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Preparation method of total glycosides of ranunculus and application thereof

A technology of total glycosides and ranunculus, applied in the field of medicine, to achieve the effect of simple method, high selectivity and strong activity

Inactive Publication Date: 2011-02-16
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the above diseases, although there are many therapeutic drugs, based on the curative effect and adverse reactions, looking for new drugs in this area is still a current and future research hotspot

Method used

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  • Preparation method of total glycosides of ranunculus and application thereof
  • Preparation method of total glycosides of ranunculus and application thereof
  • Preparation method of total glycosides of ranunculus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) 2kg of the whole ranunculus herb was dried and chopped, extracted three times with 14kg of water or 10-95% ethanol or methanol under reflux or percolation, filtered through an 80-mesh sieve, combined the extracts, recovered the solvent under reduced pressure, and concentrated to 1.05kg.

[0036] (2) Add ethyl acetate equal to the volume of the concentrated solution to the concentrated solution of (1) and extract 4 times until the ethyl acetate layer is light green, remove the lower aqueous layer and evaporate the solvent to obtain 200 g of paste;

[0037] (3) 200g paste is adsorbed by D101 macroporous resin, washed with 50% ethanol to 95% methanol, concentrated with 5-10L of alcohol, evaporated to dryness under normal pressure or reduced pressure by conventional methods, to obtain buttercup buttercup The total glycosides product is 100g, and the dry weight of the effective parts of the total ranunculosides in the whole herb of ranunculus is about 5%.

[0038] 2. Com...

Embodiment 2

[0050] Angiotensin II, Sigma, lot number: 125K51011

[0051] DMEM dry powder, Gibco company, batch number: 1324945

[0052] Fetal bovine serum, Hyclone company, batch number: 20060404

[0053] Trypsin, Beijing Dingguo, lot number: DH355-2

[0054] Type II collagenase, Invitrogen, lot number: DH071

[0055] Penicillin, Sigma, lot number: DH231-1

[0056] Streptomycin, Sigma, lot number: DH325

[0057] 5-BrdU, Roche, lot number: 280879

[0058] Sulforhodamine B, Sigma-Aldrich, Lot: 3520-42-1

[0059] Trichloroacetic acid, Genebase, lot number: G2365

[0060] All other reagents were domestic analytically pure

[0061] 2. Instrument

[0062] CO 2 Incubator, Haier Company;

[0063] Microplate reader 850, Biotech laboratories;

[0064] Biological inverted microscope XSP-15C, Shanghai Changfang Optical Instrument Co., Ltd.;

[0065] Low-speed centrifuge LD4-2A, Beijing Medical Centrifuge Factory;

[0066] Vertical pressure steam sterilizer YXQ-LS-30S II, Shanghai Boxun Ind...

Embodiment 3

[0105] 1.1 Instrument

[0106] Biological signal acquisition system (MS4000U), Guangzhou Longfeida Technology Co., Ltd. 10g tension transducer, Beijing Xinhang Xingye Technology and Trade Co., Ltd.

[0107] 1.2 Drugs

[0108] Propranol Hydrochloride Tablets, Shaanxi Yongshou Pharmaceutical Co., Ltd., batch number: 20051028 Isoproterenol Hydrochloride Injection, Shanghai Hefeng Pharmaceutical Co., Ltd., batch number: 5E20004

[0109] 1.3 Animals

[0110] Frogs, about 60-90g, for both ♀♂ and 30 pieces, provided by Guangzhou Suibei Animal Center;

[0111] 2 Experimental methods

[0112] 2.1 Frog heart intubation:

[0113] Refer to the existing experimental method to make an isolated heart specimen of Stuart, then clamp the apex of the heart with a frog heart clip, connect the biological signal acquisition system through the tension transducer, add 1ml of Ren's solution to each cannula, and measure a period of normal heartbeat curves, and perform tests in groups as follows. ...

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Abstract

The invention discloses a ranunculus japonicus total glycoside, which is extracted from ranunculus japonicus whole grass which is used as the raw material, and contains 60%-90% of terpenoid saponin ingredient, and the invention provides a medicament method and the application of the ranunculus japonicus total glycoside, and simultaneously provides the application of the ranunculus japonicus totalglycoside on the aspect of preparing cardiovascular medicine, and applications in preparing medicine which resists myocardium hypertrophy, diastoles blood vessel smooth muscle, inhibits heart action,and eases pain and resists inflammatory. The further research of the ranunculus japonicus total glycoside is to discover novel cardiovascular and a pain-easing antiphlogistic medicine which has high selective performance, strong active performance, small toxic and side effects, which can lay a solid foundation for the extensive application of the ranunculus japonicus total glycoside, and has important potential industrialization developing value and makes significant distribution for human beings in developing novel natural medicine.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for preparing total ranunculoside from the whole ranunculus herb extract and its application in the field of medicine. Background technique [0002] Diseases such as cardiovascular disease and pain inflammation are common clinical diseases. Myocardial hypertrophy is a target organ response to chronic pressure or volume overload, which can lead to decreased myocardial flexibility, cardiac systolic insufficiency, serious cardiovascular events, and increased mortality and disability rates; [0003] Inflammation is a defense response of living tissue with vascular system to injury factors, and its local clinical features are redness, swelling, heat, pain and dysfunction. [0004] Although there are many therapeutic drugs for the above diseases, finding new drugs in this area is still a current and future research hotspot based on the curative effect and adverse reactions....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61P9/00A61P29/00A61K31/704
Inventor 谭毓治周光雄王榕乐
Owner GUANGDONG PHARMA UNIV
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