ELISA kit for detecting porcine toxigenic pasteurella multocida toxin antibody and application

A Pasteurella multocida technology, which is applied in the field of ELISA kits for detecting swine toxin-producing Pasteurella multocida toxin antibodies, can solve the problem of not finding antibodies for detecting swine toxin-producing Pasteurella multocida toxins. ELISA kits, biological materials that cannot obtain core reagents, etc., achieve the effects of low production cost, high biological safety, and wide application prospects
CN101012446AInactive Publication Date: 2007-08-08武汉科前动物生物制品有限贵任公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
武汉科前动物生物制品有限贵任公司
Publication Date
2007-08-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an ELISA agent box and usage in the progressive atrophic rhinitis (PAR) as well as rPMT-C recombinant coliform BL21 / pET28b-C2115 (CCTCC NO: M207012) on the C' end of toxigenic pasteurellotic toxin toxA gene and expressing and purifying method of rPMT-C protein, wherein the agent box comprises the following parts: rPMT-C protein on the C' end of toxin toxA gene as enzyme target board, negative, positive standard serum, rabbit anti-swine lgG enzyme target diprevent, sample dilute, substrate display A liquid, substrate display B liquid, stopping liquid and washing liquid.
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Description

technical field

[0001] The invention relates to the technical field of animal bacteriology and animal infectious disease detection. Specifically, the present invention relates to an ELISA kit and application for detecting antibodies to the toxin-producing Pasteurella multocida toxin in pigs. Background technique

[0002] Toxigenic Pasteurella multocida (T. + Pm) is the main pathogen causing progressive atrophic rhinitis (PAR) in pigs. The bacterium secretes and produces a skin necrosis toxin (Pasteurella multocida toxin, PMT) of about 146kD, encoded by the toxA gene, which can directly cause swine rhinitis, nose bridge deformation, turbinate atrophy or even disappearance, systemic metabolic disorders, and production performance decline. Induce infection of other pathogenic microorganisms, even lead to death (Chanter, N et al., Interactions between Bordetella bronchiseptica and toxic Pasteurella multocida in atrophic rhinitis of pigs [J]. Res. Vet. Sci, 1989, 47 (1): 48-53;...

Claims

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