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Membranous tissue engineering rack and its application

A tissue engineering scaffold, membrane-like technology, applied in the field of membrane tissue engineering scaffolds, can solve the problems of fast degradation time, adverse reactions, poor mechanical properties, etc., achieve good mechanical properties, reduce the amount required, and reduce the production of acidic degradation products Effect

Active Publication Date: 2007-11-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, collagen membrane materials (such as BioGide) and hyaluronic acid (such as HYAFF 11) have been used clinically, but their mechanical properties are not good, and the degradation time is too fast; Body adverse reaction

Method used

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  • Membranous tissue engineering rack and its application
  • Membranous tissue engineering rack and its application
  • Membranous tissue engineering rack and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] First prepare a PLLA solution with a low mass concentration (w / v=10%), and the solvent is chloroform / ethanol (v / v=3:1). By electrospinning technology, prepare micron fibers (see Figure 1) (parameters: the range of flow rate=0.5ml / h to 2ml / h, voltage=14KV~18.7KV), and make fiber support with micron fibers. The bone marrow mesenchymal stem was planted on the fibrous scaffold, and the electron microscope observation after 7 days of culture showed that the cells could grow well on the scaffold.

Embodiment 2

[0028] A collagen solution with a concentration of 10 mg / ml was poured onto the fibrous scaffold obtained in Example 1, and frozen at -70° C. for 12 hours. The bilayer material was lyophilized for 24 hours to form a composite material. Finally, vacuum thermal crosslinking is carried out on the composite material: first vacuum pretreatment for 24 hours, during which the temperature is raised by 20°C to 110°C every 6 hours, and then vacuum thermal crosslinking at 110°C for 2 to 4 days. A composite scaffold with good mechanics and sufficient cell attachment surface can be obtained with PLLA as the base membrane and collagen sponge as the guide membrane (see Figures 2-4). (The pore diameter of the obtained PLLA layer is 200-900 nm, and the pore diameter of the collagen layer is 20-500 μm.)

Embodiment 3

[0030] The scaffold obtained in Example 1 was implanted into a white rabbit for repairing tibial defect. After 3 and 6 weeks, it can be found that the tissue grows into the defect site uniformly, forming functional bone tissue.

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Abstract

The present invention provides one kind of membranous tissue engineering rack comprising a microporous layer of fiber material in 20-1000 nm pore size and a macroporous layer of sponge or fiber material in 50-1000 micron pore size and communicated to the microporous layer. The tissue engineering rack of the present invention has the beneficial effects of capacity of blocking fibrocyte invasion with the microporous layer and providing inside cell tissue growth space with the macroporous layer; excellent mechanical performance for minimizing the amount of needed biological polymer material and acid degraded product; and suitability for repairing bone, tendon, cartilage, skin and other tissues.

Description

(1) Technical field [0001] The invention relates to a membrane-like tissue engineering support composed of double-layer biological materials for guided regeneration of human tissue and its application. (2) Background technology [0002] The damage of various tissues of the human body is very common, especially connective tissue damages such as bone, cartilage defect and tendon, ligament are more and more (accounting for 50% of sports injury), and skin damage is countless especially. Statistics show that in 2001, there were 408,000 cases of bone grafting in Europe, while there were 605,000 cases in the United States alone. Currently, the global population over the age of 65 is growing at a rate of 2-3% per year. And due to the improvement of people's material living standards, the change of life style and the improvement of medical level, these have all led to more demands and wider applications of bone transplantation and bone repair surgery. In my country, according to in...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/60A61L27/54A61L31/06
Inventor 欧阳宏伟蔡友治邹晓晖
Owner ZHEJIANG UNIV
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