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Method for separating and purifying erythritol from fermentation liquor

A technology of erythritol and fermented liquid, which is applied in the field of extracting erythritol and using membrane separation technology to purify fermented liquid to separate and purify erythritol, which can solve the problem of low concentration multiple of membrane filtration, low degree of purification of fermented liquid, large Incomplete molecular purification and other problems, to avoid secondary pollution, good separation effect, and reduce membrane fouling load

Inactive Publication Date: 2008-05-21
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned extraction process mainly has the deficiencies in the following aspects: 1. Because the bacterial cell concentration in the fermentation broth is relatively high, directly adopting membrane filtration to separate the bacterial cells will result in low membrane filtration concentration multiples, increased membrane pollution, and shortened membrane life.
In addition, in order to improve the product yield, it is necessary to use a large amount of dialysis water, and the washing water enters the next process to dilute the fermentation liquid, increasing the steam consumption during concentration; 2. Using ion exchange resin to purify the fermentation liquid, resin regeneration needs to consume a lot of acid and alkali, At the same time, the amount of sewage is increased; 3. The ion exchange system does not purify the macromolecules in the fermentation broth thoroughly, and the heat source and other substances that have an impact on food safety are likely to remain in the product; 4. The purification degree of the fermentation broth is low, and the extraction yield is low. The erythritol remains in the mother liquor, causing a waste of resources and increasing the amount of sewage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of erythritol fermentation broth:

[0031] After the slant-preserved strains are activated by the eggplant bottle, they are inoculated in 100L liquid seed culture solution in a conventional amount (glucose 100-200g / L, yeast extract 1-2g / L, potassium dihydrogen phosphate 0.1-0.15g / L, pH natural), cultured at 30°C for 20-24 hours;

[0032] Transfer all the above cultured seed liquids to 2000L seed culture liquid (medium as above), and cultivate in ventilation at 30°C for 16-20 hours;

[0033] Then all the above cultured seed liquids were transferred to 25-30 m 3 In the fermentation medium (glucose 300-350g / L, ammonium citrate 2-5g / L, yeast extract 5-10g / L, potassium dihydrogen phosphate 0.1-0.15g / L, initial pH is natural), at a stirring speed of 120- 150rpm, culture temperature 30-32 ℃, control the dissolved oxygen concentration at about 10% to carry out fermentation, when the glucose in the fermentation liquid is exhausted, the fermentation ends, and the er...

Embodiment 2

[0036] Fermentation broth 25.0m 3 (the content of erythritol is 144.0g / L), and it is centrifuged by a disc-type yeast separator, and then squeezed and washed by a plate frame to obtain a total of 25.5m clear liquid. 3 , the concentration of the supernatant liquid is 140.5g / L, concentrate under reduced pressure at 60°C to 24°Be, cool down and crystallize, the cooling procedure: naturally cool down to crystallization, then cool down by 2-4°C per hour to below 20°C, and centrifuge once to obtain crystallization 1934.7kg (with water), primary crystallization mother liquor 4.45m 3 Discharge, dissolve and decolorize the primary crystallization, separate and desalt, then concentrate and recrystallize to obtain 1236.6kg of recrystallization (water content 3.0%), recrystallization mother liquor 2.94m 3 , the mother liquor returns to the molten crystal for decolorization. The total yield is 52..4%.

Embodiment 3

[0038] Fermentation broth 25.0m 3 (erythritol content 150.0g / L), using 0.2-0.4μm ceramic membrane microfiltration, filtration temperature 70-80℃, operating pressure 0.5-1.0MPa, dialysis water volume 10m 3 , through clear liquid 34.2m 3 , Concentrate under reduced pressure at 60°C to 23.5°Be, crystallize on cooling, cooling procedure: naturally cool down to crystallization, then cool down by 2-4°C per hour to below 20°C, centrifuge to separate 2091.4kg of erythritol crystals, mother liquor Concentrated and crystallized to obtain 821.6kg of erythritol crystals and 3.16m of mother liquor 3 emission. The two crystallizations were combined for crystallization decolorization, separation and desalination, concentration and recrystallization to obtain 1835.2 kg of pure erythritol, with a total yield of 75.8%, and the recrystallization mother liquor was returned to crystallization for decolorization.

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Abstract

The invention discloses a method of separating and purifying erythritol from fermentation liquid, comprising the steps of separating fermentation liquid cell, clarifying and primary purifying the fermentation liquid; decoloring and purifying the fermentation liquid, concentrating and crystallizing the fermentation liquid, crystallizing again for manufacturing the purified product of the erythritol etc. The method of the invention adopts a dish type centrifuge to separate the cell and uses a ceramic film to remove the impurity with quite large molecular weight such as protein etc.; the nano-filtration separation is firstly introduced into the separation and purification of the erythritol, which can dispense with the working procedures of decoloring, ion exchanging etc.; the pollution discharge quantity is reduced greatly, and at the same time the invention ensures the quality and improves the yield of the product. The purity of the erythritol product HPLC obtained by the method of the invention is more thatn 99.5 percent.

Description

technical field [0001] The invention relates to a process for extracting erythritol, in particular to a method for purifying fermented liquid by using membrane separation technology to separate and purify erythritol, which belongs to the field of biochemical industry. Background technique [0002] Erythritol is a tetrahydric alcohol with the chemical name 1,2,3,4-butanetetraol, which occurs naturally in seaweed, mushrooms, melons, grapes and many fermented foods, and has been used as a component of human diet Tens of thousands of years of history. The sweetness of erythritol is 70% of that of sucrose, but the calories are only one-tenth of that of sucrose. Through acute, subacute, chronic toxicity tests and other animal tests and human experiments, it has been confirmed that erythritol is safe and non-toxic, has good food safety, allows a high amount of addition, and is not likely to cause diarrhea or gastrointestinal discomfort. In 1990, Japanese food regulations approved...

Claims

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Application Information

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IPC IPC(8): C07C31/24C07C29/76
Inventor 刘建军赵祥颖田延军张家祥韩延雷刘春生
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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