Method for preparing active chitosan oligosaccharide by using ozone to degrade chitosan

Abstract

A preparation of chitosan oligosaccharide by degradating chitose with ozone, including steps of: adding acid solution of chitose into ozone degradation groove, adding ozone catalyst, insufflating ozone, reacting for 30-80 min at temperature of 10-100 DEG C. under agitation of high speed and irradiation of ultraviolet to get degradation solution, intercepting degradating product of chitose with degree of polymerization between 3 to 10 or 3 to 20 by membrane separation, concentrating and spray drying to obtain active chitosan oligosaccharide. The invention has advantages of simple operation, faster degradation speed, dramaticly reduced reaction cycle, and production cost is obviously reduced. Ozone is degradated while being decolored, so that no browning of chitosan oligosaccharide occurs. The content of active chitosan oligosaccharide (with degree of polymerization between 3 to 10 or 3 to 20) in the invention is not less than 90%.

Description

technical field [0001] The invention is a technology for preparing active chitosan oligosaccharide by degrading chitosan with ozone. Background technique [0002] Chitin is a polymer with a structure of β-1,4 poly-2-acetylamino-D-glucose, and the product after partial deacetylation is called chitosan. One of the few natural alkaline polysaccharides. Its structural formula is [0003] [0004] Chitosan has special physiological activity, is non-toxic, biodegradable, and has good biocompatibility. In recent years, its application in chemical industry, environmental protection, food, medicine, cosmetics, and agriculture has attracted more and more attention. However, the molecular weight of chitosan varies from hundreds of thousands to millions. And there are a lot of hydrogen bonds between molecules and inside molecules. Therefore, it is insoluble in water and some common solvents, which greatly limits the application of chitosan. [0005] After chitosan is degraded, a...

AI Technical Summary

Problems solved by technology

Among them, although the acid degradation method is simple in operation, the content of monosaccharides and disaccharides in the degradation products is relatively high, and the content of active chitosan oligosaccharides above three sugars is relatively low. There are also disadvantages such as difficult control of degradation conditions and cumbersome post-treatment.

The enzymatic degradation method has mild conditions, and the degradation process and polymerization degree are easy to control, but most single hydrolytic enzymes have limited degradation of chitosan, and the hydrolysis time is too long, and the production cycle is too long

h 2 o 2 The speed of oxidative degradation method is relatively fast, but its reaction conditions are difficult to control. It was found that H 2 o 2 Oxidation method will cause the decrease of amino group content, and the late stage of the degradation process is often accompanied by browning reaction

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