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Baker's yeast and catalytic production method of biodiesel thereof

A biodiesel, baker's yeast technology, applied in the fields of biofuels, fermentation, fungi, etc., can solve the problems of cumbersome immobilization of biological enzymes, non-reusable carriers, and high commodity prices, and achieve good catalytic effect, good elasticity, and mechanical strength. high effect

Inactive Publication Date: 2010-04-14
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1923960 Microbial fermentation oil and its method for preparing biodiesel, using unfixed brewer's yeast, low price, but short service life, easy to inactivate
CN1891786's Chinese tallow tree oil production technology for biodiesel production and CN1730613's biocatalyzed high-acid-value grease for the production of biodiesel by transesterification, the strain used is Candida antarctica, its commodity price is relatively high, and the enzyme activation is cumbersome and volatile live
CN1818026, a method for producing biodiesel by immobilized enzyme method, the immobilization of the biological enzyme is cumbersome, and the carrier cannot be reused
CN1687313 is a process of combining different lipase cells to produce biodiesel. The immobilized cells are Candida antarctic, Thermomyces lanuginosus and Rhizmucor miehei. After immobilization, transesterification is used to prepare biodiesel, but the commodity prices of these immobilized cells are expensive and the enzyme activity is low. unstable, short half-life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Cultivation of baker's yeast strain DX213, the medium is potato medium, the culture time is 1-3 days, the temperature is 25-45°C, centrifuged, the supernatant is taken, and the pH is adjusted to 8 with phosphate buffer to obtain a solution containing baker's yeast , using nano-calcium carbonate as the carrier to adsorb bread yeast to obtain immobilized bread yeast as a catalyst, using rapeseed oil and methanol as raw materials, the oleyl-alcohol molar ratio is 1:6, the catalyst is 1.5% (oil weight), and put into a 250mL three-necked flask , placed in an ultrasonic reactor, the ultrasonic low-frequency radiation power is 200W, the duty cycle is 0.7, the ultrasonic frequency is 30kHz, the reaction temperature is 35°C, the stirring rate is 100rpm, and the reaction is 1.0h. After the reaction is over, the catalyst is separated, the remaining methanol is recovered, and the layers are left to stand for stratification. The upper layer is biodiesel, and the lower layer is glycer...

Embodiment 2

[0018] For the cultivation of baker's yeast strain DX213, the medium is potato medium, the culture time is 1-3 days, the temperature is 25-45°C, centrifuged, the supernatant is taken, and the pH is adjusted to 6.5 with phosphate buffer to obtain the yeast-containing yeast Solution, using hydrotalcite as a carrier to adsorb baker's yeast to obtain immobilized baker's yeast as a catalyst, using soybean oil and methanol as raw materials, the oleyl-alcohol molar ratio is 1:8, the catalyst is 1.2% (oil weight), and put into a 250mL three-necked flask , placed in an ultrasonic reactor, the ultrasonic low-frequency radiation power is 400W, the duty cycle is 0.4, the ultrasonic frequency is 40kHz, the reaction temperature is 30°C, the stirring rate is 100rpm, and the reaction time is 1.2h. After the reaction, the catalyst is separated, the remaining methanol is recovered, and the mixture is poured into a separatory funnel to stand for stratification. The upper layer is biodiesel, and t...

Embodiment 3

[0020] Cultivation of baker's yeast strain DX213, the medium is potato medium, the culture time is 1-3 days, the temperature is 25-45°C, centrifuged, the supernatant is taken, and the pH is adjusted to 7 with phosphate buffer to obtain a solution containing baker's yeast , with γ-Al 2 o 3 The immobilized baker's yeast obtained as a carrier for adsorbing baker's yeast is used as a catalyst, and palm oil and ethanol are used as raw materials. The molar ratio of oleyl to alcohol is 1:12, and the catalyst is 1.2% (oil weight). In the reactor, the ultrasonic low-frequency radiation power is 500W, the duty ratio is 0.7, the ultrasonic frequency is 40kHz, the reaction temperature is 30°C, the stirring rate is 100rpm, and the reaction time is 1.6h. After the reaction is finished, the catalyst is separated, the remaining ethanol is recovered, and the layers are left to stand for stratification. The upper layer is biodiesel, and the lower layer is glycerin. The glycerin is separated to...

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Abstract

The invention discloses a microbial strain which can produce biologic diesel oil by catalyzing vegetable oil, which is bread yeast (Saccharomyces cerevisiae)DX213 CCTCC NO: M 207082, and produce biologic diesel oil by making the microbe into fixed bread yeast as the catalyst. The invention has the following advantages that 1,the price of the lipase cell is low, the fixing process is simple, the fixed yeast cell has certain toxin immunity in the system of exchanging methanol with eater, the catalysis effect is excellent, and the microbial strain is reusable; 2, the fixed lipase cell, which is prepared by using hydrotalcite, quasi hydrotalcite, nano calcium carbonate, gamma-Al2O3, and other loaders as carriers, has excellent flexibility; has strong resistance on temperature and pH value; hashigh mechanical strength, stable enzymatic activity and long half-life; 3 the carrier of the hydrotalcite, quasi hydrotalcite, nano calcium carbonate, gamma-Al2O3 of the fixed lipase cell can be reused after being calcined; 4 the fixed lipase cell cooperates with ultrasonic catalysis to be prepared biologic diesel oil, the process is simple, the condition is moderate, the reaction time is short,the circumstance is friendly, three wastes cannot be produced, and glycerin can be separated easily.

Description

technical field [0001] The invention relates to a baker's yeast (Saccharomyces cerevisiae) and a method for producing biodiesel by using the microorganism as a catalyst. Background technique [0002] With the rapid development of the world economy, the problems of energy and environmental pollution have become increasingly prominent, and human beings are facing enormous pressure on energy and the environment. Today's energy sources are dominated by fossil fuels, including coal, oil and natural gas. The application of fossil energy promotes the development of society, but its resources are increasingly depleted, and at the same time cause serious environmental problems, such as global warming, damage to the ozone layer, destruction of carbon balance in the ecosystem, release of harmful substances, and acid rain. Finding alternative energy sources is a necessary way to solve existing problems. At present, alternative energy sources mainly include nuclear energy, combustible ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P7/64
CPCY02E50/13Y02E50/10
Inventor 曾虹燕邓欣谢凤夏葵廖凯波
Owner XIANGTAN UNIV
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