Method of extracting carotenoid and edible glycerol from Dunaliella sallina

A technology of carotene and Dunaliella salina, which is applied in the fields of edible oil/fat, application, food science, etc., can solve the problems of affecting the recycling of culture medium, strict quality deviation requirements, and high collection costs, so as to achieve less damage to algae, High economic value, environmentally friendly effect

Inactive Publication Date: 2009-09-09
TSINGHUA UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the current concentration and collection methods include high-speed centrifugation, flocculation and precipitation, hydrophobic adsorption, alkaline flocculation and air flotation, etc. Among them, the direct use of high-speed centrifugation technology has high cost, high energy consumption, and strict requirements on quality deviation; flocculation and precipitation The method, hydrophobic adsorption method, and alkaline flocculation and air flotation method all need to add chemical reagents, which not only increases the input, but also affects the recycling of the culture medium.

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  • Method of extracting carotenoid and edible glycerol from Dunaliella sallina

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Embodiment 1

[0022] Under the following main nutrient concentrations, the concentration of sodium bicarbonate is 10mmol / L, the concentration of sodium nitrate is 1mmol / L, the concentration of sodium dihydrogen phosphate is 0.2mmol / L, and the concentration of sodium chloride is 1.5mol / L, culture for 7 days , the biomass is 1 million / ml, the carotenoid content reaches 10 g / m3, and the practical glycerin content reaches 20 g / m3;

[0023] Hollow fiber membrane modules are used to concentrate the above algae liquid. The hollow fiber membrane interception rate is 30KDa. The process adopts cross-flow circulation mode. The circulation pump is a peristaltic pump. The operating pressure is 0.1 bar, the flow rate of algae liquid on the membrane surface is 10m / s, and the membrane flux is 30L. / m 2 h, backwash at 5-hour intervals to maintain the flux, use pH 4.0 acid washing to remove inorganic particles attached to the membrane surface, and sodium hypochlorite to clean the protein attached to the memb...

Embodiment 2

[0026] Under the following main nutrient concentrations, the concentration of sodium bicarbonate is 15mmol / L, the concentration of sodium nitrate is 2mmol / L, the concentration of sodium dihydrogen phosphate is 0.4mmol / L, and the concentration of sodium chloride is 1.5mol / L. Culture for 10 days , with a biomass of 1.5 million / ml, of which the content of carotenoids reaches 20 g / m3, and the content of practical glycerin reaches 30 g / m3;

[0027] The hollow fiber membrane module is used to concentrate the above algae liquid. The hollow fiber membrane interception rate is 5KDa. The process adopts the cross-flow circulation mode. 10L / m 2 h, 1-hour interval backwashing to maintain flux, 1-day intervals with pH 4.0 acid washing to remove inorganic particles attached to the surface of the membrane, sodium hypochlorite to clean the protein attached to the membrane surface, and the concentration factor is 30 times. Use a centrifuge to further concentrate the algae liquid, remove the su...

Embodiment 3

[0030] Under the following main nutrient salt concentrations, the concentration of sodium bicarbonate is 12mmol / L, the concentration of sodium nitrate is 1.4mmol / L, the concentration of sodium dihydrogen phosphate is 0.25mmol / L, and the concentration of sodium chloride is 1.5mol / L. day, the biomass is 1.2 million / ml, the carotenoid content reaches 15 g / m3, and the practical glycerin content reaches 23 g / m3;

[0031] Hollow fiber membrane modules are used to concentrate the above algae liquid, the hollow fiber membrane interception rate is 15KDa, the process adopts cross-flow circulation mode, the circulation pump is a peristaltic pump, the operating pressure is 0.2bar, the flow rate of the algae liquid on the membrane surface is 10m / s, and the membrane flux is 14L / m 2h, backwash at intervals of 2 hours to maintain the flux, use pH 4.0 acid washing to remove inorganic particles attached to the surface of the membrane, and protein attached to the surface of the membrane with so...

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Abstract

A method for extracting carotinoid and edible glycerin from Dunaliella sallina is provided, which comprises the following procedures: optimized nurture of Dunaliella sallina, concentrate the sallina liquid with hollow fiber film method, centrifugal separation, dehydration and drying, extraction of carbon dioxide with super-critical method. The invention adds no chemical reagent in the extraction process, thus ensuring environment-friendly performance, low loss rate of Dunaliella sallina in the extraction process, high recovery rate of carotinoid and edible glycerin, simple process, low cost and suitable for large scale production.

Description

technical field [0001] The invention relates to a method for extracting carotenoids and edible glycerin from Dunaliella salina, in particular to a method for extracting carotenoids and edible glycerin from Dunaliella salina, and belongs to the technical field of biological material preparation. Background technique [0002] Dunaliella Salina is a halophilic planktonic single-celled algae with bare protoplasts. Under the conditions of high salinity, strong light and nitrogen deficiency, the cells can accumulate a large amount of carotene, accounting for up to 14% of the dry weight of the algae. , is the main source of natural carotene. Among them, β-carotene is not only the precursor of vitamin A and the natural pigment of food processing, but also has a strong ability to resist oxidation and eliminate oxygen free radicals, and has anti-cancer and anti-cancer effects. It has been widely used in food, hygiene and the field of medicine. At present, the annual demand for β-car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24A23L1/28A23L1/337A23D9/02A23L33/10
CPCY02P20/54Y02P20/582
Inventor 陈镇杨明德党杰吴玉龙胡湖生刘吉
Owner TSINGHUA UNIV
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