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Method for preparing biosensor by arraying nanometer particles in external field evoked electrode

A biosensor and nanoparticle technology, which is applied in the field of nanodevices and biosensors, can solve problems such as complex processes and sensor detection limits to be improved, and achieve the effects of high-sensitivity electrical response, simple and easy-to-control devices, and improved sensitivity

Inactive Publication Date: 2008-07-30
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the results obtained using the above biosensors are encouraging, the complex process still needs to be improved and the detection limit of the sensor needs to be improved

Method used

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  • Method for preparing biosensor by arraying nanometer particles in external field evoked electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Metal (gold, silver, platinum, copper, etc.) electrode pairs with nano-gap are processed on silicon-based materials by electron beam lithography or prior art (CN 1560904A). After degreasing the surface of the electrode with an organic solvent (acetone or ethanol), put it into a freshly configured piranha solution (sulfuric acid: hydrogen peroxide = 3: 1, volume ratio) for 15 minutes, take it out, rinse it with water, and use nitrogen flow Blow dry; then put the above electrode into the ethanol solution of the bifunctional molecular mercapto compound X-R-Y (such as mercaptoethylamine or 1,6-hexanedithiol, etc.) for molecular assembly; put the metal electrode assembled with the bifunctional molecule into In the solution of metal nanoparticles (gold, silver, copper, etc.) or II-VI semiconductor nanoparticles, an alternating electric field is applied along the direction of the electrode pair (the peak voltage of the alternating electric field is 10-2000V / cm, an...

Embodiment 2

[0035] Embodiment 2: Electron beam lithography or prior art (CN 1560904A) is used to process magnetic (nickel, cobalt, palladium) electrode pairs with nano-gap on silicon-based materials. After cleaning the surface of the electrode with an organic solvent (acetone or ethanol), put it into an ethanol solution of a bifunctional molecular mercapto compound X-R-Y (such as mercaptoethylamine or 1,6-hexanedithiol, etc.) for molecular assembly; The metal electrodes of functional molecules are placed in the solution of magnetic nanoparticles (nickel, cobalt, palladium, iron and their oxides and derivatives, etc.) with conductor or semiconductor properties, and a static magnetic field (magnetic field strength 1×10 -3 ~3T), induce the magnetic nanoparticles to be arranged in a chain structure at the tip of the electrode pair, connect the corresponding electrode pair, and form a circuit conduction in this direction; then put the electrode into a solution containing sensitive molecules fo...

Embodiment 3

[0036] Embodiment 3: Electron beam lithography or existing technology (CN 1560904A) is used to process magnetic (nickel, cobalt, palladium) electrode pairs with nano-gap on silicon-based materials. After cleaning the surface of the electrode with an organic solvent (acetone or ethanol), put it into an ethanol solution of a bifunctional molecular mercapto compound X-R-Y (such as mercaptoethylamine or 1,6-hexanedithiol, etc.) for molecular assembly; The metal electrodes of functional molecules are placed in a solution of magnetic nanoparticles (nickel, cobalt, palladium, iron and their oxides and derivatives, etc.) with conductor or semiconductor properties, and an alternating magnetic field (magnetic field Intensity range is 1×10 -3 ~3T, the frequency range is 10~10 8 Hz), induce the magnetic nanoparticles to be arranged in a chain-like structure at the tip of the electrode pair, and connect the corresponding electrode pair to form a circuit conduction in this direction; then ...

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Abstract

The invention relates to a method for preparing a biosensor by arraying nanometer grains in an outfield induced electrode. The method is realized by that the nanometer grains form an ordered arrangement in an electrode couple through applying an outfield, and sensitive numerators are combined, so as to develop the biosensor which can be produced in mass and have high sensitivity. The method induces the outfield to the biosensor taking the nanometer grains (b) as a primary element, an alternative electric field (E), or a static magnetic field (B) or an alternating magnetic field (B) which is arranged outside and separated is applied along the direction of a electrode couple (a), the nanometer grains (b) are induced to be arranged in sequence along the direction with biggest electric field or magnetic field grad in the electrode couple (a), a corresponding electrode couple (a) is connected to form a circuit communication in the direction, the sensitive numerators (c) adorned on the nanometer grains (b) has a biochemistry or electrochemistry reaction with a measured objective, a biochemical reaction signal is converted into an electrical signal, the analyte to be measured and the thickness thereof are detected through the amplification and the analog-to-digital conversion of the electric signal.

Description

technical field [0001] The invention belongs to the field of nano devices and biosensors, and relates to a biosensor capable of quantifying matrix contained in samples with high sensitivity. Background technique [0002] With the development of life sciences at the molecular level, scientists predict that the 21st century will be the century of life sciences, and the century of laboratory medicine, that is, the diagnosis and treatment of diseases at the molecular level. This puts forward requirements for clinical biochemical testing that are accurate, fast, simple, micro-specimen, standardized, economical and practical. Biosensors span the fields of biology, chemistry, physics, information, etc., and are an advanced detection method and monitoring method that are indispensable for the development of biotechnology, as well as a rapid and micro-analysis method at the molecular level. A biosensor generally consists of two parts: one is a molecular recognition element (sensitiv...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N27/327
CPCG01N27/3278
Inventor 顾宁黄岚
Owner SOUTHEAST UNIV
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