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Colloidal gold test paper for detecting sulphaquinoxaline drug residue

The technology of sulfaquinoxaline and colloidal gold test paper is applied in the field of immunochemical rapid detection of veterinary drug residues, which can solve the problems of long detection time, high cost, unfavorable promotion and use of basic units, etc., and achieves short detection time, high sensitivity, low price effect

Inactive Publication Date: 2008-03-19
BEIJING WANGER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that the detection time is long and the cost is high, which is not conducive to the promotion and use in grassroots units.
Moreover, both of these methods require professional and technical personnel to operate

Method used

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  • Colloidal gold test paper for detecting sulphaquinoxaline drug residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 SQX detects the preparation of test paper card

[0032] 1. Synthesis and identification of SQX-carrier protein conjugates

[0033] (1) Synthesis of SQX-human serum albumin (HSA) conjugate

[0034] Configure 4mol / L SQX with 0.1mol / L hydrochloric acid solution, add 1% NaNO dropwise 2 (excess), 4°C with constant stirring. NaNO 2 The amount of added starch can be monitored with starch-potassium iodide test paper or by adding 1% starch and 50mol / LKI to white tiles. Free nitrous acid can oxidize iodide to iodine. Iodine then reacts with starch to turn it dark blue. After the solution turned dark blue, the reaction was continued for 15 min. Human serum protein was dissolved with boric acid buffer at pH 8.6 and concentration 2.0 mol / L. Add the diazotized hapten (to prevent local acid excess) while stirring, and adjust the pH to 9.5. The reaction was stirred for 2 h in the refrigerator, and the pH was adjusted to 9.0. Dialyze with PBS for 2 days and store a...

Embodiment 3

[0062] Example 3 Detection of Residues in Samples

[0063] 1. Sample pretreatment

[0064] (1) Animal tissue (chicken, chicken liver, pork, pork liver) samples

[0065] Weigh 2.0±0.05g of the homogenized tissue sample into a centrifuge tube, and tightly cap the bottle. Place the centrifuge tube containing the sample in a slightly boiling water bath for 10 minutes, absorb more than three drops of the boiled solution and pour it into a 1.5ml centrifuge tube. If there is obvious yellow turbidity, please centrifuge, and use the supernatant as the sample solution to be tested.

[0066] (2) Urine sample processing

[0067] Urine sample: Generally, it can be tested directly. When the urine is turbid, filter it with filter paper, and then do spot testing.

[0068] (3) Honey sample processing

[0069] Weigh 2.0±0.05g of honey, put it in a clean container, and add 4ml of borate buffer for dilution.

[0070] 2. Detection with test paper card

[0071] Drop the sample into the hole ...

Embodiment 4

[0077] Example 4 sample detection example

[0078] Take 15 eggs, chicken, and honey samples known to contain SQX concentrations greater than 10ng / g, and 15 negative samples each, and repeat the test twice for each sample, and calculate the negative and positive rates. The results are shown in Table 1, Table 2 and Table 3.

[0079] Table 1 Negative and positive rate detection of egg samples

[0080]

[0081] Table 2 Detection of negative and positive rate of chicken samples

[0082]

[0083] Table 3 Negative and positive rate detection of honey samples

[0084]

[0085] The results show that there are 15 samples of eggs, chicken, and honey, and 15 samples of negative samples. Each sample is tested twice. The false positive rate in eggs is less than 7%. The positive coincidence rate is 100%, and the negative coincidence rate is 93%. %above. The positive and negative coincidence rate of chicken is 100%. The false positive rate in honey is le...

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Abstract

The present invention provides a colloid immunity test paper card for testing medicine sulfaquinoxaline dioxin, and includes a reaction membrane, a sample pad, a combination releasing pad, a drinking pad, and a back pad. The present invention is characterized in that a testing area with a coupling object of the coating sulfaquinoxaline dioxin medicine-carrier albumen and a quality control area ofthe coating goat-anti-mouse lgG are arranged on the reaction membrane. The combination releasing pad coats the sulfaquinoxaline dioxin medicine monoclonal antibodies- colloid metal marker. The present invention also provides a method to use the above medicine sulfaquinoxaline dioxin, and the method includes the following steps: pre-processing the sample, using the test paper card to conduct testing, and analyzing the test result. The test paper card provided by the present invention can be used to test the medicine residue of the sulfaquinoxaline dioxin in the Animal-derived food such as the chicken, the chicken liver, the pork, pig liver, the urine, and the honey. The present invention has simple operation, low cost, high sensitivity, and quick test speed; is applicable for large-scale popularization and mass sample screening; and can be controlled on-spot.

Description

technical field [0001] The invention belongs to the technical field of rapid immunochemical detection of veterinary drug residues, and rapidly detects sulfaquinoxaline drug residues by means of colloidal gold-labeled and color-developed immunochromatographic reactions. Background technique [0002] Sulfaquinoxaline (Sulfaquinoxaline) is a class of chemotherapeutic drugs for the prevention and treatment of bacterial infectious diseases. It has played an important role in the control and treatment of livestock and poultry diseases. However, due to serious side effects, long-term existence in the human body will cause bacteria to develop resistance to sulfaquinoxaline, and has potential carcinogenicity. Therefore, my country, the United States, the European Union, etc. have stipulated that the maximum residue limit (MRLS) of sulfaquinoxaline in food of animal origin is 0.1 mg / kg. [0003] There are currently two commonly used detection methods for SQX residues. One is chroma...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/532C12N5/12
Inventor 沈建忠何方洋万宇平冯才伟赵正苗吴小平冯才茂朱亮
Owner BEIJING WANGER BIOTECH
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