4 results about "Dermatophyton" patented technology

The disclosure generally relates to cocrystals of posaconazole and a coformer. The disclosure further relates to pharmaceutical compositions comprising the cocrystals, as well as methods of making the cocrystals, and methods of treating or preventing fungal, yeast, or dermatophyte infections using the cocrystals.

The present application belongs to the technical field of detection reagents, and particularly relates to a dermatophyte detection kit based on a recombinant LGBP-FITC fluorescent probe and application thereof. The present application obtains LGBP-Cys protein by performing C-terminal cysteine site-directed mutation on LGBP protein from Procambarus clarkii; then, the LGBP-Cys protein is directionally coupled with maleimide-activated FITC under mild neutral conditions, so that the problem of protein activity damage caused by traditional random coupling is solved; finally, the coupling product is placed in a composite stabilizing solution containing an anti-quenching agent, so that the stability of the probe and the detection signal-to-noise ratio are significantly improved. The kit is simple to operate, and detection can be completed within 15 minutes. The kit has high sensitivity, high specificity and visualization advantages for common clinical dermatophytes, and is suitable for rapid bedside diagnosis.

The invention discloses a primer, a probe, an implementation method and a detection system for qPCR detection of SQLE drug-resistant mutation of dermatophytes, and the method comprises the following steps: identifying a plurality of key mutation sites related to drug resistance by comparing an obtained dermatophytes SQLE gene sequence with a reference sequence in an NCBI database; designing a specific primer, a TaqMan-MGB fluorescent probe and a wild type closed probe modified by the MGB probe based on the mutation site, and verifying the sensitivity and the specificity through the strain level; the method comprises the following steps: constructing a mixed DNA sample model containing drug-resistant strain DNA, wild type strain DNA and human DNA, optimizing a reaction system of single and multiple qPCR detection, and determining an optimal reaction system of qPCR detection. According to the specific primer and probe for qPCR detection of dermatophyte SQLE drug-resistant mutation with specificity meeting requirements and an optimized reaction system for qPCR detection, whether dermatophyte SQLE drug-resistant mutation exists or not can be quickly verified, the sensitivity and specificity of dermatophyte drug-resistant detection are improved, early precise treatment is facilitated, and prognosis of patients is improved.

This invention provides a compound called *Paris polyphylla* saponin A, its pharmaceutical composition, preparation method, and application, belonging to the fields of pharmaceutical technology and cosmetic technology. It provides *Paris polyphylla* saponin A, as well as *Paris polyphylla* saponins A-D and *Paris polyphylla* saponin A in the preparation of drugs for the prevention and treatment of superficial fungal infections of the skin. The above compounds are effective against *Trichophyton rubrum* (…). Trichophyton rubrum Epidermophyton floccosum ( Epidermophyton floccosum ) and Microsporum gypseum ( Microsporum gypseum It has a significant inhibitory effect on dermatophytes such as dermatophytes and can be used to prepare drugs for the prevention and treatment of superficial fungal infections of the skin.