzyme that will oxidize one μmole of tyramine to p-hydroxyphenylacetaldehyde and hydrogen peroxide per minute at pH 7.5 at 37° C. under the specific assay conditions. Unit activity of diamine oxidase is defined as the amount of enzyme that will cause the formation of 1 μmole of hydrogen peroxide from putrescine per minute at pH 8.0 at 30° C. under the specific assay conditions. Unit activity of peroxidase is defined as the amount of enzyme that will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20° C. under the specific assay conditions. The resulting polymer produces chlorine in res...
example 3
Chlorination Response of Tailored Biopolymer to Glucose
[0063]The chlorinating activity of composite polymers was measured by following the chlorination of a colored substrate (thionin). The thionin is a blue-violet dye that absorbs at 598 nm and undergoes bleaching (to colorless) upon chlorination. A battery of tests were carried out in 100 mM phosphate citrate buffer at pH 4.0. The reactions employed the following reagents: 0.025 mM thionin and 20 mM NaCl (in citrate phosphate buffer), 0.3% v / v hydrogen peroxide (in deionized water), 18% w / v β-D-glucose (in deionized water), 20 Units / mL of chloroperoxidase in citrate phosphate buffer (where one unit of chloroperoxidase will catalyze the conversion of 1 umole monochlorodimedon to dichlorodimedone per minute at pH 2.75 and 25° C.) and 40 Units / mL of glucose oxidase (where one unit of glucose oxidase will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and hydrogen peroxide per minute at pH 5.1 at 35° C.). The reaction mixture co...
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