Method for preparing active chitosan oligosaccharide by using ozone to degrade chitosan

A technology of ozone degradation and chitosan, which is applied in the field of preparation of active chitosan oligosaccharides, can solve the problems of long production cycle, limited degradation degree, and decreased amino group content, and achieve the effects of reduced production cost, shortened reaction cycle, and fast degradation speed

Inactive Publication Date: 2008-08-13
GUANGXI UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, although the acid degradation method is simple in operation, the content of monosaccharides and disaccharides in the degradation products is relatively high, and the content of active chitosan oligosaccharides above three sugars is relatively low. There are also disadvantages such as difficult control of degradation conditions and cumbersome post-treatment.
The enzymatic degradation method has mild conditions, and the degradation process and polymerization degr

Method used

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  • Method for preparing active chitosan oligosaccharide by using ozone to degrade chitosan
  • Method for preparing active chitosan oligosaccharide by using ozone to degrade chitosan

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specific Embodiment 1

[0026] Specific embodiment one: present embodiment prepares active chitosan oligosaccharide according to the following steps: a. the concentration that 20g chitosan (Mw=560kDa, DD=78.5%) is dissolved in 480ml is the acetic acid solution of 2% (mass percentage) , adjust the pH to about 3.0 with hydrochloric acid. b. Place the chitosan solution in a 500ml Erlenmeyer flask, add 5mg Ag / Al 2 o 3 , and then placed in a water bath in an ultrasonic oscillator. c. Open the valve of the compressed oxygen cylinder, open the ozone generator, import the ozone into the chitosan solution by the gas distributor at a speed of 65ml / min, and simultaneously run the agitator to stir at a speed of 750r / min. After reacting for 1 hour, the viscosity of the measured solution had dropped to 10% of the initial value, and the ozone generator was turned off to stop the reaction. d. After microfiltration, the degradation liquid is pumped into an ultrafiltration device with a molecular weight cut-off of ...

specific Embodiment 2

[0027] Specific embodiment two: present embodiment prepares active chitosan oligosaccharide according to the following steps: a. 25g chitosan (Mw=250kDa, DD=90%) is dissolved in the concentration of 475ml and is in the hydrochloric acid solution of 2%. b. Place the chitosan solution in a 500ml Erlenmeyer flask, add 5mg Ni / SiO 2 , and then placed in a water bath in an ultrasonic oscillator. c. Open the valve of the compressed oxygen cylinder, open the ozone generator, import the ozone into the chitosan solution by the gas distributor at a speed of 100ml / min, and simultaneously run the stirrer to stir at a speed of 650r / min. After reacting for 1.5 h, the viscosity of the measured solution had dropped to 8% of the initial value, and the ozone generator was turned off to stop the reaction. d. After microfiltration, the degradation liquid is pumped into an ultrafiltration device with a molecular weight cut-off of 1500 Da, and the degradation products greater than 1500 Da are retur...

specific Embodiment 3

[0028] Specific embodiment three: present embodiment prepares active chitosan oligosaccharide according to the following steps: a. 45g chitosan (Mw=250kDa, DD=85%) is dissolved in the concentration of 955ml and is in the hydrochloric acid solution of 2.5%. b. Put chitosan solution in 1500ml ozone degradation tank, add 10mg Ni / Al 2 o 3, react at room temperature 20°C. c. Open the ultrasonic oscillator of the ozone degradation tank, open the compressed oxygen cylinder valve, open the ozone generator, import the ozone into the chitosan solution by the gas distributor at a speed of 150ml / min, and run the stirrer at the same time at 800r / min stirring speed. After reacting for 1.5 h, the viscosity of the measured solution had dropped to 6% of the initial value, and the ozone generator was turned off to stop the reaction. d. After microfiltration, the degradation liquid is pumped into an ultrafiltration device with a molecular weight cut-off of 2000Da, and the degradation products...

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Abstract

A preparation of chitosan oligosaccharide by degradating chitose with ozone, including steps of: adding acid solution of chitose into ozone degradation groove, adding ozone catalyst, insufflating ozone, reacting for 30-80 min at temperature of 10-100 DEG C. under agitation of high speed and irradiation of ultraviolet to get degradation solution, intercepting degradating product of chitose with degree of polymerization between 3 to 10 or 3 to 20 by membrane separation, concentrating and spray drying to obtain active chitosan oligosaccharide. The invention has advantages of simple operation, faster degradation speed, dramaticly reduced reaction cycle, and production cost is obviously reduced. Ozone is degradated while being decolored, so that no browning of chitosan oligosaccharide occurs. The content of active chitosan oligosaccharide (with degree of polymerization between 3 to 10 or 3 to 20) in the invention is not less than 90%.

Description

technical field [0001] The invention is a technology for preparing active chitosan oligosaccharide by degrading chitosan with ozone. Background technique [0002] Chitin is a polymer with a structure of β-1,4 poly-2-acetylamino-D-glucose, and the product after partial deacetylation is called chitosan. One of the few natural alkaline polysaccharides. Its structural formula is [0003] [0004] Chitosan has special physiological activity, is non-toxic, biodegradable, and has good biocompatibility. In recent years, its application in chemical industry, environmental protection, food, medicine, cosmetics, and agriculture has attracted more and more attention. However, the molecular weight of chitosan varies from hundreds of thousands to millions. And there are a lot of hydrogen bonds between molecules and inside molecules. Therefore, it is insoluble in water and some common solvents, which greatly limits the application of chitosan. [0005] After chitosan is degraded, a...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 魏远安岳武姚评佳何日安
Owner GUANGXI UNIV
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