1 results about "Dermatophyton" patented technology

The present application belongs to the technical field of detection reagents, and particularly relates to a dermatophyte detection kit based on a recombinant LGBP-FITC fluorescent probe and application thereof. The present application obtains LGBP-Cys protein by performing C-terminal cysteine site-directed mutation on LGBP protein from Procambarus clarkii; then, the LGBP-Cys protein is directionally coupled with maleimide-activated FITC under mild neutral conditions, so that the problem of protein activity damage caused by traditional random coupling is solved; finally, the coupling product is placed in a composite stabilizing solution containing an anti-quenching agent, so that the stability of the probe and the detection signal-to-noise ratio are significantly improved. The kit is simple to operate, and detection can be completed within 15 minutes. The kit has high sensitivity, high specificity and visualization advantages for common clinical dermatophytes, and is suitable for rapid bedside diagnosis.