Method for extracting and purifying dermatan sulfate in angler fish skin

Abstract

It is concerned with extraction and purification of sulfuric acid skin-lotion from waste skins of angler fish production. It is carried out by: extracting with compounded enzyme of pancreatin and papain, adding NaOH into extracting liquid to cut off glycopeptide bond while protecting glycosidic bond with NaPH4, removing protein by savage process, depositing sulfur acid skin lotion in alcohol, dissolving in water and centrifuging dissoluble substances, dialyzing to remove ions and micro-molecular substances, and frozen drying high purity sulfur acid skin lotion with ratio of sulfate radical to carboxylic radical = 1.07. The product is strong antithrombotic, with less side effect of hemorrhage, has disinfecting activity, and can be used in pharmaceutical field.

Description

technical field

[0001] The invention belongs to the technical field of biopharmaceuticals, and relates to the preparation of biomedicine derived from animal bodies, in particular to a method for extracting and purifying dermatan sulfate from anglerfish skin. Background technique

[0002] Dermatan sulfate (DS) is a mucopolysaccharide compound and is the most important glycosaminoglycan in animal skin. Its molecules are mainly composed of double sugar chains composed of N-acetylgalactosamine 4-sulfuric acid and L-iduraldehyde In addition, there are D-glucuronic acid double sugar chains, N-acetylgalactosamine 6-sulfate and D-glucuronic acid double sugar chains. It has strong anticoagulant activity, as well as regulating blood lipids, anti-inflammation, and anti-atherosclerosis. As a drug, it has the characteristics of strong antithrombotic effect and long half-life. Compared with heparin, its outstanding advantage is that it has no bleeding side effects and is well tolerated b...