Method for preparing fluorescent rare earth complex compound silicon nano particles

Abstract

The invention relates to a preparation method of a fluorescent silicon nano-grain of rare-earth complex, belonging to the preparation method of fluorescent silicon nano-grain, in particular to a preparation method which enables the rare-earth complex to circularly link on the surface of the silicon nano-grain to make the fluorescent silicon nano-grain of rare-earth complex. The fluorescent silicon nano-grain of rare-earth complex has the advantages of good control performance, easy achievement, even made grain and strong fluorescence intensity. The preparation method adopts the tactics for multi-layer to modify, namely the fluorescent rare-earth complex is linked on the surface of the silicon nano-grain and circularly grafted by silane, which can make the quantity of the complexes linked on the surface of the silicon nano-grain to maximum and then obtains the fluorescent silicon nano-grain of rare-earth complex with strong fluorescence intensity, good stability and easy marking performance. The fluorescent silicon nano-grain of rare-earth complex adopts the preparation method that the nano-grain is surface functionalized after preparing the carrier of the nano-grain, then the surface radical of the grain is grafted after chemically linking the fluorescent rare-earth complex, finally the fluorescent silicon nano-grain is surface functionalized again after circularly grafting and multi-layer modifying.

Description

technical field [0001] The invention relates to the preparation of fluorescent silicon nanoparticles, in particular to a method for preparing fluorescent rare earth complex silicon nanoparticles by cyclically bonding rare earth complexes on the surface of silicon nanoparticles. Background technique [0002] Commonly used fluorescent markers have a short fluorescence lifetime, which makes it difficult to overcome the interference of background fluorescence and low detection sensitivity. Using rare earth complexes with long-lived fluorescence as markers and time-resolved detection technology can eliminate the interference of background fluorescence and improve sensitivity by several orders of magnitude. This technology called time-resolved fluorescence detection has become the current biomedical Promising ultratrace analytical method in research and clinical biochemical assays. [0003] The existing fluorescent rare earth complex markers, the rare earth ions are mainly Eu 3+...

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Problems solved by technology

Chinese patent CN1298807C discloses a preparation method similar to the above-mentioned patent, but only uses physical doping to dope fluorescent rare earth complexes into silicon nanoparticles

[0013] The preparation methods disclosed in the above patent applications all dope the fluorescent rare earth complexes inside the carrier nanoparticles, and bond the fluorescent rare earth complexes to the surface of the silicon nanoparticle carrier, especially through cyclic grafting. The method of layer modification to bond rare earth complexes to the surface of silicon nanoparticles has not been reported yet.

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